15 Questions
What is the term for the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase?
Elution
In column chromatography, what determines whether it is liquid chromatography (LC) or gas chromatography (GC)?
Nature of the mobile phase
In chromatography, which phase carries the sample through a layer containing the stationery phase?
Mobile phase
What is the term for the components of a sample mixture (solutes, analytes, or fractions) in chromatography?
Analytes
What is the term for the received analyte after elution in chromatography?
Eluent or fraction
Chromatography is a chemical method of separation.
False
In column chromatography, the stationery phase is packed in a column.
True
In gas chromatography (GC), the mobile phase is a liquid.
False
In chromatography, the solutes distribute between the mobile and stationery phases based on their relative tendency to associate with each phase.
True
Elution is the process of displacing weakly bound analytes from the stationery phase.
False
Match the following terms with their meanings in chromatography:
Elution = Process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase Analytes = Components of a sample mixture separated by their differential distribution between two phases Mobile phase = Carries the sample through a layer containing the stationery phase during chromatography Stationary phase = Phase in chromatography that does not move during the process
Match the following forms of chromatography with their descriptions:
Planar chromatography = Chromatographic method where the stationery phase is spread on a flat surface Column chromatography = Chromatographic method where the stationery phase is coated onto the inner surface or packed in a column Liquid chromatography (LC) = Column chromatography where the mobile phase is a liquid Gas chromatography (GC) = Column chromatography where the mobile phase is a gas
Match the following statements with their correct descriptions:
Chromatography = Physical method of separation where components are separated by their differential distribution between two phases Affinity = Relative tendency of analytes to associate with either the mobile or stationery phase in chromatography TLC (Thin Layer Chromatography) = Planar chromatographic method where the stationery phase is spread as a thin layer on a flat surface HPLC (High-Performance Liquid Chromatography) = Type of liquid chromatography that uses high pressure to pass the mobile phase through the column
Match the following terms with their correct definitions in chromatography:
Solutes = Components of a sample mixture that are separated during chromatography Distribution = Dependent on the relative tendency of analytes to associate with one of the two phases in chromatography Fraction = Received analyte after elution in chromatography HPTLC (High-Performance Thin Layer Chromatography) = Improved version of planar chromatographic method with better resolution and reproducibility
Match the following descriptions with their correct forms of chromatography:
Packed column with coated inner surface = Column chromatography Mobile phase is a gas = Gas chromatography (GC) Spread on a flat surface as a thin layer = TLC (Thin Layer Chromatography) Uses high pressure for passing mobile phase through column = HPLC (High-Performance Liquid Chromatography)
Study Notes
Chromatography Basics
- Chromatography is a chemical method of separation.
Stationery Phase
- In column chromatography, the stationery phase is packed in a column.
Mobile Phase
- In chromatography, the mobile phase carries the sample through a layer containing the stationery phase.
Phases in Chromatography
- The mobile phase and stationery phase interact in chromatography, with solutes distributing between the two based on their relative tendency to associate with each phase.
Elution
- Elution is the process of displacing weakly bound analytes from the stationery phase.
Displacement
- Displacement is the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase.
Forms of Chromatography
- Liquid Chromatography (LC) and Gas Chromatography (GC) are determined by the state of the mobile phase.
- In GC, the mobile phase is a gas.
Sample Components
- The components of a sample mixture in chromatography are referred to as solutes, analytes, or fractions.
Received Analyte
- The received analyte after elution in chromatography is referred to as the eluate.
Test your knowledge of chromatography with this quiz focusing on the definition and basic concepts of this physical method of separation. From the components of a sample mixture to the differential distribution between two phases, this quiz covers fundamental chromatography principles.
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