Cytology Lecture Two

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Questions and Answers

Which of the following is NOT a function performed by cells?

  • Photosynthesis (correct)
  • Movement
  • Reproduction
  • Metabolism

Which type of cell is primarily responsible for the synthesis and secretion of mucus?

  • Mucous-gland cells (correct)
  • Pancreatic acinar cells
  • Muscle cells
  • Adrenal cells

What does the cytoplasm of a eukaryotic cell primarily consist of?

  • Nucleus and cell membrane combined
  • Fluid and organelles excluding the nucleus (correct)
  • Cell membrane only
  • Exclusively the nucleus

Which of the following statements is true about muscle cells?

<p>They are specialized for movement. (A)</p> Signup and view all the answers

What aspect differentiates specialized cells from basic cells?

<p>Specialized cells perform unique functions related to specific activities. (C)</p> Signup and view all the answers

What is the primary function of cells in the intestine?

<p>Metabolite absorption (D)</p> Signup and view all the answers

Which of the following cell types is involved in ion transport?

<p>Kidney and salivary gland duct cells (C)</p> Signup and view all the answers

How many named cell types are there in the human body?

<p>Over 250 (D)</p> Signup and view all the answers

What is the primary function of the inner nuclear membrane?

<p>Scaffolding for chromatin (A)</p> Signup and view all the answers

Which pigment is primarily found in melanocytes and keratinocytes?

<p>Melanin (D)</p> Signup and view all the answers

What type of pigment increases with age as residual bodies of lysosomal activities?

<p>Lipofuscin (B)</p> Signup and view all the answers

What is contained within a nucleosome?

<p>146-166 base pairs of DNA (C)</p> Signup and view all the answers

Crystals of Reinke can be found in which type of cells?

<p>Leydig cells (D)</p> Signup and view all the answers

The outer nuclear membrane is connected to which structure?

<p>Endoplasmic Reticulum (D)</p> Signup and view all the answers

Which component is NOT part of the chromatin structure?

<p>Nucleoplasm (C)</p> Signup and view all the answers

What defines the role of lipofuscin in cells?

<p>Residual byproduct of cellular metabolism (C)</p> Signup and view all the answers

What is one of the main functions of the Golgi complex?

<p>Synthesis of polysaccharides (D)</p> Signup and view all the answers

What characteristic distinguishes lysosomes from peroxisomes?

<p>Peroxisomes are larger in diameter than lysosomes. (B)</p> Signup and view all the answers

Which type of biological structure is involved in the packaging of secretory products?

<p>Golgi complex (B)</p> Signup and view all the answers

What pH level is most favorable for the activity of lysosomal enzymes?

<p>Acidic pH (5.0) (C)</p> Signup and view all the answers

What are two key components found within the matrix space of the mitochondria?

<p>Both A and C (D)</p> Signup and view all the answers

What type of vesicle is responsible for the ingested materials within a cell?

<p>Phagosomes (D)</p> Signup and view all the answers

How do peroxisomes replicate?

<p>Budding from precursor vesicles (A)</p> Signup and view all the answers

What is a common characteristic of mitochondria?

<p>Ability to rapidly change shape (C)</p> Signup and view all the answers

What is the primary function of proteasomes?

<p>Degrade proteins tagged with ubiquitin (C)</p> Signup and view all the answers

Which of the following best describes the composition of ribosomes?

<p>Consist of RNA and proteins, with large and small subunits (B)</p> Signup and view all the answers

Polyribosomes or polysomes are significant for which of the following reasons?

<p>They cluster along a single strand of mRNA for efficient protein synthesis. (C)</p> Signup and view all the answers

What role do microtubule-associated proteins (MAPs) play?

<p>Facilitate intracellular transport of vesicles (C)</p> Signup and view all the answers

What structural property differentiates intermediate filaments from microtubules and microfilaments?

<p>They confer increased mechanical stability and tensile strength. (C)</p> Signup and view all the answers

Which type of protein is primarily involved in the elongation of axons?

<p>Dynamin (A)</p> Signup and view all the answers

Which form of polyribosomes synthesizes proteins for cellular use?

<p>Free polyribosomes (C)</p> Signup and view all the answers

What is a common characteristic of microtubules?

