Clinical Bacteriology Prelims - Bacterial Genetics
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Questions and Answers

DNA segments that can move within the genome are known as ______.

transposons

The process by which bacteriophages may integrate into a bacterial host's chromosome is known as ______ conversion.

lysogenic

Mutations can occur due to exposure to certain chemical and physical ______.

agents

A change in the DNA base sequence is referred to as a ______.

<p>mutation</p> Signup and view all the answers

Bacteriophages that do not immediately kill their host are often involved in ______ cycles.

<p>lysogenic</p> Signup and view all the answers

The bacterial DNA carried by a bacteriophage enhances its ability to ______ genetic material.

<p>transfer</p> Signup and view all the answers

The term ______ is used to describe the phenomenon where cells can become immune to reinfection by the same phage.

<p>lysogenic conversion</p> Signup and view all the answers

Mutations can result from errors during ______ replication.

<p>DNA</p> Signup and view all the answers

The donor strain (F+) possesses a fertility factor (F factor) on a ______ that carries the genes for conjugative transfer.

<p>plasmid</p> Signup and view all the answers

Bacteriophage are ______ that consist of a chromosome surrounded by a protein coat.

<p>viruses</p> Signup and view all the answers

The ______ or virulent phage replicates usually through the lytic cycle.

<p>lytic</p> Signup and view all the answers

The production of viruses using a host cell to manufacture more viruses would cause the ______ bacteria to die.

<p>host</p> Signup and view all the answers

Bacteriophage DNA directs the bacterial cell to synthesize phage ______ and phage protein.

<p>DNA</p> Signup and view all the answers

In the lytic phase, the bacterial cell eventually ______, releasing a new phage.

<p>lyses</p> Signup and view all the answers

Temperate phage can replicate through the ______ cycle.

<p>lysogenic</p> Signup and view all the answers

The incorporation of the viral genome into the host cell genome creates a ______.

<p>prophage</p> Signup and view all the answers

The donor strain produces a hollow surface appendage called a ______ or conjugation pilus.

<p>sex pilus</p> Signup and view all the answers

Resistance plasmid conjugation involves sharing of the ______ which provides some form of antibiotic resistance.

<p>R plasmid</p> Signup and view all the answers

Transformation occurs after the uptake of DNA fragments from the ______

<p>environment</p> Signup and view all the answers

The chromosome is a single, long piece of circular, double-stranded ______

<p>DNA</p> Signup and view all the answers

Bacterial DNA contains 2000 to 4000 ______

<p>genes</p> Signup and view all the answers

Plasmids replicate ______

<p>independently</p> Signup and view all the answers

The RecA protein is a recombinase functioning in a recombinational DNA ______ in bacteria

<p>repair</p> Signup and view all the answers

Vertical gene transfer occurs from mother to cell ______

<p>offspring</p> Signup and view all the answers

Horizontal gene transfer includes processes such as ______ and transduction

<p>conjugation</p> Signup and view all the answers

Plasmids can contain genes for toxins and proteins that promote ______

<p>transfer</p> Signup and view all the answers

Competent bacteria are able to accept naked DNA from the ______

<p>environment</p> Signup and view all the answers

The number of plasmids in a bacterial cell may range from one to ______

<p>hundreds</p> Signup and view all the answers

Study Notes

Bacterial Genetics Overview

  • Bacterial transformation involves the uptake of DNA fragments from the environment.
  • The bacterial chromosome consists of a single, circular, double-stranded DNA with 2000 to 4000 genes essential for growth and replication.

Plasmids

  • Plasmids are small, circular DNA that replicate independently and contain genes for toxins and proteins promoting gene transfer.
  • They are not essential for growth, allowing bacteria to gain or lose them.
  • Plasmids can range from one to hundreds in a bacterial cell and may be exchanged nonsexually between species.

Gene Transfer Mechanisms

  • Vertical Gene Transfer occurs through binary fission from mother to offspring.
  • Horizontal Gene Transfer includes:
    • Conjugation: DNA transfer from a donor (F+) strain to a recipient via a sex pilus. The donor carries the F factor on a plasmid.
    • Transduction: Involves bacteriophages transferring DNA fragments between bacteria, with phages having either a lytic or lysogenic cycle.
      • Lytic Cycle: Destroys host bacteria, producing new phage particles that infect other bacteria.
      • Lysogenic Cycle: Integrates viral DNA into the host genome, creating a prophage without immediately killing the host.

Bacteriophage Types

  • Lytic or Virulent Phage: Causes host bacterial cell lysis, generating new viruses.
  • Temperate Phage: Integrates into the host genome and can remain dormant as a prophage.

Transposons

  • Transposons are mobile DNA segments capable of integrating into chromosomal DNA and facilitating the spread of antibiotic resistance genes.

Mutation

  • Mutations are changes in the DNA sequence that may alter protein coding, arising from single nucleotide changes or errors during replication at a rate of 1 in 10 million cells.
  • Physical and chemical agents can significantly elevate mutation rates.

Mobile Genetic Elements

  • Mobile genetic elements, also known as "jumping genes," can relocate within the chromosome, influencing genetic variability and evolution.

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Description

This quiz focuses on the principles of clinical bacteriology with a specific emphasis on bacterial genetics, particularly the process of transformation. Ideal for 1st semester students preparing for prelims, it covers key concepts related to DNA uptake and chromosome structure in bacteria.

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