Questions and Answers
Chromatographic theory A- Plate theory states that the column is divided into N segments, in each of which equilibrium occurs of a solute between ______ and stationary phases.
mobile
The height equivalent to a theoretical plate (H.E.T.P.) is measured by the total length of the column, L, divided by the number of ______.
segments
The width of a chromatographic peak, w, is equal to four standard deviations of a Gaussian peak (4σ), or if measured at half of the peak height, it is equal to ______.
2σ
Theoretical number of separation steps (plate), denoted as N, determines the ______ of a column.
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If N is constant, the width of a chromatographic peak increases with increasing ______ time.
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The van Deemter equation is used to account for the ______ of an elution band in chromatography.
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The number of theoretical plates (N) can be measured by analyzing the retention time (tR) or retention volume (VR) along with the ______.
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The width of chromatographic peak is directly related to the number of theoretical plates, with a higher N value resulting in a narrower ______.
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In chromatographic theory, a theoretical plate represents a segment of the column where equilibrium occurs between the mobile and stationary phases, acting as a fundamental unit for analyzing ______.
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The rate of solute migration through the column can be described by an equation that takes into account the number of theoretical ______.
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