<p>They are involved in intracellular compartmentalization. (D)</p> Signup and view all the answers

What is the average thickness of the cell membrane?

<p>7.5 nm (A)</p> Signup and view all the answers

Which type of protein is known to be loosely associated with the inner membrane surface?

<p>Peripheral membrane proteins (D)</p> Signup and view all the answers

Which component comprises the majority of the cell membrane by volume?

<p>Lipids (B)</p> Signup and view all the answers

What is the function of cholesterol in the cell membrane?

<p>Modulates fluidity and structural integrity (D)</p> Signup and view all the answers

Which type of carbohydrate is typically attached to proteins in the plasma membrane?

<p>Oligosaccharides (A)</p> Signup and view all the answers

What characterizes the amphipathic nature of phospholipids?

<p>They contain both hydrophilic heads and hydrophobic tails. (D)</p> Signup and view all the answers

Which of the following is NOT a category of membrane proteins?

<p>Transporters (D)</p> Signup and view all the answers

What is the primary function of carbohydrates on the plasma membrane?

<p>To create a cell coat involved in adhesion (A)</p> Signup and view all the answers

What type of cells contain vimentin?

<p>Mesenchymal-derived cells (B)</p> Signup and view all the answers

Which structure is primarily involved in the control of microtubule polymerization?

<p>Microtubule-organizing centers (MTOCs) (C)</p> Signup and view all the answers

What is the main function of microfilaments in cells?

<p>Transporting organelles and vesicles (B)</p> Signup and view all the answers

Which of the following best describes glial fibrillary acidic protein?

<p>Found in glial cells (C)</p> Signup and view all the answers

Which of the following statements about centrioles is incorrect?

<p>They consist of single microtubules. (A)</p> Signup and view all the answers

Which inclusion is primarily responsible for storing triglycerides and cholesterol in adipose cells?

<p>Lipid droplets (B)</p> Signup and view all the answers

What is the primary role of neurofilaments in the cells?

<p>Forming the cytoskeleton of neurons (D)</p> Signup and view all the answers

Which type of pigment is hemosiderin, and where is it typically found?

<p>A brown pigment in macrophages (C)</p> Signup and view all the answers

Flashcards

Golgi Complex Function

Processes, packages, and transports proteins and lipids.

Lysosome Function

Digests cellular waste and foreign matter.

Peroxisome Function

Detoxifies harmful substances and breaks down lipids; Produce hydrogen peroxide.

Mitochondria Structure

Double-membraned organelles with cristae; contain own DNA.

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Mitochondrial Matrix

Inner space of mitochondria, contains enzymes for energy production.

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Endosome

Membrane-bound vesicle involved in endocytosis and intracellular transport.

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Phagosome Types

Vesicles containing material for digestion; categorized by ingested material.

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Mitochondria DNA

Circular DNA found in the matrix, inherited maternally.

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Cell Membrane Structure

A thin, flexible barrier that surrounds a cell. It's composed of a phospholipid bilayer with embedded proteins and carbohydrates.

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Phospholipid Bilayer

The main structural component of the cell membrane. Two layers of phospholipids arranged with hydrophilic heads facing outward and hydrophobic tails inward.

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Integral Membrane Proteins

Proteins embedded within the cell membrane, some extending completely across the membrane (transmembrane).

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Peripheral Membrane Proteins

Proteins that are loosely associated with the cell membrane, often on the inner or outer surface.

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Membrane Protein Function (Pumps)

Membrane proteins that actively transport substances across the membrane.

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Membrane Protein Function (Channels)

Membrane proteins that form channels for passive movement of substances across the membrane.

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Glycocalyx

A sticky carbohydrate-rich covering on the outer surface of the cell membrane, mostly on proteins and lipids.

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Cholesterol

A lipid component of the membrane that helps maintain its fluidity and stability.

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Cell's function

Cells carry out essential processes for survival, growth, and reproduction, including metabolism (absorption, synthesis, respiration, excretion), growth, regeneration, irritability, movement, reproduction, aging, and death.

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Cell types

There are over 250 types of cells, each with specialized shapes and sizes; this variety corresponds to their diverse functions.

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Cell size

Cells range from 5µm to 120µm in diameter.

