20 Questions
Which part of a chromosome is labeled 'p'?
Short arm
What is the primary function of colchicine in chromosome preparation?
Stop cell division at metaphase
What characterizes the banding patterns of chromosomes?
Consistent among species
In chromosome analysis, what is the significance of polimorfik bölgeler?
Represent structural variations
What is the main objective of chromosomal bantlama/boyama in karyotype analysis?
Detect chromosomal abnormalities
What is the purpose of using FISH with dual color and dual transfusion probes for BCR and ABL1?
Detecting translocation between chromosomes 9 and 22
In FISH analysis, a normal cell would typically show how many sets of red and green signals?
2R2G
Which of the following is NOT an example where FISH method can be applied according to the provided text?
Investigating the structure of proteins
What does a cell with a t(9;22) translocation exhibit in terms of BCR (green) and ABL1 (red) signals?
Individual signals from chromosomes 9 and 22 with no fusion signals
Why can formalin-fixed tissue samples be used for FISH analysis even after months or years?
Because formalin preserves DNA integrity
Which technique involves treating chromosomes first with acid and then with alkali before staining with Giemsa dye?
G-banding
Which staining technique specifically targets the nucleolus organizer regions in the satellite regions of acrocentric chromosomes?
NOR staining
What type of genetic exchange occurs during sister chromatid exchange (SCE)?
Symmetric exchange of genetic material between identical segments
Which technique allows the researcher to separate the chromatids during two cell cycles by incorporating BrdU in the culture medium?
Sister chromatid banding
Which characteristic of heterochromatin regions makes them appear light or matte in C-banding or R-banding techniques?
Rich in A-T base pairs
What is the primary purpose of creating regions on chromosomes during physical mapping?
To identify specific genes responsible for diseases or traits
How does the technique of G-banding assist in cytogenetic analysis?
By staining chromosomes to create unique banding patterns for each chromosome
What improves as the number of subregions within a chromosome region increases?
The resolution for pinpointing gene locations improves
In genetic mapping, what does the term 'localization' refer to?
Determining the physical position of a gene or DNA sequence on a chromosome
What does G-banding help reveal about chromosomes?
Their unique banding patterns that aid in identification
Learn about the methods used to create regions on chromosomes and how they are named. Explore how chromosomes are divided into short and long arms, and further subdivided into upper and lower regions.
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