Colorimetric Analysis Quiz

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Questions and Answers

What is the principle behind colorimetric analysis?

Many substances in solution can be colored or produce a colored derivative (correct)

The Beer-Lambert law states that the amount of light absorbed by a colored solution is directly proportional to the concentration of the solution and the length of the light path through it

The Walden Precision Apparatus (WPA) CO700D medical colorimeter has a light source, filters, photosensitive detector system, and cells (cuvettes) to hold solutions

Colored substances absorb light at specific wavelengths in the visible spectrum

What is the Beer-Lambert law?

The amount of light absorbed by a colored solution is directly proportional to the concentration of the solution and the length of the light path through it (correct)

The visible light spectrum ranges from about 400 nm to 700 nm

The Walden Precision Apparatus (WPA) CO700D medical colorimeter has a light source, filters, photosensitive detector system, and cells (cuvettes) to hold solutions

Colored substances absorb light at specific wavelengths in the visible spectrum

What is the difference between colorimeters and spectrophotometers?

Colorimeters and spectrophotometers both use colored filters to cover wavelength ranges

Colorimeters and spectrophotometers are the same thing

Colorimeters use a diffraction grating or prism to disperse white light into a continuous spectrum and spectrophotometers use colored filters to cover wavelength ranges

Colorimeters use colored filters to cover wavelength ranges and spectrophotometers use a diffraction grating or prism to disperse white light into a continuous spectrum (correct)

What is the primary cause of overweight and obesity?

Lack of energy balance Signup and view all the answers

What is the most widely used measurement for abdominal fat distribution?

Waist-hip ratio Signup and view all the answers

What is the recommended duration for completing weight loss through physical activity?

225-300 minutes per week Signup and view all the answers

Which of the following is NOT a health benefit of weight loss?

Increased blood pressure Signup and view all the answers

What is the risk factor for insulin resistance, diabetes mellitus, and cardiovascular disease in terms of waist circumference?

Greater than 102 cm in men and 88 cm in women Signup and view all the answers

What is the primary cause of overweight and obesity?

Lack of energy balance Signup and view all the answers

Which of the following is a risk factor for insulin resistance, diabetes mellitus, and cardiovascular disease?

Waist circumference greater than 102 cm in men and 88 cm in women Signup and view all the answers

What is the recommended duration of physical activity per week for weight loss?

225-300 minutes Signup and view all the answers

Which of the following is NOT a health benefit of weight loss?

Increased blood pressure Signup and view all the answers

What is the recommended treatment for weight loss in patients with BM > 35 Kg/m2 and severe comorbidities?

Bariatric surgery Signup and view all the answers

Study Notes

Principles of Colorimetric Analysis

Colorimetric analysis is based on the principle that many substances in solution can be colored or produce a colored derivative.
The intensity of color in a solution is related to the amount of substance present, with higher concentrations appearing darker in color.
Colored substances absorb light at specific wavelengths in the visible spectrum, with pale colored solutions absorbing only a small amount of light and dark colored solutions absorbing a lot of light.
The visible light spectrum ranges from about 400 nm to 700 nm, with wavelengths shorter than 400 nm being ultraviolet and longer than 700 nm being infrared.
A substance's ability to selectively absorb certain wavelengths of light is determined by its molecular and atomic structures.
The Beer-Lambert law states that the amount of light absorbed by a colored solution is directly proportional to the concentration of the solution and the length of the light path through it.
Colorimetric tests use the Beer-Lambert law to determine the concentration of a substance in an unknown solution by comparing it to a standard.
The amount of light absorbed (optical density or OD) is dependent on the light path distance and wavelength used.
Colorimeters and spectrophotometers are used to measure absorbance, with colorimeters using colored filters to cover wavelength ranges and spectrophotometers using a diffraction grating or prism to disperse white light into a continuous spectrum.
The Walden Precision Apparatus (WPA) CO700D medical colorimeter has a light source, filters, photosensitive detector system, and cells (cuvettes) to hold solutions.
To ensure accurate results, solutions used in colorimetric tests must be homogenous, the substance being measured should not react with the solvent, and a reagent blank must be used to correct for any absorption of light by solvents.
The Beer-Lambert law can be applied to most clinical chemistry quantitative tests, as long as reliable chemicals and good technique are used to prepare solutions.

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Description

Test your knowledge on the principles of colorimetric analysis with this quiz! From the Beer-Lambert law to the use of colorimeters and spectrophotometers, this quiz will cover everything you need to know about the science behind colorimetric tests. Sharpen your understanding of the visible light spectrum, molecular structures, and absorption of light by taking this quiz. Challenge yourself and see if you can ace it!