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Eukaryotic Cell Components

A eukaryotic cell is made up of three primary components: the cell membrane, cytoplasm, and karyoplasm (nucleus).

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Specialized Cell Functions

Different cells carry out specific tasks, like muscle cells for movement, pancreatic cells for enzyme production, and sensory cells for detecting stimuli.

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Cell Number

The human body contains approximately 100 trillion cells.

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Cell Shape

Cells come in various shapes—round, oval, columnar, and more—reflecting their specialized functions.

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Cell Membrane

The cell membrane is a crucial component of a cell that separates the inside of the cell from the outside.

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Lipofuscin

A yellowish-brown pigment that accumulates in cells as they age, a remnant of lysosomal activity.

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Melanin

A brownish pigment primarily found in skin, hair, and the eyes, responsible for their color. Produced by melanocytes.

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Carbon Particles

Tiny particles of carbon found in macrophages, usually in the lungs, resulting from inhalation.

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Carotenoid

A yellowish-orange-red pigment obtained from plants, primarily fruits and vegetables.

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Crystals in Cells

Crystalline forms of proteins, found in specific cell types. Examples include Reinke crystals in Leydig cells and Charcot-Böttcher crystals in Sertoli cells.

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Proteasome

A cylindrical structure composed of four stacked rings, each containing seven proteins, including proteases. It degrades denatured or nonfunctional proteins tagged with ubiquitin.

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Nucleus

The control center of the cell, containing genetic material (DNA) and responsible for cell function and reproduction.

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Free Polyribosomes

Clusters of ribosomes located in the cytoplasm. They synthesize structural proteins and enzymes for use within the cell.

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Nuclear Envelope

A double membrane surrounding the nucleus, with a space between the membranes called the perinuclear cisternal space.

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Chromatin

A complex of DNA and proteins (histones and non-histones) found within the nucleus, responsible for carrying genetic information.

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Attached Polyribosomes

Clusters of ribosomes attached to the outer nuclear membrane and the endoplasmic reticulum (ER). They produce proteins to be secreted or used in other organelles.

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Cytoskeleton

A network of protein filaments that provides structure and support to the cell. It includes microtubules, intermediate filaments, and microfilaments.

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Microtubule Structure

Hollow tubes made of tubulin proteins. Each microtubule is composed of 13 protofilaments arranged in a cylindrical shape.

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Microtubule Motor Proteins

Proteins associated with microtubules that help move vesicles and organelles. Examples include kinesin and dynein.

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Intermediate Filaments

Tough, rope-like fibers that provide structural support for the cell. They are more stable than microtubules or microfilaments.

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Vimentin

A type of intermediate filament found in mesenchymal cells, which are derived from mesenchyme, the embryonic tissue that gives rise to connective tissues, muscle, and blood vessels.

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Desmin

A specific intermediate filament found in muscle cells. It strengthens and helps organize the muscle fibers, supporting their contraction and relaxation.

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Neurofilaments

Intermediate filaments found in neurons, the nerve cells. They contribute to the structural integrity of nerve fibers, allowing for efficient signal transmission.

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Glial Fibrillary Acid Protein (GFAP)

A type of intermediate filament found in glial cells, which support and protect neurons in the nervous system.

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Microfilaments (Actin Filaments)

Thin, flexible filaments composed of actin protein monomers, arranged in a double helix. They play a vital role in cell movement, muscle contraction, and cell division.

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Centrioles

Small, cylindrical structures found in pairs, perpendicular to each other, near the nucleus. Made of microtubules, they control microtubule organization, play a role in cell division, and form the base of cilia and flagella.

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Inclusions

Metabolically inactive materials within the cell. Examples include lipid droplets, glycogen granules, pigments, and crystals.

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Study Notes

Cytology Lecture Two

  • Cells are the fundamental structural and functional units of living organisms.
  • They exhibit diverse specializations performing survival, growth, and reproduction activities.
  • Cells are self-replicating, self-regulating, and self-governing biological systems.
  • Cellular functions include metabolism (absorption, synthesis, respiration, excretion), growth, regeneration, irritability (excitability), movement, reproduction, and aging and death.
  • There are around 100 trillion (1012) cells in the human body.
  • More than 250 different cell types exist.
  • Cell shapes vary, including round, oval, columnar, multipolar, polygonal, cylindrical, fusiform, pyramidal, and pyriform.
  • Cell size ranges from 5µm to 120µm in diameter.
  • Cells have diverse functions depending on their type.

