Complete Blood Count (CBC) Introduction
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Questions and Answers

What is the percentage of the total volume of a blood sample that is made up of the RBC layer?

  • 60%
  • 25%
  • 45% (correct)
  • 75%

What is the purpose of spinning a blood sample in a centrifuge?

  • To separate the layers of the blood sample according to their density (correct)
  • To mix the blood sample thoroughly
  • To increase the density of the plasma
  • To remove the formed elements from the plasma

What is the term for the thin, whitish layer that contains leukocytes and platelets?

  • RBC layer
  • Buffy coat (correct)
  • Packed cell volume
  • Plasma layer

What is the nonliving fluid matrix that suspends the formed elements in blood?

<p>Plasma (C)</p> Signup and view all the answers

What is the term for the living cells suspended in the plasma of blood?

<p>Formed elements (A)</p> Signup and view all the answers

What is the main reason for separating blood into layers through centrifugation?

<p>To examine the different components of blood (C)</p> Signup and view all the answers

What is the primary component of the buffy coat layer?

<p>Leukocytes and platelets (C)</p> Signup and view all the answers

What is the term for the ratio of the volume of erythrocytes to the total volume of blood?

<p>Hematocrit (D)</p> Signup and view all the answers

What is the term for the living cells suspended in the plasma of blood?

<p>Formed elements (B)</p> Signup and view all the answers

What is the characteristic of the blood components that determines their position in a centrifuged sample?

<p>Density (D)</p> Signup and view all the answers

Study Notes

Blood Composition

  • Blood is a specialized type of connective tissue consisting of living cells (formed elements) suspended in a nonliving fluid matrix called plasma.

Blood Layers

  • When a blood sample is spun in a centrifuge, it separates into layers according to density.
  • The layers, from heaviest to lightest, are:
    • Red Blood Cell (RBC) layer, also known as packed cell volume (PCV) or hematocrit, which makes up 45% of the total blood volume.
    • Buffy coat (a thin whitish layer) containing leukocytes and platelets at the RBC-Plasma junction.

Blood Composition

  • Blood is a specialized type of connective tissue consisting of living cells (formed elements) suspended in a nonliving fluid matrix called plasma.

Blood Sample Layers

  • When spun in a centrifuge, a blood sample separates into layers based on density, with the heaviest at the bottom and the least dense at the top.
  • The three layers that form are:
    • Red blood cells (RBCs or erythrocytes) layer, making up 45% of the total blood sample volume (also known as packed cell volume or hematocrit).
    • Buffy coat, a thin, whitish layer at the RBC-plasma junction, containing leukocytes and platelets.

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Description

Learn about blood tests, the composition of blood, and the separation of blood components through centrifugation.

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