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Questions and Answers
What are the two main phases involved in chromatography?
What are the two main phases involved in chromatography?
- Adsorbent and solute
- Stationary phase and mobile phase (correct)
- Solid phase and liquid phase
- Gas phase and liquid phase
What determines how components of a sample separate during chromatography?
What determines how components of a sample separate during chromatography?
- Adsorption rate and solubility in the solvent (correct)
- Color and size of the components
- Volume of the mobile phase used
- Temperature of the solvent
In the chromatographic setup, what does the piece of paper represent?
In the chromatographic setup, what does the piece of paper represent?
- The sample solution
- The mobile phase
- The lost component
- The stationary phase (correct)
What is the role of the solvent in a chromatography process?
What is the role of the solvent in a chromatography process?
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What happens to the different components of a sample as the solvent moves up the paper?
What happens to the different components of a sample as the solvent moves up the paper?
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Study Notes
Chromatography
- Chromatography separates components based on their different adsorption rates AND solubility in the solvent.
- Separation relies on a stationary phase (adsorbent) and a mobile phase (solvent).
- Different components of a sample travel at different rates, resulting in visible separation.
- An example of a chromatographic setup uses paper as the stationary phase.
- A sample is spotted at the bottom of the paper and a solvent is drawn up, carrying different components at different rates.
- The different components form distinct spots, allowing identification and analysis.
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