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Questions and Answers
Which group of bacteria is known for having no peptidoglycan in their cell walls?
All Prokaryotes are eukaryotic organisms.
False
What is the primary method of reproduction in Eubacteria?
Binary fission
Methanogens are examples of ______ that thrive in extreme living conditions.
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Match the following groups of bacteria with their respective volumes:
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Extreme halophiles are known to cause disease in humans.
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Which of the following groups is characterized by a lack of peptidoglycan?
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What is the primary purpose of the 2nd edition mentioned in the content?
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The genus ______ includes species that share common characteristics and are closely related.
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Which of the following is a characteristic of methanogens?
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Match the following kingdoms with their respective domains:
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What is an example of a species under the genus Lactobacillus?
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All prokaryotic organisms are classified under the same domain.
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What is the term for the classification system introduced by the 'Father of Modern Taxonomy'?
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The species name is capitalized in the binomial naming system.
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What should be done to the names of all taxa in scientific writing?
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The genus name and species name together are known as _____ names.
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Match the following microorganism names with their descriptions:
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Which of the following statements is true regarding the publication 'Systema Naturae'?
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All strain designations and numbers are italicized in scientific writing.
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A specific epithet must be preceded by a _____ name.
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What is the primary focus of Bergey’s Manual of Systematic Bacteriology?
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The Kingdom designation is widely accepted in microbial phylogeny.
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What did David H. Bergey contribute to microbiology in 1923?
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The __________ domain includes unicellular prokaryotes with a cell wall containing peptidoglycan.
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Which of the following is true about the Domain Archaea?
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Match each manual to its publication year:
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Each domain is divided into __________, which are then divided into classes.
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Sponges, worms, insects, and chordates are examples of prokaryotic organisms.
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How is E.coli O157 correctly written?
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Gram staining is a biochemical characteristic used for the identification of microorganisms.
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What is slide agglutination commonly used for?
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_________ include characteristics such as size, shape, and cellular structures.
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Match the following identification methods with their descriptions:
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Which of the following is NOT a biochemical characteristic?
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Cell wall structure can be determined through DNA hybridization.
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What is one advantage of DNA chip technology in microorganism identification?
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Study Notes
Classification of Organisms
- Classification relies on phylogenetic relationships, mainly through nucleic acid sequences like 16S rRNA.
- Main kingdoms: Prokaryotae (Monera), Protista, Fungi, Plantae, and Animalia.
Prokaryotic Kingdoms
- Eubacteria: True bacteria that reproduce via binary fission; gain energy from organic/inorganic chemicals or photosynthesis.
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Archaebacteria (Archaea):
- Exhibit unusual metabolism.
- Thrive in extreme environments; lack peptidoglycan in cell walls.
- Not known to cause diseases in humans and animals, examples include Methanogens, Halophiles, and Thermoacidophiles.
Bergey's Manuals
- Bergey's Manual of Systematic Bacteriology: A comprehensive classification effort begun in 2001 to update knowledge on prokaryotic organisms.
- Second edition will consist of five volumes, covering different bacterial and archaeal groups:
- Volume I: Archaea and deeply branching bacteria.
- Volume II: Proteobacteria.
- Volume III: Firmicutes.
- Volume IV: Spirochaetes, Bacteroidetes, others.
- Volume V: Actinobacteria.
Domain Eukarya
- Encompasses all eukaryotic organisms, including:
- Kingdom Animalia
- Kingdom Plantae
- Kingdom Fungi
- Kingdom Protista
Taxonomic Hierarchy
- Taxonomic categories include Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species, and Subspecies.
- Genus represents closely related species, with scientific names using binomial nomenclature (e.g., Bacillus anthracis).
Naming Conventions
- Genus names are capitalized; species names are lowercase and italicized.
- Full genus name must be included for specific epithets upon first use.
- Unique morphology, ecological traits, and biochemical characteristics aid in identification.
Microbial Identification Techniques
- Classification involves morphological characteristics, DNA hybridization, and rRNA sequencing.
- Identification combines various methods including serology (study of immune responses).
- Techniques include slide agglutination, ELISA, Western blotting, Southern blotting, and DNA chips.
Classification Properties
- Key attributes for classification:
- Colony morphology and cell shape/arrangement.
- Cell wall structure (e.g., Gram staining) and special cellular structures.
- Biochemical characteristics enhance identification accuracy and specificity.
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Description
Test your knowledge on the classification of prokaryotes, focusing on Eubacteria and Archaebacteria. This quiz covers phylogenetic relationships and the use of nucleic acid sequences like 16S rRNA. Challenge yourself with questions about the characteristics and energy sources of different bacteria.