Podcast
Questions and Answers
DNA strands are composed of
DNA strands are composed of
The backbones in DNA run in opposite directions called
The backbones in DNA run in opposite directions called
DNA is read along the backbone
DNA is read along the backbone
DNA Complimentary bases held together via
DNA Complimentary bases held together via
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Each nucleotide in the chain is connected via
Each nucleotide in the chain is connected via
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nucleotide linkage yields ____ at physiological pH
nucleotide linkage yields ____ at physiological pH
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In DNA adenine pairs with
In DNA adenine pairs with
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In DNA cytosine pairs with?
In DNA cytosine pairs with?
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During storage, DNA wraps around histone proteins — positively charged proteins called?
During storage, DNA wraps around histone proteins — positively charged proteins called?
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This histone-wrapped DNA is stored in a highly condensed structure called
This histone-wrapped DNA is stored in a highly condensed structure called
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_________is tightly packed and inactive
_________is tightly packed and inactive
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____is unwound and active
____is unwound and active
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DNA synthesis occurs during the what phase?
DNA synthesis occurs during the what phase?
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During DNA replication the chromosomes
During DNA replication the chromosomes
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DNA serves as the template
for its own
DNA serves as the template for its own
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3 steps of DNA Replication
3 steps of DNA Replication
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where does Initiation occur?
where does Initiation occur?
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Ori’s rich in what pairs?
Ori’s rich in what pairs?
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DNA replication initiates at a ____ site prokaryotes
DNA replication initiates at a ____ site prokaryotes
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DNA replication initiates at ____ in eukaryotes
DNA replication initiates at ____ in eukaryotes
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what are initiation proteins responsible for?
what are initiation proteins responsible for?
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what are the Initiation Proteins in Prokaryotes
what are the Initiation Proteins in Prokaryotes
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DnaA Protein
DnaA Protein
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In DnaA binding causes AT-rich regions to?
In DnaA binding causes AT-rich regions to?
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In DnaA melting and strand separation lead to?
In DnaA melting and strand separation lead to?
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DNA Helicase binds
DNA Helicase binds
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what doees DNA Helicase do?
what doees DNA Helicase do?
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DNA Helicase requires energy
DNA Helicase requires energy
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DNA Helicase results in?
DNA Helicase results in?
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Single-stranded Binding (SSB) Proteins bind
Single-stranded Binding (SSB) Proteins bind
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The binding of first SSB protein facilitates the binding of other SSB proteins is called
The binding of first SSB protein facilitates the binding of other SSB proteins is called
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In Single-stranded Binding (SSB) Protein strands are kept
In Single-stranded Binding (SSB) Protein strands are kept
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Single-stranded Binding (SSB) Proteins protect Dna from?
Single-stranded Binding (SSB) Proteins protect Dna from?
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What does Topoisomerase do?
What does Topoisomerase do?
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Topoisomerase I
Topoisomerase I
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Topoisomerase II:
Topoisomerase II:
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The site of replication called the
The site of replication called the
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DNA pol III
DNA pol III
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The leading strand synthesized continuously in what direction?
The leading strand synthesized continuously in what direction?
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Lagging strand discontinuously (Okazaki fragments) in the
Lagging strand discontinuously (Okazaki fragments) in the
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DNA Polymerase requires
DNA Polymerase requires
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Primer
Primer
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What does the enzyme primase do ?
What does the enzyme primase do ?
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DNA pol removes
DNA pol removes
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Leading strand grows
Leading strand grows
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Lagging strand discontinuous growth
Lagging strand discontinuous growth
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lagging strands is copied in the direction ____ from fork
lagging strands is copied in the direction ____ from fork
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Okazaki fragments are joined by ligase
Okazaki fragments are joined by ligase
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Lagging strand looped to pass through polymerase active site in the
Lagging strand looped to pass through polymerase active site in the
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DNA polymerase I removes
DNA polymerase I removes
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DNA ligase joins
DNA ligase joins
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DNA pol III has the capability in what direction
DNA pol III has the capability in what direction
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DNA polymerase "elongates" DNA by
DNA polymerase "elongates" DNA by
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what are dNTP?
what are dNTP?
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An incoming nucleoside triphosphate is correctly matched to its complimentary base on the DNA template and is added as the monophosphate to the growing DNA chain which direction will this go?
An incoming nucleoside triphosphate is correctly matched to its complimentary base on the DNA template and is added as the monophosphate to the growing DNA chain which direction will this go?
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If DNA polymerase mispairs a nucletoide with the template it uses what to exonuclease activity to excise the mismatched nucleotide.
If DNA polymerase mispairs a nucletoide with the template it uses what to exonuclease activity to excise the mismatched nucleotide.
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Termination Utilization Substance (Tus) protein binds to
Termination Utilization Substance (Tus) protein binds to
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