Cell Components

  • Cell membrane: A trilaminar layer (2.5 nm diameter) acting as a selective barrier.
  • Composed of lipids (50% by weight), proteins (50% by weight), and carbohydrates.
  • Lipids make up 90% of the membrane's volume.
  • Membrane proteins are categorized into integral, peripheral, pumps, channels, receptors, linkers, and enzymes.
  • Phospholipids: Amphipathic molecules forming a bilayer; hydrophilic heads face water, hydrophobic tails face away.
  • Cholesterol: Modulates membrane fluidity and integrity.
  • Carbohydrates: Attached to proteins (glycoproteins) and lipids (glycolipids), forming the glycocalyx, important for cell-cell recognition and interaction.

Cytoplasm

  • The material outside the nucleus and within the plasma membrane.
  • Composed of cytosol (aqueous solution), organelles (specialized structures), cytoskeleton (protein filaments), and inclusions (non-living substances).
  • Cytosol: Contains water, dissolved proteins, salts, sugars, etc.
  • Organelles perform various cellular functions; examples include ER, ribosomes, mitochondria, Golgi complex, endoplasmic reticulum, lysosomes, peroxisomes, proteasomes.
  • Cytoskeleton: Supports and mediates intracellular movement. Subdivided into microtubules, intermediate filaments, and microfilaments.

Organelles

  • Endoplasmic reticulum (ER): A continuous network involved in protein and lipid synthesis.
  • Rough ER has ribosomes, synthesizes proteins for export.
  • Smooth ER: Lacks ribosomes; synthesizes lipids, carbohydrates, and detoxifies substances.
  • Golgi complex (apparatus): Processes, modifies, and sorts proteins and lipids.
  • Lysosomes: Contain hydrolytic enzymes for degrading waste materials.
  • Peroxisomes contain oxidative enzymes breaking down substances like H2O2.
  • Mitochondria: Sites of cellular respiration. Have their own DNA and ribosomes. Contain cristae creating folds.
  • Endosomes and Phagosomes: Membrane-bound structures involved in endocytosis and intracellular transport; including heterophagosomes (ingested) and autophagosomes (self).
  • Proteasomes: Degrade damaged and misfolded proteins.
  • Ribosomes: Sites of protein synthesis. Composed of rRNA and protein subunits (large and small). Free ribosomes or attached to rough ER.
  • Polyribosomes (polysomes): Involved in protein synthesis.

Cytoskeleton

  • Microtubules: Tubular structures involved in cell shape, intracellular transport, and cell division(e.g., formation of the mitotic spindle). Composed of tubulin heterodimers (alpha and beta tubulin).
  • Intermediate filaments: Provide mechanical stability and strength. Formed from tetramers of rod-like proteins.
  • Microfilaments (actin filaments): Thin, flexible protein filaments important for intracellular transport, cytokinesis (cell division). Composed of G-actin.

Centrioles

  • Located near the nucleus, involved in cell division, and the formation of cilia and flagella.
  • Consist of 9 triplets of micro tubules.

Cell Renewal

  • Different cell types vary in their renewal rates.
    • Renewing cells (e.g., skin cells) divide rapidly.
    • Stable cells (e.g., liver cells) divide under certain conditions.
    • Static cells (e.g., nerve cells) do not generally divide.

Cell Cycle

  • A highly regulated series of events leading to cell growth and division including Interphase (Gap 1, S phase, Gap 2), and Mitosis.
  • G1: Cell growth, RNA and protein synthesis.
  • S phase: DNA replication.
  • G2: Cell growth, preparation for cell division.
  • Mitosis: Nuclear division.

Nucleus

  • Oval, elongated, or flattened in shape and contains the genetic material (DNA).
  • Composed of nuclear envelope (double membrane), chromatin, nucleolus (involved in ribosome synthesis), and nucleoplasm (the content of the nucleus other than the chromatin and nucleolus).

Inclusions

  • Metabolically inactive substances, including lipid droplets, glycogen granules, pigments (e.g., melanin, lipofuscin), and crystals.

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