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Questions and Answers
What is a characteristic feature of bacteriophages compared to plasmids in bacterial cells?
What is a characteristic feature of bacteriophages compared to plasmids in bacterial cells?
- Bacteriophages are unable to link with foreign DNA.
- Bacteriophages can only replicate in the presence of plasmids.
- Bacteriophages typically exhibit a high number of copies per cell. (correct)
- Bacteriophages have a lower copy number compared to plasmids.
Which statement accurately describes the replication of plasmids within bacterial cells?
Which statement accurately describes the replication of plasmids within bacterial cells?
- Some plasmids can replicate with only one or two copies per cell. (correct)
- Plasmids do not replicate independently of chromosomal DNA.
- Plasmids are only capable of replicating in the presence of bacteriophages.
- All plasmids replicate at the same copy number.
How do engineered vectors facilitate the introduction of foreign DNA?
How do engineered vectors facilitate the introduction of foreign DNA?
- By allowing easier linking of foreign DNA to plasmids or bacteriophages. (correct)
- By enabling foreign DNA to integrate into chromosomal DNA.
- By promoting the cloning of only non-recombinant DNA.
- By reducing the copy number of foreign DNA in the cell.
What happens to an alien piece of DNA when it is linked with plasmid or bacteriophage DNA?
What happens to an alien piece of DNA when it is linked with plasmid or bacteriophage DNA?
What is the primary advantage of using modern cloning vectors?
What is the primary advantage of using modern cloning vectors?
Flashcards
Plasmid
Plasmid
A small circular piece of DNA that can replicate independently of the bacterial chromosome.
Bacteriophage
Bacteriophage
A virus that infects bacteria and can be used to deliver foreign DNA into bacterial cells.
Copy Number
Copy Number
The number of copies of a specific DNA molecule within a cell.
Cloning
Cloning
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Cloning Vector
Cloning Vector
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Study Notes
Cloning Vectors
- Plasmids and bacteriophages can replicate within bacterial cells independently of the cellular DNA.
- Bacteriophages have high copy numbers due to their high number per cell.
- Some plasmids have only one or two copies per cell, while others have 15-100 copies.
- Copy numbers can exceed 100.
- Linking foreign DNA to bacteriophage or plasmid DNA allows for replication of the foreign DNA equal to the copy number of the plasmid or bacteriophage.
- Present-day vectors are engineered to facilitate easy integration of foreign DNA and selection of recombinant cells over non-recombinant cells.
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