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Chemistry: Chromatography Techniques

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Questions and Answers

What characterizes the stationary phase in chromatography?

It contains all the components to be separated.

It has a higher distribution constant than the mobile phase.

It remains fixed in place during the process. (correct)

It moves through the stationary phase.

In the context of chromatography, what does a higher distribution constant indicate for a component?

The component will not separate from others.

The component moves faster through the mobile phase.

The component is more likely to be found in the stationary phase. (correct)

The component mixes evenly with solvent.

Which statement describes the purpose of chromatography?

To increase the concentration of all components in a mixture.

To identify components without separating them.

To separate components of a mixture and identify them. (correct)

To chemically alter the components in a mixture.

How do molecules in chromatography determine their movement?

<p>By the time they spend in each phase.</p> Signup and view all the answers

What is the primary reason molecules separate during chromatography?

<p>Differences in their affinity for the stationary and mobile phases.</p> Signup and view all the answers

Study Notes

Chromatography Overview

Chromatography is a physical separation method involving distribution of components between two phases: stationary and mobile.
The stationary phase remains fixed in place, while the mobile phase moves in a definite direction.
Separation occurs due to differences in a distribution constant among the components within a mixture.

Mechanism of Action

The technique relies on the tendency of molecules to spend varying amounts of time in each phase.
Molecules that prefer the mobile phase move faster through the system, leading to separation.
This differential distribution allows for the identification and separation of individual components in a mixture.

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Description

Explore the fascinating world of chromatography, a physical method of separation where components of a mixture are distributed between stationary and moving phases. Understand how differences in distribution constants of the components aid in their separation and identification. This quiz will test your knowledge on various chromatography techniques and their applications.