15 Questions
Match the following with their respective culture preservation methods:
Periodic transfer to fresh media = Overlaying cultures with mineral oil Limit the availability of O2 and reduce metabolic rate = Maintaining viability of stock culture for a long time Enables removal of some growth under the oil and inoculation in fresh medium = Culture Preservation Method 2 Considerations: time interval of transfers, proper storage temperature = Culture Preservation Method 1
Match the following with their relevant steps in preparing pure cultures:
Transfer desired colony to a slant or stab = Verify the purity under microscope Restreak on agar medium = Physiological and biochemical tests Isolation techniques = Steps in preparing pure cultures Membrane filter technique = Microorganism isolation
Match the following with their descriptions:
Sterile membrane filter with pore size retaining microorganisms = Membrane Filter Technique Overlaying cultures with mineral oil to limit O2 availability = Advantages of Culture Preservation Method 2 Maintaining purity of stock culture over time = Objective of Culture Preservation Methods Types of nutrients used in culture media = Solidifying agents
Match the following culture preservation method with its temperature:
Freeze-drying (lyophilization) = -20oC Freezing with liquid nitrogen = -196oC Drying = 45oC
Match the following culture preservation method with its advantage:
Freeze-drying (lyophilization) = Long-term survival Freezing with liquid nitrogen = Less opportunity for changes in culture characteristics
Match the following culture preservation method with its consideration:
Freeze-drying (lyophilization) = Rapid drying in frozen state Freezing with liquid nitrogen = Cryoprotective agent (glycerol)
Match the following culture preservation method with its limitation:
Drying = For spore- and cyst-formers
Match the following culture preservation method with its source reference:
Freeze-drying (lyophilization) = https://cleanroomtechnology.com/news/article_page/Preserving_reference_cultures_for_QC_and_compliance/88703 Freezing with liquid nitrogen = https://microbeonline.com/maintenance-and-preservation-of-pure-cultures-of-bacteria/
Match the following culture preservation method with its storage requirement:
Freeze-drying (lyophilization) = Small storage containers Freezing with liquid nitrogen = Liquid-nitrogen freezer
Match the following definitions with the correct term:
A culture which contains a single species of microorganism = Pure Culture (Axenic Culture) Extracellular substances which provide the cell with materials for building protoplasm and for energy generation = Nutrients Any nutrient material for growth and cultivation of microorganisms in the laboratory = Culture Medium According to physical state: Liquid (Broth) – no solidifying agent = Liquid
Match the following types of culture media with their solidifying agent:
Solid – 1.5 – 2.0% solidifying agent = Agar or gelatin Semi-solid – 0.1 - 0.5% solidifying agent = Solidifying agent: agar or gelatin According to chemical composition: Synthetic – all components are chemically defined = Synthetic Complex – not all components are chemically defined = Complex
Match the following statements with the correct type of culture media:
For growth and maintenance of microbial cultures = Solid To favor the production of particular compounds = Liquid To study microbial action on some constituents of the medium = Solid According to physical state: Semi-solid = 0.1 - 0.5% solidifying agent
Match the following terms with their definition:
Cultivation increasing the population of microorganisms by providing their nutritional and physical requirements = Nutrients A population of cells arising from a single cell = Pure Culture (Axenic Culture) Liquid culture medium without a solidifying agent = Liquid A culture which contains a single species of microorganism = Pure Culture (Axenic Culture)
Match the following sources with their description:
https://www.asmscience.org/content/education/imagegallery/image.3069 = Definition of Pure Culture (Axenic Culture) https://www.atcc.org/~/media/PDFs/webinars/Presen tations/2017/Best%20Practices%20Microbiology.ashx = Definition of Cultivation https://microbiologyinfo.com/list-of-culture-media-used-in-microbiology-with-their-uses/ = Types and uses of culture medium in microbiology https://www.labtestsguide.com/culture = Types of culture media based on physical state
Match the following content descriptions with their source:
All components are chemically defined in this type of culture media = Synthetic Liquid culture medium without a solidifying agent is found in this source: = http://www.biomerieux-culturemedia.com/product/36-gardnerella-agar Physical states of culture media are detailed in this source: = https://quizlet.com/340178083/motility-test-flash-cards/ Types of culture media based on chemical composition are discussed in this source: = https://www.researchgate.net/figure/Conformation-ofHelicobacter-pylori-through-urease-test_fig3_318021754
Test your knowledge of culture preservation methods used in microbiology laboratories. Learn about techniques such as freeze-drying (lyophilization) and the disadvantages of overlaying cultures with mineral oil.
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