Counting of Red Blood Corpuscles
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Questions and Answers

What is the purpose of the red blood cell counting method mentioned in the text?

  • To count red blood cells in a given sample (correct)
  • To understand the theory of platelet counting
  • To see leukocytes under the microscope
  • To visualize plasma in blood samples

Which piece of equipment is crucial for red blood cell counting?

  • Petri dishes
  • Test tubes
  • Pipettes (correct)
  • Slides

How is the counting chamber in the hemocytometer designed?

  • With two central platforms and parallel supports for cover glass (correct)
  • With three central platforms
  • With one large square and two small squares
  • With a single platform in the center

What are the dimensions of each small square in the 1 mm^2 square on the counting chamber?

<p>$1/25$ mm$^2$ (A)</p> Signup and view all the answers

What is the purpose of Hayem's solution in red blood cell counting?

<p>To dilute blood samples (C)</p> Signup and view all the answers

Why are the four corner squares of the counting chamber used?

<p>To count leukocytes (B)</p> Signup and view all the answers

What is the correct angle at which the tip of the pipette should touch the space between the cover glass and the counting chamber?

<p>45° (B)</p> Signup and view all the answers

Why should bubbles be avoided during the process of diluting the blood for RBC counting?

<p>To ensure accurate cell counting (D)</p> Signup and view all the answers

How many secondary squares in the counting chamber should be counted for RBCs?

<p>5 (B)</p> Signup and view all the answers

Why is it important to allow 2-3 minutes for the cells to settle before examining them under a microscope?

<p>To ensure an even distribution of cells (B)</p> Signup and view all the answers

What volume does each tertiary square represent in the counting chamber?

<p>$1/400 \times 1/10$ (D)</p> Signup and view all the answers

What is the purpose of counting all cells inside a square and those that touch the left-hand line and baseline (L-shape) when analyzing RBCs?

<p>To ensure a comprehensive count (C)</p> Signup and view all the answers

What formula is used to calculate the number of R.B.Cs per ul of undiluted blood?

<p>No.of cells counted X 400 X 10 X 200 80 (A)</p> Signup and view all the answers

In the formula for calculating R.B.Cs per ul, what does '400' represent?

<p>Area of each tertiary square (C)</p> Signup and view all the answers

Which species has the highest normal count of R.B.Cs per ul?

<p>Sheep (A)</p> Signup and view all the answers

What is the clinical term for a decrease in the number of R.B.Cs below normal levels?

<p>Oligocythemia (C)</p> Signup and view all the answers

If the number of R.B.Cs increases above normal levels, what is the condition called?

<p>Polycythemia (A)</p> Signup and view all the answers

What factor needs to be multiplied by the number of cells in 80 tertiary squares to get the cells per ul of diluted blood?

<p>4000 (B)</p> Signup and view all the answers

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