Chromatography Peak Shapes and Effects
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Questions and Answers

What is the primary reason for tailing effects in chromatography?

  • Insufficient number of adsorption sites
  • Poor distribution coefficient
  • Adsorption processes (correct)
  • Lack of suitable mobile phase

    • What is the result of a stationary phase with insufficient adsorption sites?

  • Coelution
  • Poor resolution
  • Tailing effect
  • Fronting effect (correct)

    • What is the relationship between the retention factor k' and the analysis time?

  • Lower k' values result in longer analysis times
  • Higher k' values result in shorter analysis times
  • Higher k' values result in longer analysis times and wider peaks (correct)
  • Lower k' values result in higher sensitivity

    • What is the significance of a selectivity value of α = 1?

    <p>Coelution occurs, no separation is possible</p> Signup and view all the answers

    What is the effect of a large retention factor k' on the peak shape and sensitivity?

    <p>Wider peaks and lower sensitivity</p> Signup and view all the answers

    What is the primary mechanism of interaction between the solute and the stationary phase in adsorption chromatography?

    <p>Adsorption on the surface of the solid particles</p> Signup and view all the answers

    Which type of chromatography is characterized by a solid stationary phase and a liquid or gas mobile phase?

    <p>Adsorption chromatography</p> Signup and view all the answers

    What is the definition of the retention factor k' in chromatography?

    <p>Product of the distribution coefficient and the ratio of the volume of the stationary phase to the mobile phase</p> Signup and view all the answers

    What affects the movement of a solute through the column in adsorption chromatography?

    <p>The strength of adsorption on the surface of the solid particles</p> Signup and view all the answers

    Which of the following is a typical example of adsorption chromatography?

    <p>Thin-layer chromatography (TLC)</p> Signup and view all the answers

    What is the primary factor that influences the classification of chromatographic methods based on the mechanism of interaction?

    <p>The mechanism of interaction of the solute with the stationary phase</p> Signup and view all the answers

    In which type of chromatography does the solute interact with the stationary phase through electrostatic attraction?

    <p>Ion-exchange chromatography</p> Signup and view all the answers

    What is the primary mechanism of attraction in ion-exchange chromatography?

    <p>Electrostatic force</p> Signup and view all the answers

    What is the primary function of the stationary phase in partition chromatography?

    <p>To provide a surface for solute equilibration</p> Signup and view all the answers

    What type of chromatography involves the separation of solutes based on their size?

    <p>Molecular exclusion chromatography</p> Signup and view all the answers

    What is the nature of the interaction between the solute and the stationary phase in molecular exclusion chromatography?

    <p>Size exclusion</p> Signup and view all the answers

    What is the primary mechanism of solute separation in partition chromatography?

    <p>Solute distribution between phases</p> Signup and view all the answers

    What is the physical state of the stationary phase in gas chromatography?

    <p>Liquid</p> Signup and view all the answers

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