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Cloning Vectors in Genetic Engineering

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Questions and Answers

What is the primary function of a cloning vector?

To create genetic diversity within a host system

To express the gene of interest inside a host system

To amplify the gene of interest within a host system

To replicate itself within a host system (correct)

Which of the following is a characteristic of a suitable cloning vector?

Presence of selectable markers

Unique restriction enzyme recognition sites

Ability to replicate independently

All of the above (correct)

Why are bacterial plasmids commonly used as cloning vectors?

They are easily obtained, manipulated, and multiply rapidly within bacterial cells (correct)

They are difficult to obtain and manipulate

They multiply slowly within bacterial cells

They are not widely used in genetic engineering

What is the purpose of a selectable marker in a cloning vector?

To facilitate the selection of transformed cells Signup and view all the answers

What is the process of cloning in biotechnology?

Creating exact copies of DNA fragments, cells, or organisms Signup and view all the answers

Which type of cloning vector is capable of carrying larger DNA sequences than plasmid vectors?

Lamda phage vectors Signup and view all the answers

What method is used to produce large amounts of DNA for study?

Vector delivery of DNA into host cell for amplification Signup and view all the answers

Which type of cloning vector includes regulatory sequences necessary for the transcription and translation of the gene?

Expression vectors Signup and view all the answers

Which technique is used to separate a mixture of nucleic acids based on size?

Gel electrophoresis Signup and view all the answers

Which type of cloning vector can be packed into the phage body for transmission and use plasmid genes to direct its replication within the host cell?

Cosmids Signup and view all the answers

Which method is used to monitor the success of a reaction involving restriction enzymes?

Gel electrophoresis Signup and view all the answers

Who is credited with conducting the first gene splicing experiment?

Paul Berg Signup and view all the answers

What is the purpose of using a plasmid like pSC101 in genetic engineering?

To confer Tetracycline resistance Signup and view all the answers

What was the major contribution of Herbert Boyer to the field of genetic engineering?

Generating the first recombinant organism Signup and view all the answers

Which technique is used to purify DNA using alcohol?

Freeze & crush Signup and view all the answers

Which of the following is a challenge in manipulating DNA in the lab?

All of the above Signup and view all the answers

What is the role of enzymes in the process of genetic engineering?

To cut and rejoin DNA fragments that contain genes Signup and view all the answers