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CSF Microbiological Analysis

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Questions and Answers

What is the primary reason why the Slide Culture/Microculture Method is not suitable for dimorphic fungi?

The fungal culture is not preserved in its original state
It is too open and the MOT for this is respiratory (correct)
It requires a high level of skill and time
It is not compatible with the U-shaped glass tube

What is the purpose of placing a sterile filter paper in the petri dish?

To prevent contamination of the culture (correct)
To increase the surface area for the U-shaped glass tube
To support the growth of fungi
To facilitate the removal of the fungal culture

What is the primary function of the inoculating loop in the Slide Culture/Microculture Method?

To remove the fungal culture from the SDA plate
To flame the scalpel
To inoculate the four sides of the glass square with mycelial fragments (correct)
To transfer the block of agar to the glass slide

Why is the petri dish incubated at room temperature for 48 hours?

To observe micellar growth and spore protection (C) Signup and view all the answers

What is the purpose of using 95% LPCB in the Slide Culture/Microculture Method?

To stain the fungal culture (A) Signup and view all the answers

What is the advantage of using the Slide Culture/Microculture Method over other methods?

It is more accurate and preserves the fungal culture in its original state (D) Signup and view all the answers

What is the purpose of flaming the scalpel in the Slide Culture/Microculture Method?

To sterilize the scalpel (D) Signup and view all the answers

What is the purpose of using a U-shaped glass tube in the Slide Culture/Microculture Method?

To hold the sterile filter paper in place (D) Signup and view all the answers

How long does the Slide Culture/Microculture Method take to complete?

48 hours (A) Signup and view all the answers

What is the purpose of using a glass slide and slip in the Slide Culture/Microculture Method?

To observe micellar growth and spore protection (B) Signup and view all the answers