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Questions and Answers
What is the main function of restriction enzymes in bacteria and archaea?
What is the main function of restriction enzymes in bacteria and archaea?
- To aid in the replication of host DNA
- To modify the structure of prokaryotic DNA
- To provide a defense mechanism against invading viruses (correct)
- To enhance the process of molecular cloning
How do restriction enzymes cut DNA?
How do restriction enzymes cut DNA?
- By introducing mutations at specific recognition sites
- By breaking the hydrogen bonds between base pairs
- By unwinding the DNA double helix
- By making two incisions, once through each sugar-phosphate backbone of the DNA double helix (correct)
What is the role of modification enzymes in the restriction modification system?
What is the role of modification enzymes in the restriction modification system?
- To enhance the activity of restriction enzymes
- To facilitate the replication of host DNA
- To modify the prokaryotic DNA and protect it from cleavage (correct)
- To degrade foreign DNA
How many different specificities do the known restriction endonucleases represent?
How many different specificities do the known restriction endonucleases represent?
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What are restriction enzymes routinely used for in laboratories?
What are restriction enzymes routinely used for in laboratories?
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