Questions and Answers
What is the main principle used in TLC?
Adsorption
Which type of interactions play a key role in TLC for polar molecules?
Dipole-dipole interactions
What determines the movement of a substance in TLC?
Its interaction with the stationary phase
What does Rf refer to in TLC?
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Which factor causes substances to separate on the stationary phase in TLC?
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What is represented by the Rf value in TLC?
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What is the advantage of using sulfuric acid/heat to visualize compounds in TLC?
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Which step is critical in running a TLC to avoid immersion of the spotting area in the mobile phase?
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Why is ninhydrin reagent useful in TLC analysis?
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What characterizes the UV light technique for visualizing compounds in TLC?
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