Chromatography and Caffeine Analysis Quiz

Chromatography Fundamentals

• The mobile phase in chromatography serves as a carrier for the sample, transporting it through the stationary phase to separate and identify the components.

Migration Rate of Compounds

• The migration rate of different compounds in chromatography is determined by their affinity for the stationary phase and the mobile phase, as well as their molecular size and shape.

Attractive Forces and Migration

• A substance with stronger attractive forces with the stationary phase migrates more slowly in chromatography because it interacts more strongly with the stationary phase, resulting in a slower migration rate.

Primary Purpose of Chromatography

• The primary purpose of chromatography is to separate, identify, and quantify the components of a mixture.

Role of the Stationary Phase

• The stationary phase in chromatography acts as a selective barrier, retaining certain components of the mixture based on their interactions with the stationary phase, allowing for separation and identification.