Culture Media and Purified Water in Microbiology

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What is the purpose of chromogens and fluorogens in culture media?

To allow organism differentiation and identification

Which type of bacteria require the free admission of oxygen for growth?

Obligate aerobes

How do microorganisms achieve anaerobic conditions for growth in culture media?

By using reducing substances like sodium thioglycollate

Why is 'free' water necessary for microorganisms in culture media?

To enhance bacterial growth

What is the role of protective agents like calcium carbonate in culture media?

To neutralize and absorb toxic metabolites

What is the purpose of peptones in culture media?

To serve as a source of nitrogen and vitamins

Why are carbohydrates used in culture media?

To act as energy sources

Which of the following is a role of buffers in culture media?

Maintain the pH

What is the function of selective agents like bile salts in culture media?

To inhibit gram-negative microorganisms

Why are solidifying agents like agar added to culture media?

To change the consistency to a solid or semisolid state

Explore the concept of culture media and purified water in microbiology according to the United States Pharmacopeia standards. Learn about the sources of nitrogen and vitamins in culture media such as peptone, protein hydrolysates, infusions, and extracts.

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