Podcast
Questions and Answers
What is the role of the 3'OH group in RNA synthesis by RNA polymerase?
What is the role of the 3'OH group in RNA synthesis by RNA polymerase?
- It acts as a nucleophile attacking the alpha phosphorous of the incoming NTP. (correct)
- It serves as a template for the addition of the next base.
- It interacts with the 5' end of the mRNA strand.
- It coordinates with divalent metals at the active site.
How does the initiation of RNA synthesis by RNA polymerase differ from DNA polymerase?
How does the initiation of RNA synthesis by RNA polymerase differ from DNA polymerase?
- RNA polymerase uses Mg2+ ions in the mechanism, while DNA polymerase uses Zn2+ ions.
- The 5’ to 3’ directionality of RNA synthesis is opposite to that of DNA synthesis.
- RNA polymerase requires a primer to start synthesis, while DNA polymerase does not. (correct)
- DNA polymerase initiates transcription at the +1 site of the DNA, while RNA polymerase does not have a specific initiation site.
What structural feature of RNA polymerase's active site allows for the correct positioning of the 3'OH group during RNA synthesis?
What structural feature of RNA polymerase's active site allows for the correct positioning of the 3'OH group during RNA synthesis?
- Requirement of primase activity
- Presence of a 5' cap structure
- Interaction with divalent metals that coordinate with the phosphates in the NTP (correct)
- Incorporation of a template strand
What is the main difference between DNA and RNA polymerases?
What is the main difference between DNA and RNA polymerases?
What occurs at the active site of RNA polymerase during elongation?
What occurs at the active site of RNA polymerase during elongation?
Which statement describes the action of RNA polymerase during termination?
Which statement describes the action of RNA polymerase during termination?
In terms of fidelity during synthesis, why are DNA polymerases considered more accurate than RNA polymerases?
In terms of fidelity during synthesis, why are DNA polymerases considered more accurate than RNA polymerases?
What is the role of 3'OH in both RNA and DNA polymerases?
What is the role of 3'OH in both RNA and DNA polymerases?
Why does RNA polymerase have no need for a primer during synthesis?
Why does RNA polymerase have no need for a primer during synthesis?
What is the primary reason for DNA polymerase to have a separate site with exonuclease activity?
What is the primary reason for DNA polymerase to have a separate site with exonuclease activity?
Which enzyme requires NTPs such as ATP, GTP, CTP, and UTP for synthesis?
Which enzyme requires NTPs such as ATP, GTP, CTP, and UTP for synthesis?
In what way are DNA polymerase and RNA polymerase structurally different despite their similar active sites?
In what way are DNA polymerase and RNA polymerase structurally different despite their similar active sites?
What evolutionary concept is consistent with the similarities in the active sites of RNA and DNA polymerases?
What evolutionary concept is consistent with the similarities in the active sites of RNA and DNA polymerases?
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