Culture Media and Purified Water in Microbiology
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Questions and Answers

What is the purpose of chromogens and fluorogens in culture media?

  • To reduce oxygen tension
  • To inhibit bacterial growth
  • To absorb toxic waste products
  • To allow organism differentiation and identification (correct)
  • Which type of bacteria require the free admission of oxygen for growth?

  • Anaerobes
  • Facultative anaerobes
  • Microaerophiles
  • Obligate aerobes (correct)
  • How do microorganisms achieve anaerobic conditions for growth in culture media?

  • By exposing the media to light and air
  • By increasing the amount of oxygen
  • By using reducing substances like sodium thioglycollate (correct)
  • By inoculating into the surface layers of solid media
  • Why is 'free' water necessary for microorganisms in culture media?

    <p>To enhance bacterial growth</p> Signup and view all the answers

    What is the role of protective agents like calcium carbonate in culture media?

    <p>To neutralize and absorb toxic metabolites</p> Signup and view all the answers

    What is the purpose of peptones in culture media?

    <p>To serve as a source of nitrogen and vitamins</p> Signup and view all the answers

    Why are carbohydrates used in culture media?

    <p>To act as energy sources</p> Signup and view all the answers

    Which of the following is a role of buffers in culture media?

    <p>Maintain the pH</p> Signup and view all the answers

    What is the function of selective agents like bile salts in culture media?

    <p>To inhibit gram-negative microorganisms</p> Signup and view all the answers

    Why are solidifying agents like agar added to culture media?

    <p>To change the consistency to a solid or semisolid state</p> Signup and view all the answers

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