Cryopreservation Quiz

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Questions and Answers

Which substance is commonly used for cryopreservation due to its chemical properties?

  • Liquid nitrogen (correct)
  • Liquid helium
  • Liquid carbon dioxide
  • Liquid oxygen

What is the main effect of cryopreservation on cell viability?

  • Has no effect on cell viability
  • Enhances cell viability
  • Decreases cell viability (correct)
  • Causes cell mutation

Which technique involves the addition of cryoprotectants to cells and tissues?

  • Slow freezing
  • Vitrification (correct)
  • Rapid freezing
  • Step-wise freezing

What is the main source of cell damage during cryopreservation?

<p>Formation of large ice crystals inside the cell (C)</p> Signup and view all the answers

Which samples are easier to cryopreserve?

<p>Embryos (C)</p> Signup and view all the answers

_______ is the non-lethal storage of biological material at ultra-low temperature: (-196 degree) liquid nitrogen.

<p>Cryopreservation</p> Signup and view all the answers

All metabolic activities are ceased during _______.

<p>cryopreservation</p> Signup and view all the answers

Liquid nitrogen is chemically ______, relatively low cost, non toxic, non flammable, and readily available.

<p>inert</p> Signup and view all the answers

The process of cryopreservation involves the collection and preservation of ______ & tissues.

<p>cells</p> Signup and view all the answers

The addition of _______ is a technique used in cryopreservation.

<p>cryoprotectants</p> Signup and view all the answers

Flashcards

Cryopreservation

Non-lethal storage of biological material at ultra-low temperature (-196 degrees Celsius) using liquid nitrogen.

Vitrification

A technique involving the addition of cryoprotectants to cells and tissues to prevent ice crystal formation.

Liquid Nitrogen Properties

Liquid nitrogen is chemically inert, relatively low cost, non-toxic, non-flammable, and readily available.

Process of Cryopreservation

Involves the collection and preservation of cells and tissues at ultra-low temperature.

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Cryoprotectants

Substances added to cells to protect from damage during freezing.

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Liquid nitrogen

Because of their chemical properties, it is commonly used for cryopreservation.

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Cell damage during cryopreservation

The main source of cell damage during cryopreservation is caused by the formation of large ice crystals inside the cell.

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Effect of cryopreservation on cell viability

Cryopreservation causes decreased cell viability of the overall sample.

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Metabolic activities during cryopreservation

During this process all metabolic activities are ceased.

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Embryos

Samples that are easier to cryopreserve.

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Study Notes

Cryopreservation

  • Cryopreservation is the non-lethal storage of biological material at ultra-low temperature using liquid nitrogen (-196 degrees Celsius).
  • Dimethyl sulfoxide (DMSO) is commonly used for cryopreservation due to its chemical properties that help protect cells from damage during freezing.
  • During cryopreservation, cell viability is reduced due to the formation of ice crystals that can damage cell membranes and organelles.
  • Cryoprotectants are added to cells and tissues to prevent damage from ice crystal formation during cryopreservation.
  • Smaller cells and tissues are easier to cryopreserve than larger ones.
  • Ice crystal formation is the main source of cell damage during cryopreservation.
  • All metabolic activities are ceased during cryopreservation.
  • Liquid nitrogen is inert, relatively low cost, non toxic, non flammable, and readily available.
  • The process of cryopreservation involves the collection and preservation of cells and tissues.
  • Cryoprotectant addition is a technique used in cryopreservation to protect cells from freezing damage.

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