Bacterial Genetics: Conjugation, Transformation

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Questions and Answers

A researcher is studying a bacterial population and observes that some bacteria are incorporating new genetic material from their surroundings. Which mechanism of genetic transfer is most likely responsible for this?

  • Transformation, the uptake of free DNA from the environment. (correct)
  • Conjugation, which involves direct contact between bacterial cells.
  • Transposition, where mobile genetic elements move within a genome.
  • Transduction, mediated by bacteriophages transferring genetic material.

A scientist discovers a new bacterial strain that exhibits unusually high antibiotic resistance. Further analysis reveals that the resistance genes are located on a plasmid. Which process could have most likely introduced these genes into the bacterial strain?

  • Transformation, which requires a bacteriophage to transfer DNA.
  • Mutation, a spontaneous change in the bacterial chromosome.
  • Transposition, involving the movement of DNA segments within the same cell.
  • Conjugation, the transfer of genetic material between bacterial cells via direct contact. (correct)

In a laboratory experiment, bacterial cells are infected with a bacteriophage. After lysis, the newly released phages transfer genetic material from the first bacterial cells to a second population of bacterial cells. What is this process called?

  • Transduction (correct)
  • Transformation
  • Transposition
  • Conjugation

Mobile genetic elements known as transposons are capable of moving from one location to another within a bacterial chromosome or even to a plasmid. What is the process called?

<p>Transposition, the movement of genetic elements within a genome. (D)</p> Signup and view all the answers

Which of the following mechanisms of genetic transfer in bacteria requires direct physical contact between the donor and recipient cells?

<p>Conjugation (A)</p> Signup and view all the answers

A bacterial cell acquires resistance to an antibiotic after incorporating a DNA fragment from its environment. This fragment originated from a dead bacterium. Which mechanism of genetic transfer explains this phenomenon?

<p>Transformation, the direct uptake of genetic material from the environment. (B)</p> Signup and view all the answers

A scientist is studying a bacterial population and notices genetic changes occurring after infection with a bacteriophage. The changes involve the transfer of DNA from one bacterium to another via the phage. What is the name of this mechanism?

<p>Transduction (D)</p> Signup and view all the answers

Which process involves the movement of a mobile genetic element to various locations within the bacterial genome, potentially causing mutations or changes in gene expression?

<p>Transposition, the movement of genetic elements within a genome. (D)</p> Signup and view all the answers

A researcher isolates a bacterial strain that contains a new plasmid. Upon further investigation, it is discovered that the plasmid was transferred from another bacterium through direct contact. What mechanism of genetic transfer is responsible for this observation?

<p>Conjugation (D)</p> Signup and view all the answers

How does transduction differ fundamentally from transformation and conjugation in bacterial genetics?

<p>Transduction is mediated by bacteriophages, while transformation involves direct DNA uptake and conjugation requires cell contact. (C)</p> Signup and view all the answers

Flashcards

Conjugation

The transfer of genetic material between bacterial cells by direct cell-to-cell contact or through a bridge-like connection.

Transformation

The genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (DNA) from its surroundings through the cell membrane(s).

Transduction

The process by which foreign DNA is introduced into a cell by a virus or viral vector.

Transposition

A genetic element that can move (transpose) to different locations within a genome.

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Bacterial Genetics

The study of genes, genetic variation, and heredity in bacteria.

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Study Notes

  • Professor Zach Murphy will lecture on bacterial genetics.
  • The lecture covers conjugation, transformation, transduction, and transposition.

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