CRISPR

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Questions and Answers

Which type of genetic elements can be disrupted using CRISPR?

  • Non-coding elements only
  • Protein-coding genes only
  • Neither protein-coding genes nor non-coding elements
  • Both protein-coding genes and non-coding elements (correct)

What type of genetic variations can be created using CRISPR?

  • Single nucleotide polymorphisms (SNPs) only
  • Both single nucleotide polymorphisms (SNPs) and structural variations (correct)
  • Neither single nucleotide polymorphisms (SNPs) nor structural variations
  • Structural variations only

What can CRISPR be used for in the study of gene function?

  • To create gene knock-out or knock-in animals
  • To target and activate/silence specific genes (correct)
  • To generate disease models in animals and human pluripotent stem cells
  • To study the physiological role of genetic variations

Which genetic elements can be disrupted using CRISPR?

<p>Both protein-coding genes and non-coding elements (D)</p> Signup and view all the answers

What types of genetic variations can be created using CRISPR?

<p>Both SNPs and structural variations (A)</p> Signup and view all the answers

What can CRISPR be modified to do in order to study gene function?

<p>Create gene knock-in animals and bypass the use of embryonic stem cells (A)</p> Signup and view all the answers

What is the advantage of injecting CRISPR directly into one-cell stage embryos?

<p>It bypasses the use of embryonic stem cells (A)</p> Signup and view all the answers

What can CRISPR be used for in the study of gene function?

<p>Both drug target validation and preclinical drug tests (A)</p> Signup and view all the answers

What is the purpose of using CRISPR to create gene knock-out or knock-in animals?

<p>To study the physiological role of genetic variations (C)</p> Signup and view all the answers

Flashcards

CRISPR targets what?

Protein-coding and non-coding elements.

CRISPR creates what?

Single nucleotide polymorphisms (SNPs) and structural variations.

CRISPR studies gene function how?

Target and activate or silence specific genes to study their function.

CRISPR creates what kind of animals?

Create gene knock-in animals & bypass embryonic stem cells.

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Why inject CRISPR into embryos?

It bypasses the use of embryonic stem cells, simplifying the process.

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CRISPR helps drug studies how?

Drug target validation and preclinical drug tests.

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Why create knock-out/in animals?

To study the physiological role of genetic variations.

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Gene Editing

Creating precise and directed changes to the genome.

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Non-coding gene

A gene that does not code for a protein.

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