Chromosome Mapping Techniques
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Questions and Answers

Which part of a chromosome is labeled 'p'?

  • Banding region
  • Centromere
  • Short arm (correct)
  • Long arm
  • What is the primary function of colchicine in chromosome preparation?

  • Stop cell division at metaphase (correct)
  • Prevent DNA degradation
  • Enhance chromosomal banding
  • Induce polyploidy
  • What characterizes the banding patterns of chromosomes?

  • Unique in each individual
  • Dependent on environmental factors
  • Dynamic during cell division
  • Consistent among species (correct)
  • In chromosome analysis, what is the significance of polimorfik bölgeler?

    <p>Represent structural variations</p> Signup and view all the answers

    What is the main objective of chromosomal bantlama/boyama in karyotype analysis?

    <p>Detect chromosomal abnormalities</p> Signup and view all the answers

    What is the purpose of using FISH with dual color and dual transfusion probes for BCR and ABL1?

    <p>Detecting translocation between chromosomes 9 and 22</p> Signup and view all the answers

    In FISH analysis, a normal cell would typically show how many sets of red and green signals?

    <p>2R2G</p> Signup and view all the answers

    Which of the following is NOT an example where FISH method can be applied according to the provided text?

    <p>Investigating the structure of proteins</p> Signup and view all the answers

    What does a cell with a t(9;22) translocation exhibit in terms of BCR (green) and ABL1 (red) signals?

    <p>Individual signals from chromosomes 9 and 22 with no fusion signals</p> Signup and view all the answers

    Why can formalin-fixed tissue samples be used for FISH analysis even after months or years?

    <p>Because formalin preserves DNA integrity</p> Signup and view all the answers

    Which technique involves treating chromosomes first with acid and then with alkali before staining with Giemsa dye?

    <p>G-banding</p> Signup and view all the answers

    Which staining technique specifically targets the nucleolus organizer regions in the satellite regions of acrocentric chromosomes?

    <p>NOR staining</p> Signup and view all the answers

    What type of genetic exchange occurs during sister chromatid exchange (SCE)?

    <p>Symmetric exchange of genetic material between identical segments</p> Signup and view all the answers

    Which technique allows the researcher to separate the chromatids during two cell cycles by incorporating BrdU in the culture medium?

    <p>Sister chromatid banding</p> Signup and view all the answers

    Which characteristic of heterochromatin regions makes them appear light or matte in C-banding or R-banding techniques?

    <p>Rich in A-T base pairs</p> Signup and view all the answers

    What is the primary purpose of creating regions on chromosomes during physical mapping?

    <p>To identify specific genes responsible for diseases or traits</p> Signup and view all the answers

    How does the technique of G-banding assist in cytogenetic analysis?

    <p>By staining chromosomes to create unique banding patterns for each chromosome</p> Signup and view all the answers

    What improves as the number of subregions within a chromosome region increases?

    <p>The resolution for pinpointing gene locations improves</p> Signup and view all the answers

    In genetic mapping, what does the term 'localization' refer to?

    <p>Determining the physical position of a gene or DNA sequence on a chromosome</p> Signup and view all the answers

    What does G-banding help reveal about chromosomes?

    <p>Their unique banding patterns that aid in identification</p> Signup and view all the answers

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