Cytochemistry: Study of Chemical Elements in Cells

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Questions and Answers

What is the primary function of Sudan Black B in differentiating AML from ALL?

  • Staining glycogen and mucoproteins
  • Hydrolyzing esters
  • Staining lipids such as sterols and phospholipids (correct)
  • Detecting Factor VIII-related antigen

Which of the following is a characteristic of esterase staining in myelocytic cells?

  • Stains glycogen and mucoproteins
  • Is specific to granulocytes and monocytes
  • Forms a brightly colored compound with diazonium salt (correct)
  • Is directly proportional to cell growth and maturation

What is the substrate used in the Periodic Acid Schiff (PAS) stain?

  • Naphthol AS-D chloroacetic esters
  • α-napththyl butyrate
  • Glycogen and mucoproteins
  • Carbohydrates and aldehydes (correct)

Which of the following cells is intensely red-pink with the PAS stain?

<p>Platelets (B)</p> Signup and view all the answers

What is the product of the reaction between esterases and diazonium salt?

<p>Brightly colored compound (A)</p> Signup and view all the answers

What is the specificity of the esterase staining technique?

<p>Staining of specifically myelocytic cells (A)</p> Signup and view all the answers

Which enzyme is found in the primary granules of PMNs, eosinophils, and to a certain extent monocytes?

<p>Myeloperoxidase (A)</p> Signup and view all the answers

What is the preferred fixative for non-enzymatic specimens in cytochemistry?

<p>Alcohol (methanol and ethanol) (A)</p> Signup and view all the answers

What type of cells are negative (-) to weakly positive for Myeloperoxidase?

<p>Monocytes (A)</p> Signup and view all the answers

What is the main principle behind Myeloperoxidase (MPX) staining?

<p>MPX oxidizes the substrate in the presence of hydrogen peroxide (B)</p> Signup and view all the answers

Which type of leukemias are characterized by 85% positivity to Myeloperoxidase?

<p>Acute Myeloid Leukemia (AML) (C)</p> Signup and view all the answers

What staining method remains stable even after a month for non-enzymatic specimens?

<p>Periodic Acid Schiff (PAS) stain (D)</p> Signup and view all the answers

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