Chromatography Basics
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Questions and Answers

What is the primary purpose of chromatography?

  • To combine components into a mixture
  • To separate mixtures into their components (correct)
  • To create mixtures
  • To destroy unwanted components

    • What is chromatography used for?

  • To measure the temperature of a mixture
  • To change the color of a mixture
  • To produce chemical reactions
  • To analyze, identify, purify, and/or quantify the mixture or components (correct)

    • What is a result of using chromatography?

  • A mixture is left unchanged
  • A component is destroyed
  • A mixture is separated into its components (correct)
  • A mixture becomes more complex

    • What is the outcome of chromatography?

    <p>A mixture separated into its components</p> Signup and view all the answers

    What is the main objective of chromatography?

    <p>To separate and analyze mixtures</p> Signup and view all the answers

    What is the primary factor in separating components?

    <p>Solubility in the mobile phase</p> Signup and view all the answers

    What is the other factor that influences the separation of components?

    <p>Differential affinity to both phases</p> Signup and view all the answers

    What is the relationship between the mobile phase and the stationary phase?

    <p>The mobile phase and stationary phase have different properties</p> Signup and view all the answers

    Why is solubility in the mobile phase important for separation?

    <p>It affects the ability of components to separate</p> Signup and view all the answers

    What is the result of a component having a higher affinity to the stationary phase?

    <p>It moves slowly through the mobile phase</p> Signup and view all the answers

    What is the main objective of chromatography?

    <p>To separate and identify the components of a mixture</p> Signup and view all the answers

    What is the term for the process of separating and identifying the components of a mixture?

    <p>Chromatography</p> Signup and view all the answers

    What type of paper is used in chromatography?

    <p>Filter paper</p> Signup and view all the answers

    What is the purpose of the preparation step in chromatography?

    <p>To prepare the sample for analysis</p> Signup and view all the answers

    What is the term for the instruments used in chromatography?

    <p>Chromatographs</p> Signup and view all the answers

    What is the main purpose of chromatography?

    <p>To analyze a mixture and its components</p> Signup and view all the answers

    What is the term for determining the amount of a mixture and/or its components?

    <p>Quantify</p> Signup and view all the answers

    What is the process of separating components in order to isolate one of interest?

    <p>Purify</p> Signup and view all the answers

    Where is chromatography used to detect blood or alcohol levels?

    <p>Hospital</p> Signup and view all the answers

    What is the laboratory technique that separates components within a mixture?

    <p>Chromatography</p> Signup and view all the answers

    What is the purpose of chromatography in a manufacturing plant?

    <p>To purify a chemical needed to make a product</p> Signup and view all the answers

    What is the term for determining the identity of a mixture or its components?

    <p>Identify</p> Signup and view all the answers

    Where is chromatography used to compare a sample found at a crime scene to samples from suspects?

    <p>Law Enforcement Agency</p> Signup and view all the answers

    What is the purpose of chromatography in an environmental agency?

    <p>To quantify the amount of pollutants in the water supply</p> Signup and view all the answers

    What are the two media used in chromatography?

    <p>Mobile and stationary mediums</p> Signup and view all the answers

    What is the purpose of drawing a line 1 cm above the bottom edge of the filter paper strip?

    <p>To mark the starting point of the chromatogram</p> Signup and view all the answers

    How long should the strips be kept in the beakers for development?

    <p>Until the solution front is about 2 cm from the top of the strip</p> Signup and view all the answers

    What is the purpose of keeping the beakers covered?

    <p>To prevent contamination</p> Signup and view all the answers

    What is the next step after removing the strips from the beakers?

    <p>To let them dry</p> Signup and view all the answers

    How many strips of filter paper should be cut?

    <p>6</p> Signup and view all the answers

    What is the total duration of the experiment?

    <p>55 minutes</p> Signup and view all the answers

    Study Notes

    What is Chromatography?

    • Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.
    • It is used to examine a mixture, its components, and their relations to one another.

    Uses of Chromatography

    • Analyze: examine a mixture, its components, and their relations to one another.
    • Identify: determine the identity of a mixture or components based on known components.
    • Purify: separate components in order to isolate one of interest for further study.
    • Quantify: determine the amount of the mixture and/or the components present in the sample.

    Real-life Examples of Chromatography

    • Pharmaceutical Company: determine the amount of each chemical found in a new product.
    • Hospital: detect blood or alcohol levels in a patient's blood stream.
    • Law Enforcement: compare a sample found at a crime scene to samples from suspects.
    • Environmental Agency: determine the level of pollutants in the water supply.
    • Manufacturing Plant: purify a chemical needed to make a product.

    Definition of Chromatography

    • Chromatography is a laboratory technique that separates components within a mixture by using the differential affinities of the components for a mobile medium and for a stationary adsorbing medium through which they pass.
    • Separation of components depends on both their solubility in the mobile phase and their differential affinity to the mobile phase and the stationary phase.

    Chromatography Experiment

    • Prep time: 10 minutes.
    • Experiment time: 45 minutes.
    • Steps involved:
      • Cut 6 strips of filter paper.
      • Draw a line 1 cm above the bottom edge of the strip with a pencil.
      • Label each strip with its corresponding solution.
      • Place a spot from each pen on the starting line.
      • Place the strips in beakers.
      • Make sure the solution does not come above the start line.
      • Keep the beakers covered.
      • Let strips develop until the ascending solution front is about 2 cm from the top of the strip.
      • Remove the strips and let them dry.

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    Description

    Learn about chromatography, a technique used to separate and analyze mixtures into their components. Understand its applications and importance in various fields.

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