30 Questions
What is the main function of the Z-ring in bacterial cells?
Establishment of the division site
Which protein is primarily responsible for assisting in septation in bacterial cells?
FtsZ
How does DNA replication proceed in bacterial cells?
Bidirectionally from the origin
What is the significance of the Terminus in bacterial chromosome replication?
It signals the end of replication
Which step comes last in the process of septation in bacterial cells?
Constriction of cell and septum formation
What role does the Min system play in bacterial cell division?
Localizes FtsZ monomers to the division site
Which method involves microbes being filtered and stained for counting?
Flow cytometry
What is the purpose of the Pour Plate Sample technique?
Counting colonies indirectly
What is the advantage of using Counting Chambers for microbial counting?
Cost-effectiveness
Why are plate count results expressed in terms of colony forming units (CFUs)?
To differentiate between live and dead cells
Which technique involves passing microbes through a laser beam one cell at a time?
Electronic counters
What is the main benefit of using the Coulter counter for microbial counting?
Rapid analysis speed
What is the primary method of reproduction for most bacteria and archaea?
Binary fission
In the context of bacterial reproduction, what must all cells do prior to division?
Replicate and segregate the genome
How is binary fission different from budding as a reproductive strategy?
Budding results in unequal growth between mother and daughter cells
What are the three phases in a typical bacterial cell cycle?
Growth, chromosome replication, cytokinesis
What is the role of cytoskeletal filaments in bacterial cell cycles?
Determining cell shape and aiding in cytokinesis
How does the cell cycle of bacteria progress from the formation of a new cell to the next division?
Passage through growth, chromosome replication, and cytokinesis phases
Why must anaerobic microbes be grown without O2?
They cannot tolerate O2.
What is the purpose of the isolation of a pure culture in microbiology?
To study a single type of microorganism in a mixed culture.
What is the goal of the streak plate technique in microbiology?
To obtain individual cells that are separated from each other.
What is the purpose of an enrichment culture in microbiology?
To encourage growth of microbes with specific characteristics while inhibiting others.
What is the spread plate technique used for in microbiology?
To evenly spread a diluted mixture of cells on an agar plate.
What elements are considered when setting up an enrichment culture?
A suitable source of microbes, nutrients, and environmental conditions.
Which technique involves forcing a microbial suspension through a small orifice with a laser light beam?
Flow Cytometry
What is the purpose of staining cells with fluorescent dyes in the direct counts on membrane filters method?
To distinguish living from dead cells
Which characteristic of cells can also be counted in flow cytometry besides their number?
Internal complexity
What is the main role of the Coulter Counter in electronic counting of microbial cells?
Measuring electrical resistance as cells pass through a hole
In viable counting using standard plate counts, how are bacterial samples typically applied for counting?
Cells are spread over a solid agar surface or mixed with agar and poured into a Petri plate
What advantage does flow cytometry have over other methods mentioned in the text?
Capable of counting cells with different characteristics besides number
Learn about the cultivation of anaerobic microbes that cannot tolerate oxygen, using anaerobic media and chambers, as well as the isolation of pure cultures allowing the study of single microorganism types. Explore methods like the candle jar technique.
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