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Chromatography Principles Quiz

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Questions and Answers

What is the main principle used in TLC?

Gravity

Adsorption (correct)

Diffusion

Osmosis

Which type of interactions play a key role in TLC for polar molecules?

Covalent bonding

Hydrogen bonding

Dipole-dipole interactions (correct)

Ionic bonding

What determines the movement of a substance in TLC?

Its shape

Its interaction with the stationary phase (correct)

Its color intensity

Its molecular weight

What does Rf refer to in TLC?

Retention or retardation factor Signup and view all the answers

Which factor causes substances to separate on the stationary phase in TLC?

Their affinity towards the stationary phase Signup and view all the answers

What is represented by the Rf value in TLC?

The distance traveled by the solute relative to the solvent front Signup and view all the answers

What is the advantage of using sulfuric acid/heat to visualize compounds in TLC?

Leaves charred blots behind Signup and view all the answers

Which step is critical in running a TLC to avoid immersion of the spotting area in the mobile phase?

Spotting the sample using a capillary tube or micropipette Signup and view all the answers

Why is ninhydrin reagent useful in TLC analysis?

Reacts with amino acids to reveal fingerprints Signup and view all the answers

What characterizes the UV light technique for visualizing compounds in TLC?

Long wavelength results in compounds glowing Signup and view all the answers