17 Questions
What is the primary function of a cloning vector?
To replicate itself within a host system
Which of the following is a characteristic of a suitable cloning vector?
All of the above
Why are bacterial plasmids commonly used as cloning vectors?
They are easily obtained, manipulated, and multiply rapidly within bacterial cells
What is the purpose of a selectable marker in a cloning vector?
To facilitate the selection of transformed cells
What is the process of cloning in biotechnology?
Creating exact copies of DNA fragments, cells, or organisms
Which type of cloning vector is capable of carrying larger DNA sequences than plasmid vectors?
Lamda phage vectors
What method is used to produce large amounts of DNA for study?
Vector delivery of DNA into host cell for amplification
Which type of cloning vector includes regulatory sequences necessary for the transcription and translation of the gene?
Expression vectors
Which technique is used to separate a mixture of nucleic acids based on size?
Gel electrophoresis
Which type of cloning vector can be packed into the phage body for transmission and use plasmid genes to direct its replication within the host cell?
Cosmids
Which method is used to monitor the success of a reaction involving restriction enzymes?
Gel electrophoresis
Who is credited with conducting the first gene splicing experiment?
Paul Berg
What is the purpose of using a plasmid like pSC101 in genetic engineering?
To confer Tetracycline resistance
What was the major contribution of Herbert Boyer to the field of genetic engineering?
Generating the first recombinant organism
Which technique is used to purify DNA using alcohol?
Freeze & crush
Which of the following is a challenge in manipulating DNA in the lab?
All of the above
What is the role of enzymes in the process of genetic engineering?
To cut and rejoin DNA fragments that contain genes
Learn about the selection and characteristics of cloning vectors in genetic engineering. Understand the importance of selectable markers and unique restriction enzyme recognition sites in cloning vectors.
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