Chromatography Principles Quiz
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Questions and Answers

What is the main principle used in TLC?

  • Gravity
  • Adsorption (correct)
  • Diffusion
  • Osmosis

Which type of interactions play a key role in TLC for polar molecules?

  • Covalent bonding
  • Hydrogen bonding
  • Dipole-dipole interactions (correct)
  • Ionic bonding

What determines the movement of a substance in TLC?

  • Its shape
  • Its interaction with the stationary phase (correct)
  • Its color intensity
  • Its molecular weight

What does Rf refer to in TLC?

<p>Retention or retardation factor (A)</p> Signup and view all the answers

Which factor causes substances to separate on the stationary phase in TLC?

<p>Their affinity towards the stationary phase (B)</p> Signup and view all the answers

What is represented by the Rf value in TLC?

<p>The distance traveled by the solute relative to the solvent front (A)</p> Signup and view all the answers

What is the advantage of using sulfuric acid/heat to visualize compounds in TLC?

<p>Leaves charred blots behind (C)</p> Signup and view all the answers

Which step is critical in running a TLC to avoid immersion of the spotting area in the mobile phase?

<p>Spotting the sample using a capillary tube or micropipette (C)</p> Signup and view all the answers

Why is ninhydrin reagent useful in TLC analysis?

<p>Reacts with amino acids to reveal fingerprints (A)</p> Signup and view all the answers

What characterizes the UV light technique for visualizing compounds in TLC?

<p>Long wavelength results in compounds glowing (D)</p> Signup and view all the answers

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