Podcast
Questions and Answers
What is the primary function of a cloning vector?
What is the primary function of a cloning vector?
- To create genetic diversity within a host system
- To express the gene of interest inside a host system
- To amplify the gene of interest within a host system
- To replicate itself within a host system (correct)
Which of the following is a characteristic of a suitable cloning vector?
Which of the following is a characteristic of a suitable cloning vector?
- Presence of selectable markers
- Unique restriction enzyme recognition sites
- Ability to replicate independently
- All of the above (correct)
Why are bacterial plasmids commonly used as cloning vectors?
Why are bacterial plasmids commonly used as cloning vectors?
- They are easily obtained, manipulated, and multiply rapidly within bacterial cells (correct)
- They are difficult to obtain and manipulate
- They multiply slowly within bacterial cells
- They are not widely used in genetic engineering
What is the purpose of a selectable marker in a cloning vector?
What is the purpose of a selectable marker in a cloning vector?
What is the process of cloning in biotechnology?
What is the process of cloning in biotechnology?
Which type of cloning vector is capable of carrying larger DNA sequences than plasmid vectors?
Which type of cloning vector is capable of carrying larger DNA sequences than plasmid vectors?
What method is used to produce large amounts of DNA for study?
What method is used to produce large amounts of DNA for study?
Which type of cloning vector includes regulatory sequences necessary for the transcription and translation of the gene?
Which type of cloning vector includes regulatory sequences necessary for the transcription and translation of the gene?
Which technique is used to separate a mixture of nucleic acids based on size?
Which technique is used to separate a mixture of nucleic acids based on size?
Which type of cloning vector can be packed into the phage body for transmission and use plasmid genes to direct its replication within the host cell?
Which type of cloning vector can be packed into the phage body for transmission and use plasmid genes to direct its replication within the host cell?
Which method is used to monitor the success of a reaction involving restriction enzymes?
Which method is used to monitor the success of a reaction involving restriction enzymes?
Who is credited with conducting the first gene splicing experiment?
Who is credited with conducting the first gene splicing experiment?
What is the purpose of using a plasmid like pSC101 in genetic engineering?
What is the purpose of using a plasmid like pSC101 in genetic engineering?
What was the major contribution of Herbert Boyer to the field of genetic engineering?
What was the major contribution of Herbert Boyer to the field of genetic engineering?
Which technique is used to purify DNA using alcohol?
Which technique is used to purify DNA using alcohol?
Which of the following is a challenge in manipulating DNA in the lab?
Which of the following is a challenge in manipulating DNA in the lab?
What is the role of enzymes in the process of genetic engineering?
What is the role of enzymes in the process of genetic engineering?
