Podcast
Questions and Answers
What pH value is associated with the prepared Citrate buffer before any adjustments are made?
What pH value is associated with the prepared Citrate buffer before any adjustments are made?
- 4.8 (correct)
- 5.5
- 2.0
- 3.0
How much sodium citrate solution is needed to prepare the 0.1M Citrate buffer?
How much sodium citrate solution is needed to prepare the 0.1M Citrate buffer?
- 5 ml
- 2.941 gm
- 2.101 gm
- 3.5 ml (correct)
Which of the following steps is NOT part of preparing the Citrate buffer solution?
Which of the following steps is NOT part of preparing the Citrate buffer solution?
- Adding sodium citrate first to the acid (correct)
- Adjust the pH with hydrochloric acid and sodium hydroxide
- Dissolve 2.941 gm of sodium citrate in 100 ml distilled water
- Dissolve 2.101 gm of citric acid in 100 ml distilled water
What is the mass of citric acid required for preparing the Citrate buffer?
What is the mass of citric acid required for preparing the Citrate buffer?
In preparing the Carbonate - Bicarbonate buffer, how much anhydrous sodium carbonate is required for a 0.2M solution?
In preparing the Carbonate - Bicarbonate buffer, how much anhydrous sodium carbonate is required for a 0.2M solution?
What is the final pH of the citrate buffer after adjustments?
What is the final pH of the citrate buffer after adjustments?
Which reagent is mixed with citric acid to create the citrate buffer?
Which reagent is mixed with citric acid to create the citrate buffer?
How is the concentration of the citrate buffer described in the procedure?
How is the concentration of the citrate buffer described in the procedure?
To achieve a pH of 2.5, which two solutions are used for adjustment?
To achieve a pH of 2.5, which two solutions are used for adjustment?
What is the initial preparation step for the sodium carbonate solution in the carbonate-bicarbonate buffer?
What is the initial preparation step for the sodium carbonate solution in the carbonate-bicarbonate buffer?
Study Notes
Citrate Buffer
- Reagents Needed:
- Citric acid: 2.101g dissolved in 100ml distilled water.
- Sodium citrate solution (0.1M): 2.941g dissolved in 100ml distilled water.
- Preparation Procedure:
- Mix 46.5ml of citric acid with 3.5ml of sodium citrate solution.
- Add distilled water to reach a total volume of 100ml.
- This creates a 0.1M citrate buffer.
- pH Measurement:
- Standardize the solution using a pH meter to measure the pH.
- Initial pH was measured at 4.8.
- Adjust the pH to the desired 2.5 using IN HCl and 5N NaOH.
Carbonate-Bicarbonate Buffer
- Reagents Needed:
- Sodium carbonate solution (0.2M): 2.12g of anhydrous sodium carbonate dissolved in 100ml distilled water.
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Description
Test your knowledge on preparing a citrate buffer using citric acid and sodium citrate. This quiz will cover the reagents required, the procedure for preparation, and the importance of pH measurement. Perfect for students learning about buffer solutions in chemistry!
