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18 Questions

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Counting of Red Blood Corpuscles

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Questions and Answers

What is the purpose of the red blood cell counting method mentioned in the text?

To count red blood cells in a given sample (correct)

To understand the theory of platelet counting

To see leukocytes under the microscope

To visualize plasma in blood samples

Which piece of equipment is crucial for red blood cell counting?

Petri dishes

Test tubes

Pipettes (correct)

Slides

How is the counting chamber in the hemocytometer designed?

With two central platforms and parallel supports for cover glass (correct)

With three central platforms

With one large square and two small squares

With a single platform in the center

What are the dimensions of each small square in the 1 mm^2 square on the counting chamber?

$1/25$ mm$^2$ Signup and view all the answers

What is the purpose of Hayem's solution in red blood cell counting?

To dilute blood samples Signup and view all the answers

Why are the four corner squares of the counting chamber used?

To count leukocytes Signup and view all the answers

What is the correct angle at which the tip of the pipette should touch the space between the cover glass and the counting chamber?

45° Signup and view all the answers

Why should bubbles be avoided during the process of diluting the blood for RBC counting?

To ensure accurate cell counting Signup and view all the answers

How many secondary squares in the counting chamber should be counted for RBCs?

5 Signup and view all the answers

Why is it important to allow 2-3 minutes for the cells to settle before examining them under a microscope?

To ensure an even distribution of cells Signup and view all the answers

What volume does each tertiary square represent in the counting chamber?

$1/400 \times 1/10$ Signup and view all the answers

What is the purpose of counting all cells inside a square and those that touch the left-hand line and baseline (L-shape) when analyzing RBCs?

To ensure a comprehensive count Signup and view all the answers

What formula is used to calculate the number of R.B.Cs per ul of undiluted blood?

No.of cells counted X 400 X 10 X 200 80 Signup and view all the answers

In the formula for calculating R.B.Cs per ul, what does '400' represent?

Area of each tertiary square Signup and view all the answers

Which species has the highest normal count of R.B.Cs per ul?

Sheep Signup and view all the answers

What is the clinical term for a decrease in the number of R.B.Cs below normal levels?

Oligocythemia Signup and view all the answers

If the number of R.B.Cs increases above normal levels, what is the condition called?

Polycythemia Signup and view all the answers

What factor needs to be multiplied by the number of cells in 80 tertiary squares to get the cells per ul of diluted blood?

4000 Signup and view all the answers