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Questions and Answers
Which substance is commonly used for cryopreservation due to its chemical properties?
Which substance is commonly used for cryopreservation due to its chemical properties?
- Liquid nitrogen (correct)
- Liquid helium
- Liquid carbon dioxide
- Liquid oxygen
What is the main effect of cryopreservation on cell viability?
What is the main effect of cryopreservation on cell viability?
- Has no effect on cell viability
- Enhances cell viability
- Decreases cell viability (correct)
- Causes cell mutation
Which technique involves the addition of cryoprotectants to cells and tissues?
Which technique involves the addition of cryoprotectants to cells and tissues?
- Slow freezing
- Vitrification (correct)
- Rapid freezing
- Step-wise freezing
What is the main source of cell damage during cryopreservation?
What is the main source of cell damage during cryopreservation?
Which samples are easier to cryopreserve?
Which samples are easier to cryopreserve?
_______ is the non-lethal storage of biological material at ultra-low temperature: (-196 degree) liquid nitrogen.
_______ is the non-lethal storage of biological material at ultra-low temperature: (-196 degree) liquid nitrogen.
All metabolic activities are ceased during _______.
All metabolic activities are ceased during _______.
Liquid nitrogen is chemically ______, relatively low cost, non toxic, non flammable, and readily available.
Liquid nitrogen is chemically ______, relatively low cost, non toxic, non flammable, and readily available.
The process of cryopreservation involves the collection and preservation of ______ & tissues.
The process of cryopreservation involves the collection and preservation of ______ & tissues.
The addition of _______ is a technique used in cryopreservation.
The addition of _______ is a technique used in cryopreservation.
Flashcards
Cryopreservation
Cryopreservation
Non-lethal storage of biological material at ultra-low temperature (-196 degrees Celsius) using liquid nitrogen.
Vitrification
Vitrification
A technique involving the addition of cryoprotectants to cells and tissues to prevent ice crystal formation.
Liquid Nitrogen Properties
Liquid Nitrogen Properties
Liquid nitrogen is chemically inert, relatively low cost, non-toxic, non-flammable, and readily available.
Process of Cryopreservation
Process of Cryopreservation
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Cryoprotectants
Cryoprotectants
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Liquid nitrogen
Liquid nitrogen
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Cell damage during cryopreservation
Cell damage during cryopreservation
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Effect of cryopreservation on cell viability
Effect of cryopreservation on cell viability
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Metabolic activities during cryopreservation
Metabolic activities during cryopreservation
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Embryos
Embryos
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Study Notes
Cryopreservation
- Cryopreservation is the non-lethal storage of biological material at ultra-low temperature using liquid nitrogen (-196 degrees Celsius).
- Dimethyl sulfoxide (DMSO) is commonly used for cryopreservation due to its chemical properties that help protect cells from damage during freezing.
- During cryopreservation, cell viability is reduced due to the formation of ice crystals that can damage cell membranes and organelles.
- Cryoprotectants are added to cells and tissues to prevent damage from ice crystal formation during cryopreservation.
- Smaller cells and tissues are easier to cryopreserve than larger ones.
- Ice crystal formation is the main source of cell damage during cryopreservation.
- All metabolic activities are ceased during cryopreservation.
- Liquid nitrogen is inert, relatively low cost, non toxic, non flammable, and readily available.
- The process of cryopreservation involves the collection and preservation of cells and tissues.
- Cryoprotectant addition is a technique used in cryopreservation to protect cells from freezing damage.
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