CSF Microbiological Analysis
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Questions and Answers

What is the primary reason why the Slide Culture/Microculture Method is not suitable for dimorphic fungi?

  • The fungal culture is not preserved in its original state
  • It is too open and the MOT for this is respiratory (correct)
  • It requires a high level of skill and time
  • It is not compatible with the U-shaped glass tube

What is the purpose of placing a sterile filter paper in the petri dish?

  • To prevent contamination of the culture (correct)
  • To increase the surface area for the U-shaped glass tube
  • To support the growth of fungi
  • To facilitate the removal of the fungal culture

What is the primary function of the inoculating loop in the Slide Culture/Microculture Method?

  • To remove the fungal culture from the SDA plate
  • To flame the scalpel
  • To inoculate the four sides of the glass square with mycelial fragments (correct)
  • To transfer the block of agar to the glass slide

Why is the petri dish incubated at room temperature for 48 hours?

<p>To observe micellar growth and spore protection (C)</p> Signup and view all the answers

What is the purpose of using 95% LPCB in the Slide Culture/Microculture Method?

<p>To stain the fungal culture (A)</p> Signup and view all the answers

What is the advantage of using the Slide Culture/Microculture Method over other methods?

<p>It is more accurate and preserves the fungal culture in its original state (D)</p> Signup and view all the answers

What is the purpose of flaming the scalpel in the Slide Culture/Microculture Method?

<p>To sterilize the scalpel (D)</p> Signup and view all the answers

What is the purpose of using a U-shaped glass tube in the Slide Culture/Microculture Method?

<p>To hold the sterile filter paper in place (D)</p> Signup and view all the answers

How long does the Slide Culture/Microculture Method take to complete?

<p>48 hours (A)</p> Signup and view all the answers

What is the purpose of using a glass slide and slip in the Slide Culture/Microculture Method?

<p>To observe micellar growth and spore protection (B)</p> Signup and view all the answers

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