10 Questions
Which of the following best describes the purpose of preparative chromatography?
To separate a mixture into its components
What is the mobile phase in chromatography?
The fluid solvent that carries the mixture
What is the stationary phase in chromatography?
The material fixed on the system
What causes the separation of components in chromatography?
Different affinities for the stationary phase
What is the basis of separation in chromatography?
Differential partitioning between the mobile and stationary phases
Which of the following best describes the purpose of preparative chromatography?
To separate a mixture into its components
What is the basis of separation in chromatography?
Differential partitioning
What is the mobile phase in chromatography?
The fluid solvent that carries the mixture
What is the stationary phase in chromatography?
The material fixed on the system
What causes the separation of components in chromatography?
Different affinities for the stationary phase
Study Notes
Chromatography Basics
- The purpose of preparative chromatography is to isolate and purify a specific compound or a mixture of compounds on a large scale, often for further use or analysis.
Chromatography Components
- The mobile phase in chromatography is the solvent or mixture of solvents that flows through the system, carrying the sample with it.
- The stationary phase in chromatography is the solid or liquid material that is stationary in the system, and interacts with the sample to separate its components.
Chromatography Separation
- The separation of components in chromatography is caused by the different interactions between the sample components and the stationary phase, resulting in different rates of migration through the system.
- The basis of separation in chromatography is the varying affinity of the sample components for the stationary phase, which affects their migration rates and ultimately results in their separation.
Test your knowledge of chromatography with this quiz! Learn about the principles and techniques involved in separating mixtures using the mobile and stationary phases. Discover how chromatography is used in chemical analysis and explore its applications in various industries.
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