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Chromatography: Distribution Constant K

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Questions and Answers

What is the distribution constant (K) in chromatography?

The ratio of solute concentration in the stationary phase to the mobile phase (correct)

The multiplication of solute concentrations in both stationary and mobile phases

The ratio of solute concentration in the mobile phase to the stationary phase

The sum of solute concentrations in both stationary and mobile phases

What does linear chromatography refer to?

Chromatography with a linear increase in retention time

Chromatographic separation obeying a specific distribution constant equation (correct)

Separation resulting in a Gaussian peak independent of sample amount

Chromatographic technique using a straight column

What does tR' represent in chromatography?

Time spent in the stationary phase by the analyte (correct)

Retention time of the mobile phase

Length of the column

Retention time of un-retained substance

How is the average linear velocity of solute (V') calculated?

<p>Column length divided by retention time of solute</p> Signup and view all the answers

What does U represent in terms of linear velocity in chromatography?

<p>Linear velocity of the mobile phase</p> Signup and view all the answers

Study Notes

What is the distribution constant (K) in chromatography? What does linear chromatography refer to? What does tR' represent in chromatography? How is the average linear velocity of solute (V') calculated? What does U represent in terms of linear velocity in chromatography?

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Description

Learn about the distribution constant K in chromatography, which represents the interaction of solutes with stationary and mobile phases. Understand how the concentrations of solute in both phases affect the separation process.