Podcast
Questions and Answers
What is the term for the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase?
What is the term for the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase?
- Elution (correct)
- Eluent
- Chromatography
- Affinity
In column chromatography, what determines whether it is liquid chromatography (LC) or gas chromatography (GC)?
In column chromatography, what determines whether it is liquid chromatography (LC) or gas chromatography (GC)?
- Nature of the stationery phase
- Elution process
- Affinity of the analytes
- Nature of the mobile phase (correct)
In chromatography, which phase carries the sample through a layer containing the stationery phase?
In chromatography, which phase carries the sample through a layer containing the stationery phase?
- Mobile phase (correct)
- Analytes
- Stationery phase
- Eluent
What is the term for the components of a sample mixture (solutes, analytes, or fractions) in chromatography?
What is the term for the components of a sample mixture (solutes, analytes, or fractions) in chromatography?
What is the term for the received analyte after elution in chromatography?
What is the term for the received analyte after elution in chromatography?
Chromatography is a chemical method of separation.
Chromatography is a chemical method of separation.
In column chromatography, the stationery phase is packed in a column.
In column chromatography, the stationery phase is packed in a column.
In gas chromatography (GC), the mobile phase is a liquid.
In gas chromatography (GC), the mobile phase is a liquid.
In chromatography, the solutes distribute between the mobile and stationery phases based on their relative tendency to associate with each phase.
In chromatography, the solutes distribute between the mobile and stationery phases based on their relative tendency to associate with each phase.
Elution is the process of displacing weakly bound analytes from the stationery phase.
Elution is the process of displacing weakly bound analytes from the stationery phase.
Match the following terms with their meanings in chromatography:
Match the following terms with their meanings in chromatography:
Match the following forms of chromatography with their descriptions:
Match the following forms of chromatography with their descriptions:
Match the following statements with their correct descriptions:
Match the following statements with their correct descriptions:
Match the following terms with their correct definitions in chromatography:
Match the following terms with their correct definitions in chromatography:
Match the following descriptions with their correct forms of chromatography:
Match the following descriptions with their correct forms of chromatography:
Study Notes
Chromatography Basics
- Chromatography is a chemical method of separation.
Stationery Phase
- In column chromatography, the stationery phase is packed in a column.
Mobile Phase
- In chromatography, the mobile phase carries the sample through a layer containing the stationery phase.
Phases in Chromatography
- The mobile phase and stationery phase interact in chromatography, with solutes distributing between the two based on their relative tendency to associate with each phase.
Elution
- Elution is the process of displacing weakly bound analytes from the stationery phase.
Displacement
- Displacement is the process of displacing strongly bound analytes from the stationery phase by changing the physical or chemical nature of the mobile phase.
Forms of Chromatography
- Liquid Chromatography (LC) and Gas Chromatography (GC) are determined by the state of the mobile phase.
- In GC, the mobile phase is a gas.
Sample Components
- The components of a sample mixture in chromatography are referred to as solutes, analytes, or fractions.
Received Analyte
- The received analyte after elution in chromatography is referred to as the eluate.
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Description
Test your knowledge of chromatography with this quiz focusing on the definition and basic concepts of this physical method of separation. From the components of a sample mixture to the differential distribution between two phases, this quiz covers fundamental chromatography principles.