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Culture Media and Purified Water in Microbiology

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Questions and Answers

What is the purpose of chromogens and fluorogens in culture media?

To reduce oxygen tension
To inhibit bacterial growth
To absorb toxic waste products
To allow organism differentiation and identification (correct)

Which type of bacteria require the free admission of oxygen for growth?

Anaerobes
Facultative anaerobes
Microaerophiles
Obligate aerobes (correct)

How do microorganisms achieve anaerobic conditions for growth in culture media?

By exposing the media to light and air
By increasing the amount of oxygen
By using reducing substances like sodium thioglycollate (correct)
By inoculating into the surface layers of solid media

Why is 'free' water necessary for microorganisms in culture media?

To enhance bacterial growth (D) Signup and view all the answers

What is the role of protective agents like calcium carbonate in culture media?

To neutralize and absorb toxic metabolites (D) Signup and view all the answers

What is the purpose of peptones in culture media?

To serve as a source of nitrogen and vitamins (B) Signup and view all the answers

Why are carbohydrates used in culture media?

To act as energy sources (C) Signup and view all the answers

Which of the following is a role of buffers in culture media?

Maintain the pH (A) Signup and view all the answers

What is the function of selective agents like bile salts in culture media?

To inhibit gram-negative microorganisms (D) Signup and view all the answers

Why are solidifying agents like agar added to culture media?

To change the consistency to a solid or semisolid state (B) Signup and view all the answers

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