Cultura de Cellulas Animal

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Questions and Answers

Qual es le scopo principal de subculturar cellulas in un contexto de cultura cellular?

  • Eliminar productos de excrescentia toxic del medio.
  • Mantener le viabilitate cellular e provider supporto nutritional continu. (correct)
  • Inducer differentiation cellular pro experimentos specific.
  • Reduer le rata de crescimento cellular.

Qual es le rationamento detra le uso de cryopreservation con DMSO o glycerol ante subcultura?

  • Pro inducer un rata de division cellular plus rapide post descongelation.
  • Pro augmentar le adhesion cellular al flask de cultura durante le descongelation.
  • Pro proteger le cellulas ab le formation de cristalles de glacie e mantene lor viabilitate durante le congelation. (correct)
  • Pro modificar geneticamente le cellulas pro un crescimento plus robuste.

Que representa transformation cellular post plus de 80 subculturas?

  • Un augmento del dependentia a factores de crescimento externe.
  • Un declino in le potentia de division celular.
  • Un ritorno al characteristicas del cellulas parental original.
  • Le perdita del sensitivitate a stimulos externe e potential alterationes chromosomal. (correct)

Qual es le implication del requisito de un medio optimal pro le cultura de cellulas mammalian?

<p>Substantias nutritive specific e factores physic e chimic definite es essential pro le crescimento e superviventia. (A)</p> Signup and view all the answers

Qual es le differentia inter cellulas dependente de ancorage e cellulas independente de ancorage?

<p>Cellulas dependente de ancorage requirer adhesion a un superficie pro crescimento, dum cellulas independente de ancorage non requirer. (B)</p> Signup and view all the answers

Como le uso de agentes therapeutic potential es evaluate in cultura cellular?

<p>Per exponer cellulas cultivate al agentes e examinar lor effectos. (B)</p> Signup and view all the answers

Qual es le justification detra le disaggregation de cellulas in le establimento de culturas de cellulas primari?

<p>Pro disrupt le relationes inter cellulas e promover isolation cellular. (B)</p> Signup and view all the answers

Que include le factores physicochimic in cultura cellular mammalian?

<p>pH, pression osmotic, e temperatura. (C)</p> Signup and view all the answers

Como lo cellulas cultivate es usate in manufactura basate in cellulas?

<p>Pro producer productos importante tal como anticorpore monoclonal e hormonos. (D)</p> Signup and view all the answers

Pro que es cellulas usate in le production de vaccinos?

<p>Cellulas pote esser cultivate pro producer vaccinos tal como vaccinos contra polio, hepatitis, e varicella. (D)</p> Signup and view all the answers

Flashcards

Cultura de texito

Le methodo de remover cellulas, texito, o organo de un animal e poner los in un ambiente artificial pro superviventia.

Isolation de cellulas

Cellulas isolate ab texito specific.

Disaggregation de cellulas

Separar cellulas pro disrumper lor relationes normal.

Incubation de cellulas

Mantener conditiones optimal pro crescer cellulas isolate.

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Requisitos nutritional

Medios que da supporto e nutrientes essential.

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Requisitos physicochimic

Factores physic e chimic como pH, pression osmotic e temperatura.

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Subcultura

Transfere de cellulas a medios fresc pro mantenir viabilitate.

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Cultura de cellulas primari

Cellulas chirurgicamente excidite de un organo e cultivate.

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Lineas de cellulas establite

Cellulas subcultivate plus que 70 vices.

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Cellulas transformate

Cellulas que ha le capacitate de crescer indefinite.

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Study Notes

  • Le cultura de cellulas animal require un ambiente optimal pro le crescentia.

Conditiones environmental

  • Le conditiones environmental include demandas nutritional.
  • Un medio debe dar supporto e nutrientes essential tales como aminoacidos, carbohydrate, vitaminas, minerales, factores de crescentia, hormones e gases como CO2 e O2.
  • Le conditiones environmental include demandas physicochimic.
  • Factores como pH, pression osmotic e temperatura controla le factores physic e chimic.

Technicas de cultura

  • Le cultura de cellulas dependente de ancorage (adherente o monolayer) require que alcun cellulas de texito necessita attachemento pro crescentia, como le cellulas del renes.
  • Tote le cellulas derivate de texito normal es dependente de ancorage e necessita superficie o supporto pro lor proliferation.
  • In le absentia de un superficie de supporto, le crescentia es arrestate e le cellulas es destruite a causa del induction de morte cellular programmate.
  • Iste cellulas es sensibile al stress de cisalla e require communication inter cellulas e interaction e via de signalisation pro lor superviventia.
  • Non-dependente de ancorage (cultura de suspension) son cellulas que non require attachamento pro le crescentia o non attacha al superficie del vaso de cultura, p.ex., lymphocytos.
  • Cellulas derivate del systema hematopoietic e cellulas transformate como le cellulas cancere (cellulas de myeloma) es cellulas non-dependente de ancorage e non require superficie o supporto pro crescentia.
  • Cellulas tumoral prolifera in forma suspendite libere de cultura, e recipe plus material nutritional pro un melior crescentia.

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