Cyanobacteria and C4 Photosynthesis
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Questions and Answers

What structure in carboxysomes helps resist CO2 efflux?

  • A double helix
  • A porous membrane
  • A lipid bilayer
  • An icosahedral protein shell (correct)
  • Which transporter is present in thylakoids that facilitates the uptake of inorganic carbon?

  • Transporters for glucose
  • Transporters for O2
  • Transporters for HCO3- (correct)
  • Transporters for phosphate
  • What is the role of Rubisco in carboxysomes?

  • To transport CO2
  • To synthesize glucose
  • To release oxygen
  • To catalyze the fixation of carbon dioxide (correct)
  • What type of molecular structure does the carboxysome have?

    <p>Icosahedral</p> Signup and view all the answers

    What primary function do transporters for CO2 serve in cyanobacteria?

    <p>Inorganic carbon uptake</p> Signup and view all the answers

    In the C4 cycle proposed by Hatch and Slack, which compound accumulates first after the incorporation of 14CO2?

    <p>Malate</p> Signup and view all the answers

    What is the main role of the compounds that accumulate during the C4 cycle?

    <p>Facilitating carbon fixation</p> Signup and view all the answers

    Which of the following is directly associated with the Rubisco-catalyzed reaction in the context of the C4 cycle?

    <p>Decarboxylation of C4 acids</p> Signup and view all the answers

    What cellular process is primarily affected by the accumulation of 3-PGA in the outlined cycle?

    <p>Carbohydrate formation</p> Signup and view all the answers

    Which component is essential for the initial carboxylation reaction in the C4 cycle?

    <p>CO2</p> Signup and view all the answers

    What role does carbonic anhydrase (CA) play in the chloroplast?

    <p>It converts HCO3- to CO2.</p> Signup and view all the answers

    What is the first product accumulated during the carboxylation reaction described by Hatch and Slack in 1966?

    <p>Malate</p> Signup and view all the answers

    Why is back-diffusion of CO2 slowed in the pyrenoid?

    <p>The starch layer offers resistance.</p> Signup and view all the answers

    In the study by Engel et al. (2015), what technique was used to reveal the native architecture of the Chlamydomonas chloroplast?

    <p>In situ cryo-electron tomography</p> Signup and view all the answers

    Which statement about HCO3- transport is correct?

    <p>There are specific transporters that facilitate its movement into chloroplasts.</p> Signup and view all the answers

    Which compound is labeled first during a pulse-chase study with 14CO2 according to the findings by Hatch and Slack?

    <p>Malate</p> Signup and view all the answers

    What is the permeability of the CO2 molecule across the membrane?

    <p>It is highly permeable to membranes.</p> Signup and view all the answers

    Which of the following statements is NOT true regarding the carbon fixation machinery as discussed in the referenced studies?

    <p>Aspartate is the only product of the carboxylation reaction.</p> Signup and view all the answers

    What does the starch layer in the pyrenoid do?

    <p>It acts as an impermeable barrier.</p> Signup and view all the answers

    What did the study by Liu (2016) focus on in cyanobacteria?

    <p>Biosynthesis and organization of carbon fixation machinery</p> Signup and view all the answers

    How does CO2 concentration occur in the pyrenoids?

    <p>By utilizing carbonic anhydrase and specific transporters.</p> Signup and view all the answers

    Which of the following acids does the 14CO2 label accumulate in first during Hatch and Slack's carboxylation reaction?

    <p>C4 acids</p> Signup and view all the answers

    What impact does HCO3- have on CO2 availability in chloroplasts?

    <p>It serves as the primary carbon source.</p> Signup and view all the answers

    What type of study did Hatch and Slack conduct to explore the carboxylation reaction?

    <p>Pulse-chase study</p> Signup and view all the answers

    Which of the following was NOT mentioned as a product or intermediate in the carbon fixation studies referenced?

    <p>Glucose</p> Signup and view all the answers

    What is the relationship between Rubisco and pyrenoids?

    <p>Rubisco is involved in CO2 fixation in the pyrenoid.</p> Signup and view all the answers

    What type of membrane is permeable to CO2?

    <p>Membranes of chloroplasts are permeable.</p> Signup and view all the answers

    What is the main function of transporters in the pyrenoid?

    <p>To facilitate the transport of bicarbonate into chloroplasts.</p> Signup and view all the answers

    At what temperatures do C3 plants have an advantage over C4 plants?

    <p>10°C</p> Signup and view all the answers

    What is a significant energy requirement for C4 photosynthesis?

    <p>Carboxylation steps</p> Signup and view all the answers

    What happens to photorespiration as temperature increases?

    <p>Increases with temperature</p> Signup and view all the answers

    Why hasn’t C4 photosynthesis evolved in all environments?

    <p>High energy demands</p> Signup and view all the answers

    Which statement accurately describes the relationship between C4 plants and temperature?

    <p>C4 plants thrive in high-temperature environments.</p> Signup and view all the answers

    Study Notes

    Cyanobacteria Carbon Fixation

    • Cyanobacteria use carboxysomes to concentrate carbon dioxide
    • Carboxysomes are icosahedral protein shells with Rubisco packed inside
    • Carboxysomes resist carbon dioxide efflux
    • Cyanobacteria use bicarbonate transporters to bring inorganic carbon into the cell

    Pyrenoids

    • Pyrenoids are organelles used by cyanobacteria and algae for CO2 fixation
    • Pyrenoids concentrate CO2 via transporters and carbonic anhydrase
    • Pyrenoids are surrounded by a starch layer which is impermeable to CO2 and HCO3-
    • CO2 is permeable across the plasma membrane
    • Pyrenoids have specific transporters to move HCO3- through the chloroplast envelope and thylakoids

    C4 Photosynthesis

    • C4 photosynthesis was discovered by Hatch and Slack in 1966. They used pulse-chase experiments with 14C labeled CO2
    • C4 photosynthesis involves a new carboxylation reaction
    • The first product of C4 photosynthesis to be labeled was malate and other C4 acids, then 3-PGA
    • The core of the C4 cycle was proposed by Hatch and Slack in 1966

    C4 vs. C3 Photosynthesis

    • C3 photosynthesis is favored at cooler temperatures
    • C4 photosynthesis requires more energy
    • C4 photosynthesis is favored at high temperatures
    • The main advantage of C3 photosynthesis is that it is less costly in terms of energy use
    • Photorespiration increases as temperature increases for C3 plants
    • C4 plants are favored at high temperatures due to lower levels of photorespiration

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    Lecture 10 C4 Metabolism PDF

    Description

    Explore the fascinating mechanisms of carbon fixation in cyanobacteria and C4 plants. This quiz covers topics such as carboxysomes, pyrenoids, and the discoveries made by Hatch and Slack in 1966. Test your knowledge on how these organisms concentrate carbon dioxide for photosynthesis.

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