Cultivation and Isolation of Anaerobic Microbes
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Questions and Answers

What is the main function of the Z-ring in bacterial cells?

  • Initiation of DNA replication
  • Assembly of the cell wall
  • Formation of the septum
  • Establishment of the division site (correct)
  • Which protein is primarily responsible for assisting in septation in bacterial cells?

  • FtsZ (correct)
  • Replisome
  • Min system
  • DNA polymerase
  • How does DNA replication proceed in bacterial cells?

  • Centrally towards the terminus
  • Bidirectionally from the origin (correct)
  • In random directions across the chromosome
  • Unidirectionally from the origin
  • What is the significance of the Terminus in bacterial chromosome replication?

    <p>It signals the end of replication</p> Signup and view all the answers

    Which step comes last in the process of septation in bacterial cells?

    <p>Constriction of cell and septum formation</p> Signup and view all the answers

    What role does the Min system play in bacterial cell division?

    <p>Localizes FtsZ monomers to the division site</p> Signup and view all the answers

    Which method involves microbes being filtered and stained for counting?

    <p>Flow cytometry</p> Signup and view all the answers

    What is the purpose of the Pour Plate Sample technique?

    <p>Counting colonies indirectly</p> Signup and view all the answers

    What is the advantage of using Counting Chambers for microbial counting?

    <p>Cost-effectiveness</p> Signup and view all the answers

    Why are plate count results expressed in terms of colony forming units (CFUs)?

    <p>To differentiate between live and dead cells</p> Signup and view all the answers

    Which technique involves passing microbes through a laser beam one cell at a time?

    <p>Electronic counters</p> Signup and view all the answers

    What is the main benefit of using the Coulter counter for microbial counting?

    <p>Rapid analysis speed</p> Signup and view all the answers

    What is the primary method of reproduction for most bacteria and archaea?

    <p>Binary fission</p> Signup and view all the answers

    In the context of bacterial reproduction, what must all cells do prior to division?

    <p>Replicate and segregate the genome</p> Signup and view all the answers

    How is binary fission different from budding as a reproductive strategy?

    <p>Budding results in unequal growth between mother and daughter cells</p> Signup and view all the answers

    What are the three phases in a typical bacterial cell cycle?

    <p>Growth, chromosome replication, cytokinesis</p> Signup and view all the answers

    What is the role of cytoskeletal filaments in bacterial cell cycles?

    <p>Determining cell shape and aiding in cytokinesis</p> Signup and view all the answers

    How does the cell cycle of bacteria progress from the formation of a new cell to the next division?

    <p>Passage through growth, chromosome replication, and cytokinesis phases</p> Signup and view all the answers

    Why must anaerobic microbes be grown without O2?

    <p>They cannot tolerate O2.</p> Signup and view all the answers

    What is the purpose of the isolation of a pure culture in microbiology?

    <p>To study a single type of microorganism in a mixed culture.</p> Signup and view all the answers

    What is the goal of the streak plate technique in microbiology?

    <p>To obtain individual cells that are separated from each other.</p> Signup and view all the answers

    What is the purpose of an enrichment culture in microbiology?

    <p>To encourage growth of microbes with specific characteristics while inhibiting others.</p> Signup and view all the answers

    What is the spread plate technique used for in microbiology?

    <p>To evenly spread a diluted mixture of cells on an agar plate.</p> Signup and view all the answers

    What elements are considered when setting up an enrichment culture?

    <p>A suitable source of microbes, nutrients, and environmental conditions.</p> Signup and view all the answers

    Which technique involves forcing a microbial suspension through a small orifice with a laser light beam?

    <p>Flow Cytometry</p> Signup and view all the answers

    What is the purpose of staining cells with fluorescent dyes in the direct counts on membrane filters method?

    <p>To distinguish living from dead cells</p> Signup and view all the answers

    Which characteristic of cells can also be counted in flow cytometry besides their number?

    <p>Internal complexity</p> Signup and view all the answers

    What is the main role of the Coulter Counter in electronic counting of microbial cells?

    <p>Measuring electrical resistance as cells pass through a hole</p> Signup and view all the answers

    In viable counting using standard plate counts, how are bacterial samples typically applied for counting?

    <p>Cells are spread over a solid agar surface or mixed with agar and poured into a Petri plate</p> Signup and view all the answers

    What advantage does flow cytometry have over other methods mentioned in the text?

    <p>Capable of counting cells with different characteristics besides number</p> Signup and view all the answers

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