COPY: Chromatography Basics Quiz
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Questions and Answers

What is the term for the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase?

  • Elution (correct)
  • Eluent
  • Chromatography
  • Affinity

    • In column chromatography, what determines whether it is liquid chromatography (LC) or gas chromatography (GC)?

  • Nature of the stationery phase
  • Elution process
  • Affinity of the analytes
  • Nature of the mobile phase (correct)

    • In chromatography, which phase carries the sample through a layer containing the stationery phase?

  • Mobile phase (correct)
  • Analytes
  • Stationery phase
  • Eluent

    • What is the term for the components of a sample mixture (solutes, analytes, or fractions) in chromatography?

    <p>Analytes</p> Signup and view all the answers

    What is the term for the received analyte after elution in chromatography?

    <p>Eluent or fraction</p> Signup and view all the answers

    Chromatography is a chemical method of separation.

    <p>False</p> Signup and view all the answers

    In column chromatography, the stationery phase is packed in a column.

    <p>True</p> Signup and view all the answers

    In gas chromatography (GC), the mobile phase is a liquid.

    <p>False</p> Signup and view all the answers

    In chromatography, the solutes distribute between the mobile and stationery phases based on their relative tendency to associate with each phase.

    <p>True</p> Signup and view all the answers

    Elution is the process of displacing weakly bound analytes from the stationery phase.

    <p>False</p> Signup and view all the answers

    Match the following terms with their meanings in chromatography:

    <p>Elution = Process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase Analytes = Components of a sample mixture separated by their differential distribution between two phases Mobile phase = Carries the sample through a layer containing the stationery phase during chromatography Stationary phase = Phase in chromatography that does not move during the process</p> Signup and view all the answers

    Match the following forms of chromatography with their descriptions:

    <p>Planar chromatography = Chromatographic method where the stationery phase is spread on a flat surface Column chromatography = Chromatographic method where the stationery phase is coated onto the inner surface or packed in a column Liquid chromatography (LC) = Column chromatography where the mobile phase is a liquid Gas chromatography (GC) = Column chromatography where the mobile phase is a gas</p> Signup and view all the answers

    Match the following statements with their correct descriptions:

    <p>Chromatography = Physical method of separation where components are separated by their differential distribution between two phases Affinity = Relative tendency of analytes to associate with either the mobile or stationery phase in chromatography TLC (Thin Layer Chromatography) = Planar chromatographic method where the stationery phase is spread as a thin layer on a flat surface HPLC (High-Performance Liquid Chromatography) = Type of liquid chromatography that uses high pressure to pass the mobile phase through the column</p> Signup and view all the answers

    Match the following terms with their correct definitions in chromatography:

    <p>Solutes = Components of a sample mixture that are separated during chromatography Distribution = Dependent on the relative tendency of analytes to associate with one of the two phases in chromatography Fraction = Received analyte after elution in chromatography HPTLC (High-Performance Thin Layer Chromatography) = Improved version of planar chromatographic method with better resolution and reproducibility</p> Signup and view all the answers

    Match the following descriptions with their correct forms of chromatography:

    <p>Packed column with coated inner surface = Column chromatography Mobile phase is a gas = Gas chromatography (GC) Spread on a flat surface as a thin layer = TLC (Thin Layer Chromatography) Uses high pressure for passing mobile phase through column = HPLC (High-Performance Liquid Chromatography)</p> Signup and view all the answers

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