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Cytochemistry: Study of Chemical Elements in Cells
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Cytochemistry: Study of Chemical Elements in Cells

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Questions and Answers

What is the primary function of Sudan Black B in differentiating AML from ALL?

  • Staining glycogen and mucoproteins
  • Hydrolyzing esters
  • Staining lipids such as sterols and phospholipids (correct)
  • Detecting Factor VIII-related antigen

    • Which of the following is a characteristic of esterase staining in myelocytic cells?

  • Stains glycogen and mucoproteins
  • Is specific to granulocytes and monocytes
  • Forms a brightly colored compound with diazonium salt (correct)
  • Is directly proportional to cell growth and maturation

    • What is the substrate used in the Periodic Acid Schiff (PAS) stain?

  • Naphthol AS-D chloroacetic esters
  • α-napththyl butyrate
  • Glycogen and mucoproteins
  • Carbohydrates and aldehydes (correct)

    • Which of the following cells is intensely red-pink with the PAS stain?

    <p>Platelets</p> Signup and view all the answers

    What is the product of the reaction between esterases and diazonium salt?

    <p>Brightly colored compound</p> Signup and view all the answers

    What is the specificity of the esterase staining technique?

    <p>Staining of specifically myelocytic cells</p> Signup and view all the answers

    Which enzyme is found in the primary granules of PMNs, eosinophils, and to a certain extent monocytes?

    <p>Myeloperoxidase</p> Signup and view all the answers

    What is the preferred fixative for non-enzymatic specimens in cytochemistry?

    <p>Alcohol (methanol and ethanol)</p> Signup and view all the answers

    What type of cells are negative (-) to weakly positive for Myeloperoxidase?

    <p>Monocytes</p> Signup and view all the answers

    What is the main principle behind Myeloperoxidase (MPX) staining?

    <p>MPX oxidizes the substrate in the presence of hydrogen peroxide</p> Signup and view all the answers

    Which type of leukemias are characterized by 85% positivity to Myeloperoxidase?

    <p>Acute Myeloid Leukemia (AML)</p> Signup and view all the answers

    What staining method remains stable even after a month for non-enzymatic specimens?

    <p>Periodic Acid Schiff (PAS) stain</p> Signup and view all the answers

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