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Questions and Answers
What is the primary purpose of chromatography?
What is the primary purpose of chromatography?
- To combine components into a mixture
- To separate mixtures into their components (correct)
- To create mixtures
- To destroy unwanted components
What is chromatography used for?
What is chromatography used for?
- To measure the temperature of a mixture
- To change the color of a mixture
- To produce chemical reactions
- To analyze, identify, purify, and/or quantify the mixture or components (correct)
What is a result of using chromatography?
What is a result of using chromatography?
- A mixture is left unchanged
- A component is destroyed
- A mixture is separated into its components (correct)
- A mixture becomes more complex
What is the outcome of chromatography?
What is the outcome of chromatography?
What is the main objective of chromatography?
What is the main objective of chromatography?
What is the primary factor in separating components?
What is the primary factor in separating components?
What is the other factor that influences the separation of components?
What is the other factor that influences the separation of components?
What is the relationship between the mobile phase and the stationary phase?
What is the relationship between the mobile phase and the stationary phase?
Why is solubility in the mobile phase important for separation?
Why is solubility in the mobile phase important for separation?
What is the result of a component having a higher affinity to the stationary phase?
What is the result of a component having a higher affinity to the stationary phase?
What is the main objective of chromatography?
What is the main objective of chromatography?
What is the term for the process of separating and identifying the components of a mixture?
What is the term for the process of separating and identifying the components of a mixture?
What type of paper is used in chromatography?
What type of paper is used in chromatography?
What is the purpose of the preparation step in chromatography?
What is the purpose of the preparation step in chromatography?
What is the term for the instruments used in chromatography?
What is the term for the instruments used in chromatography?
What is the main purpose of chromatography?
What is the main purpose of chromatography?
What is the term for determining the amount of a mixture and/or its components?
What is the term for determining the amount of a mixture and/or its components?
What is the process of separating components in order to isolate one of interest?
What is the process of separating components in order to isolate one of interest?
Where is chromatography used to detect blood or alcohol levels?
Where is chromatography used to detect blood or alcohol levels?
What is the laboratory technique that separates components within a mixture?
What is the laboratory technique that separates components within a mixture?
What is the purpose of chromatography in a manufacturing plant?
What is the purpose of chromatography in a manufacturing plant?
What is the term for determining the identity of a mixture or its components?
What is the term for determining the identity of a mixture or its components?
Where is chromatography used to compare a sample found at a crime scene to samples from suspects?
Where is chromatography used to compare a sample found at a crime scene to samples from suspects?
What is the purpose of chromatography in an environmental agency?
What is the purpose of chromatography in an environmental agency?
What are the two media used in chromatography?
What are the two media used in chromatography?
What is the purpose of drawing a line 1 cm above the bottom edge of the filter paper strip?
What is the purpose of drawing a line 1 cm above the bottom edge of the filter paper strip?
How long should the strips be kept in the beakers for development?
How long should the strips be kept in the beakers for development?
What is the purpose of keeping the beakers covered?
What is the purpose of keeping the beakers covered?
What is the next step after removing the strips from the beakers?
What is the next step after removing the strips from the beakers?
How many strips of filter paper should be cut?
How many strips of filter paper should be cut?
What is the total duration of the experiment?
What is the total duration of the experiment?
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Study Notes
What is Chromatography?
- Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.
- It is used to examine a mixture, its components, and their relations to one another.
Uses of Chromatography
- Analyze: examine a mixture, its components, and their relations to one another.
- Identify: determine the identity of a mixture or components based on known components.
- Purify: separate components in order to isolate one of interest for further study.
- Quantify: determine the amount of the mixture and/or the components present in the sample.
Real-life Examples of Chromatography
- Pharmaceutical Company: determine the amount of each chemical found in a new product.
- Hospital: detect blood or alcohol levels in a patient's blood stream.
- Law Enforcement: compare a sample found at a crime scene to samples from suspects.
- Environmental Agency: determine the level of pollutants in the water supply.
- Manufacturing Plant: purify a chemical needed to make a product.
Definition of Chromatography
- Chromatography is a laboratory technique that separates components within a mixture by using the differential affinities of the components for a mobile medium and for a stationary adsorbing medium through which they pass.
- Separation of components depends on both their solubility in the mobile phase and their differential affinity to the mobile phase and the stationary phase.
Chromatography Experiment
- Prep time: 10 minutes.
- Experiment time: 45 minutes.
- Steps involved:
- Cut 6 strips of filter paper.
- Draw a line 1 cm above the bottom edge of the strip with a pencil.
- Label each strip with its corresponding solution.
- Place a spot from each pen on the starting line.
- Place the strips in beakers.
- Make sure the solution does not come above the start line.
- Keep the beakers covered.
- Let strips develop until the ascending solution front is about 2 cm from the top of the strip.
- Remove the strips and let them dry.
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