Culture Preservation Methods Quiz

Periodic transfer to fresh media = Overlaying cultures with mineral oil Limit the availability of O2 and reduce metabolic rate = Maintaining viability of stock culture for a long time Enables removal of some growth under the oil and inoculation in fresh medium = Culture Preservation Method 2 Considerations: time interval of transfers, proper storage temperature = Culture Preservation Method 1

Transfer desired colony to a slant or stab = Verify the purity under microscope Restreak on agar medium = Physiological and biochemical tests Isolation techniques = Steps in preparing pure cultures Membrane filter technique = Microorganism isolation

Sterile membrane filter with pore size retaining microorganisms = Membrane Filter Technique Overlaying cultures with mineral oil to limit O2 availability = Advantages of Culture Preservation Method 2 Maintaining purity of stock culture over time = Objective of Culture Preservation Methods Types of nutrients used in culture media = Solidifying agents

<p>Freeze-drying (lyophilization) = -20oC Freezing with liquid nitrogen = -196oC Drying = 45oC</p> Signup and view all the answers

<p>Freeze-drying (lyophilization) = Long-term survival Freezing with liquid nitrogen = Less opportunity for changes in culture characteristics</p> Signup and view all the answers

<p>Freeze-drying (lyophilization) = Rapid drying in frozen state Freezing with liquid nitrogen = Cryoprotective agent (glycerol)</p> Signup and view all the answers

<p>Drying = For spore- and cyst-formers</p> Signup and view all the answers

<p>Freeze-drying (lyophilization) = <a href="https://cleanroomtechnology.com/news/article_page/Preserving_reference_cultures_for_QC_and_compliance/88703">https://cleanroomtechnology.com/news/article_page/Preserving_reference_cultures_for_QC_and_compliance/88703</a> Freezing with liquid nitrogen = <a href="https://microbeonline.com/maintenance-and-preservation-of-pure-cultures-of-bacteria/">https://microbeonline.com/maintenance-and-preservation-of-pure-cultures-of-bacteria/</a></p> Signup and view all the answers

<p>Freeze-drying (lyophilization) = Small storage containers Freezing with liquid nitrogen = Liquid-nitrogen freezer</p> Signup and view all the answers

<p>A culture which contains a single species of microorganism = Pure Culture (Axenic Culture) Extracellular substances which provide the cell with materials for building protoplasm and for energy generation = Nutrients Any nutrient material for growth and cultivation of microorganisms in the laboratory = Culture Medium According to physical state: Liquid (Broth) – no solidifying agent = Liquid</p> Signup and view all the answers

<p>Solid – 1.5 – 2.0% solidifying agent = Agar or gelatin Semi-solid – 0.1 - 0.5% solidifying agent = Solidifying agent: agar or gelatin According to chemical composition: Synthetic – all components are chemically defined = Synthetic Complex – not all components are chemically defined = Complex</p> Signup and view all the answers

<p>For growth and maintenance of microbial cultures = Solid To favor the production of particular compounds = Liquid To study microbial action on some constituents of the medium = Solid According to physical state: Semi-solid = 0.1 - 0.5% solidifying agent</p> Signup and view all the answers

<p>Cultivation increasing the population of microorganisms by providing their nutritional and physical requirements = Nutrients A population of cells arising from a single cell = Pure Culture (Axenic Culture) Liquid culture medium without a solidifying agent = Liquid A culture which contains a single species of microorganism = Pure Culture (Axenic Culture)</p> Signup and view all the answers

<p><a href="https://www.asmscience.org/content/education/imagegallery/image.3069">https://www.asmscience.org/content/education/imagegallery/image.3069</a> = Definition of Pure Culture (Axenic Culture) <a href="https://www.atcc.org/~/media/PDFs/webinars/Presen">https://www.atcc.org/~/media/PDFs/webinars/Presen</a> tations/2017/Best%20Practices%20Microbiology.ashx = Definition of Cultivation <a href="https://microbiologyinfo.com/list-of-culture-media-used-in-microbiology-with-their-uses/">https://microbiologyinfo.com/list-of-culture-media-used-in-microbiology-with-their-uses/</a> = Types and uses of culture medium in microbiology <a href="https://www.labtestsguide.com/culture">https://www.labtestsguide.com/culture</a> = Types of culture media based on physical state</p> Signup and view all the answers

<p>All components are chemically defined in this type of culture media = Synthetic Liquid culture medium without a solidifying agent is found in this source: = <a href="http://www.biomerieux-culturemedia.com/product/36-gardnerella-agar">http://www.biomerieux-culturemedia.com/product/36-gardnerella-agar</a> Physical states of culture media are detailed in this source: = <a href="https://quizlet.com/340178083/motility-test-flash-cards/">https://quizlet.com/340178083/motility-test-flash-cards/</a> Types of culture media based on chemical composition are discussed in this source: = <a href="https://www.researchgate.net/figure/Conformation-ofHelicobacter-pylori-through-urease-test_fig3_318021754">https://www.researchgate.net/figure/Conformation-ofHelicobacter-pylori-through-urease-test_fig3_318021754</a></p> Signup and view all the answers