Chromosome Mapping Techniques

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Questions and Answers

Which part of a chromosome is labeled 'p'?

  • Banding region
  • Centromere
  • Short arm (correct)
  • Long arm

What is the primary function of colchicine in chromosome preparation?

  • Stop cell division at metaphase (correct)
  • Prevent DNA degradation
  • Enhance chromosomal banding
  • Induce polyploidy

What characterizes the banding patterns of chromosomes?

  • Unique in each individual
  • Dependent on environmental factors
  • Dynamic during cell division
  • Consistent among species (correct)

In chromosome analysis, what is the significance of polimorfik bölgeler?

<p>Represent structural variations (D)</p> Signup and view all the answers

What is the main objective of chromosomal bantlama/boyama in karyotype analysis?

<p>Detect chromosomal abnormalities (A)</p> Signup and view all the answers

What is the purpose of using FISH with dual color and dual transfusion probes for BCR and ABL1?

<p>Detecting translocation between chromosomes 9 and 22 (D)</p> Signup and view all the answers

In FISH analysis, a normal cell would typically show how many sets of red and green signals?

<p>2R2G (A)</p> Signup and view all the answers

Which of the following is NOT an example where FISH method can be applied according to the provided text?

<p>Investigating the structure of proteins (D)</p> Signup and view all the answers

What does a cell with a t(9;22) translocation exhibit in terms of BCR (green) and ABL1 (red) signals?

<p>Individual signals from chromosomes 9 and 22 with no fusion signals (C)</p> Signup and view all the answers

Why can formalin-fixed tissue samples be used for FISH analysis even after months or years?

<p>Because formalin preserves DNA integrity (B)</p> Signup and view all the answers

Which technique involves treating chromosomes first with acid and then with alkali before staining with Giemsa dye?

<p>G-banding (A)</p> Signup and view all the answers

Which staining technique specifically targets the nucleolus organizer regions in the satellite regions of acrocentric chromosomes?

<p>NOR staining (D)</p> Signup and view all the answers

What type of genetic exchange occurs during sister chromatid exchange (SCE)?

<p>Symmetric exchange of genetic material between identical segments (D)</p> Signup and view all the answers

Which technique allows the researcher to separate the chromatids during two cell cycles by incorporating BrdU in the culture medium?

<p>Sister chromatid banding (C)</p> Signup and view all the answers

Which characteristic of heterochromatin regions makes them appear light or matte in C-banding or R-banding techniques?

<p>Rich in A-T base pairs (A)</p> Signup and view all the answers

What is the primary purpose of creating regions on chromosomes during physical mapping?

<p>To identify specific genes responsible for diseases or traits (C)</p> Signup and view all the answers

How does the technique of G-banding assist in cytogenetic analysis?

<p>By staining chromosomes to create unique banding patterns for each chromosome (D)</p> Signup and view all the answers

What improves as the number of subregions within a chromosome region increases?

<p>The resolution for pinpointing gene locations improves (B)</p> Signup and view all the answers

In genetic mapping, what does the term 'localization' refer to?

<p>Determining the physical position of a gene or DNA sequence on a chromosome (C)</p> Signup and view all the answers

What does G-banding help reveal about chromosomes?

<p>Their unique banding patterns that aid in identification (A)</p> Signup and view all the answers

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