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Questions and Answers
DNA strands are composed of
The backbones in DNA run in opposite directions called
DNA is read along the backbone
DNA Complimentary bases held together via
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Each nucleotide in the chain is connected via
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nucleotide linkage yields ____ at physiological pH
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In DNA adenine pairs with
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In DNA cytosine pairs with?
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During storage, DNA wraps around histone proteins — positively charged proteins called?
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This histone-wrapped DNA is stored in a highly condensed structure called
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_________is tightly packed and inactive
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____is unwound and active
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DNA synthesis occurs during the what phase?
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During DNA replication the chromosomes
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DNA serves as the template for its own
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3 steps of DNA Replication
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where does Initiation occur?
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Ori’s rich in what pairs?
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DNA replication initiates at a ____ site prokaryotes
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DNA replication initiates at ____ in eukaryotes
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what are initiation proteins responsible for?
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what are the Initiation Proteins in Prokaryotes
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DnaA Protein
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In DnaA binding causes AT-rich regions to?
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In DnaA melting and strand separation lead to?
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DNA Helicase binds
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what doees DNA Helicase do?
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DNA Helicase requires energy
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DNA Helicase results in?
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Single-stranded Binding (SSB) Proteins bind
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The binding of first SSB protein facilitates the binding of other SSB proteins is called
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In Single-stranded Binding (SSB) Protein strands are kept
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Single-stranded Binding (SSB) Proteins protect Dna from?
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What does Topoisomerase do?
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Topoisomerase I
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Topoisomerase II:
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The site of replication called the
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DNA pol III
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The leading strand synthesized continuously in what direction?
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Lagging strand discontinuously (Okazaki fragments) in the
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DNA Polymerase requires
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Primer
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What does the enzyme primase do ?
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DNA pol removes
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Leading strand grows
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Lagging strand discontinuous growth
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lagging strands is copied in the direction ____ from fork
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Okazaki fragments are joined by ligase
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Lagging strand looped to pass through polymerase active site in the
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DNA polymerase I removes
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DNA ligase joins
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DNA pol III has the capability in what direction
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DNA polymerase "elongates" DNA by
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what are dNTP?
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An incoming nucleoside triphosphate is correctly matched to its complimentary base on the DNA template and is added as the monophosphate to the growing DNA chain which direction will this go?
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If DNA polymerase mispairs a nucletoide with the template it uses what to exonuclease activity to excise the mismatched nucleotide.
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Termination Utilization Substance (Tus) protein binds to
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