CSF and Semen Analysis Overview

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Questions and Answers

What should be done if a semen specimen fails to liquefy within 60 minutes?

Ask the patient for recollection following another proper specimen collection method and criteria.

Add enzymes such as Dulbeco's Phosphate-Buffered Saline and Bromelain that can induce liquefaction.

Report the case and it should appear on the patient result. (correct)

Wait for another 30 minutes until specimen liquefies.

What is the expected change in pH value of a semen specimen that fails to liquefy?

pH is neutral.

It will be within the normal range.

pH will increase given that decreased liquefaction is tantamount to decreased prostatic fluid. (correct)

It will become acidic due to increased prostatic fluid concentration.

In the case of Sarah, what is likely to be observed in the vaginal swab specimen under the microscope?

Motile and moving sperm cells indicating recent assault.

Lethargic sperm head suggesting the assault occurred recently.

Immotile sperm cells observed due to the time elapsed since the assault. (correct)

Remnants of the sperm tail given the time elapsed before testing.

Which of the following incorrectly matches with the inability to collect the last portion of a semen specimen?

Coagulation: Not possible Signup and view all the answers

The presence of 2 or more oligoclonal bands in CSF but not in serum indicates the following, except for?

Neoplastic Disorder Signup and view all the answers

What type of sperm movement is considered ideal for successful fertilization?

4.0 a Signup and view all the answers

Which of the following statements about WBC counting in a CSF specimen is true?

Manual counting uses large squares in Neubauer Counting Chamber. Signup and view all the answers

Which of the following is a common characteristic of a semen specimen that has liquefied?

It is thin and watery. Signup and view all the answers

Which factor does not generally affect fertility?

Abstinence Signup and view all the answers

What is the purpose of the forensic medical exam for Sarah?

To gather evidence for potential legal action. Signup and view all the answers

If a patient's semen sample had a higher pH than normal, what could this indicate?

Infection or inflammation in the reproductive tract. Signup and view all the answers

Which must not be observed in the handling of semen specimens?

Semen specimens can be frozen for later testing. Signup and view all the answers

Which test detects anti-sperm antibodies in Immunoglobulins G, M, and A?

Mixed Agglutination Reaction Signup and view all the answers

What should be a primary concern when collecting a vaginal swab following an assault?

To avoid contamination of the collected specimen. Signup and view all the answers

Which statement regarding sperm count and specimen volume is incorrect?

Sperm count will falsely increase with a partial sample. Signup and view all the answers

Which condition does the presence of oligoclonal bands in CSF typically indicate?

Multiple Sclerosis Signup and view all the answers

Which of the following can occur in Traumatic Tap CSF specimens?

Progressive clarity of blood from first to last tube Signup and view all the answers

What role does 30% albumin play in the cytocentrifugation process?

Improves cell yield by reducing distortion Signup and view all the answers

Which of the following is indicative of an acidic pH in semen?

Infection Signup and view all the answers

Which statement regarding sperm structure is incorrect?

Tail measures 45 cm Signup and view all the answers

Which WHO criteria relate to abstinence for fertility testing?

Abstinence should last 2-3 days, not exceeding 7 days Signup and view all the answers

How is the IgG index calculated given the following values: CSF IgG: 50 mg/dL, Serum IgG: 2 g/dL, CSF Albumin: 70 mg/dL, Serum Albumin: 5 mg/dL?

1.8 Signup and view all the answers

What does an elevated IgG index indicate?

Synthesis of IgG in the CNS Signup and view all the answers

What abnormal condition is associated with an increase of normal cells in cerebrospinal fluid?

Pleocytosis Signup and view all the answers

Study Notes

CSF Specimen Analysis

CSF may clot if there is an increased or clear progressive clot from the first to the last collection tube.
D-dimer is positive in a clotted specimen.
The amount of blood in a specimen is negative in a non-clotted specimen.
Hemosiderin is negative in a non-clotted specimen.
Xanthochromia is negative for non-clotted specimens.

Cytocentrifuge

Adding 30% albumin to CSF specimens improves the cytocentrifugation process.
This process often decreases cellular distortion in viewed specimens.

Semen Analysis

Undevloped seminiferous vesicles are an indication of a semen specimen.
Infection and acidity are indicators for semen analysis.

Semen Abstinence

Abstinence of 2-3 days is necessary for fertility testing.
Specimen collection should not be less than 7 days and not more than 7.

Sperm Morphology

Abnormally shaped sperm cells can be a problem in conception.

CSF IgG Index

CSF IgG is 50 mg/dL; Serum IgG: 2 g/dL; CSF Albumin: 70 mg/dL; Serum Albumin: 5 mg/dL.
- The IgG index is 1.8

Abnormal Blood Cell Conditions

Pleocytosis is an abnormal condition associated with normal cells in the cerebrospinal fluid.

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Description

Explore key concepts in CSF and semen analysis, including the significance of clotting in CSF specimens, the impact of albumin on cytocentrifugation, and the importance of sperm morphology. This quiz covers essential information for understanding fertility testing and cerebrospinal fluid analysis.