Podcast
Questions and Answers
What is the primary reason for tailing effects in chromatography?
What is the primary reason for tailing effects in chromatography?
- Insufficient number of adsorption sites
- Poor distribution coefficient
- Adsorption processes (correct)
- Lack of suitable mobile phase
What is the result of a stationary phase with insufficient adsorption sites?
What is the result of a stationary phase with insufficient adsorption sites?
- Coelution
- Poor resolution
- Tailing effect
- Fronting effect (correct)
What is the relationship between the retention factor k' and the analysis time?
What is the relationship between the retention factor k' and the analysis time?
- Lower k' values result in longer analysis times
- Higher k' values result in shorter analysis times
- Higher k' values result in longer analysis times and wider peaks (correct)
- Lower k' values result in higher sensitivity
What is the significance of a selectivity value of α = 1?
What is the significance of a selectivity value of α = 1?
What is the effect of a large retention factor k' on the peak shape and sensitivity?
What is the effect of a large retention factor k' on the peak shape and sensitivity?
What is the primary mechanism of interaction between the solute and the stationary phase in adsorption chromatography?
What is the primary mechanism of interaction between the solute and the stationary phase in adsorption chromatography?
Which type of chromatography is characterized by a solid stationary phase and a liquid or gas mobile phase?
Which type of chromatography is characterized by a solid stationary phase and a liquid or gas mobile phase?
What is the definition of the retention factor k' in chromatography?
What is the definition of the retention factor k' in chromatography?
What affects the movement of a solute through the column in adsorption chromatography?
What affects the movement of a solute through the column in adsorption chromatography?
Which of the following is a typical example of adsorption chromatography?
Which of the following is a typical example of adsorption chromatography?
What is the primary factor that influences the classification of chromatographic methods based on the mechanism of interaction?
What is the primary factor that influences the classification of chromatographic methods based on the mechanism of interaction?
In which type of chromatography does the solute interact with the stationary phase through electrostatic attraction?
In which type of chromatography does the solute interact with the stationary phase through electrostatic attraction?
What is the primary mechanism of attraction in ion-exchange chromatography?
What is the primary mechanism of attraction in ion-exchange chromatography?
What is the primary function of the stationary phase in partition chromatography?
What is the primary function of the stationary phase in partition chromatography?
What type of chromatography involves the separation of solutes based on their size?
What type of chromatography involves the separation of solutes based on their size?
What is the nature of the interaction between the solute and the stationary phase in molecular exclusion chromatography?
What is the nature of the interaction between the solute and the stationary phase in molecular exclusion chromatography?
What is the primary mechanism of solute separation in partition chromatography?
What is the primary mechanism of solute separation in partition chromatography?
What is the physical state of the stationary phase in gas chromatography?
What is the physical state of the stationary phase in gas chromatography?
