18 Questions
What is the purpose of the red blood cell counting method mentioned in the text?
To count red blood cells in a given sample
Which piece of equipment is crucial for red blood cell counting?
Pipettes
How is the counting chamber in the hemocytometer designed?
With two central platforms and parallel supports for cover glass
What are the dimensions of each small square in the 1 mm^2 square on the counting chamber?
$1/25$ mm$^2$
What is the purpose of Hayem's solution in red blood cell counting?
To dilute blood samples
Why are the four corner squares of the counting chamber used?
To count leukocytes
What is the correct angle at which the tip of the pipette should touch the space between the cover glass and the counting chamber?
45°
Why should bubbles be avoided during the process of diluting the blood for RBC counting?
To ensure accurate cell counting
How many secondary squares in the counting chamber should be counted for RBCs?
5
Why is it important to allow 2-3 minutes for the cells to settle before examining them under a microscope?
To ensure an even distribution of cells
What volume does each tertiary square represent in the counting chamber?
$1/400 \times 1/10$
What is the purpose of counting all cells inside a square and those that touch the left-hand line and baseline (L-shape) when analyzing RBCs?
To ensure a comprehensive count
What formula is used to calculate the number of R.B.Cs per ul of undiluted blood?
No.of cells counted X 400 X 10 X 200 80
In the formula for calculating R.B.Cs per ul, what does '400' represent?
Area of each tertiary square
Which species has the highest normal count of R.B.Cs per ul?
Sheep
What is the clinical term for a decrease in the number of R.B.Cs below normal levels?
Oligocythemia
If the number of R.B.Cs increases above normal levels, what is the condition called?
Polycythemia
What factor needs to be multiplied by the number of cells in 80 tertiary squares to get the cells per ul of diluted blood?
4000
Learn about the theory and method of counting red blood cells using a hemocytometer under a microscope. Practice counting RBCs in a given sample and understand species variations in RBC count. Explore the process of estimating the number of red cells in diluted blood.
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