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Chromatographic Separation Resolution Quiz

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Chromatographic Separation Resolution Quiz

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Questions and Answers

What is the equation for efficiency (N) in terms of retention time (tR) and peak width (W)?

$N = 4 \left(\frac{tR}{W}\right)$

$N = 4 \left(\frac{tR,B}{W}\right)$

$N = 4 \left(\frac{tR,B}{W}\right)^2$

$N = 16 \left(\frac{tR}{W}\right)^2$ (correct)

What is the formula for retention factor (Rs) in terms of retention times (tR,A and tR,B), efficiency (N), and peak width (W)?

$Rs = \frac{tR,B - tR,A}{4(N)^{1/2}}$

$Rs = \frac{tR,B - tR,A}{4tR,B/(N)^{1/2}}$ (correct)

$Rs = \frac{tR,B - tR,A}{4}$

$Rs = \frac{tR,B - tR,A}{4tR,B}$

What is the relationship between Rs and N in equation (3)?

$Rs \propto N^{1/3}$

$Rs \propto N$

$Rs \propto (N)^{1/2}$ (correct)

$Rs \propto N^2$

What must be adjusted to obtain a good resolution according to the text?

<p>Efficiency, retention term, and selectivity</p> Signup and view all the answers

What does increasing the retention time lead to according to the text?

<p>Band broadening</p> Signup and view all the answers

Study Notes

Resolution in Chromatography

Resolution (Rs) is a fundamental characteristic of chromatographic separation, measuring how well two peaks are separated from each other.
A higher resolution indicates better separation of peaks, which is essential for effective separation.

Factors Affecting Resolution

The greater the separation of zones (∆Z) from each other, the larger the resolution (Rs).
The smaller the zone width (WA or WB), the larger the resolution (Rs).

Calculating Resolution

Resolution (Rs) can be calculated using the chromatogram curve: Rs = ∆Z / (WA+WB)/2 = 2 ∆Z / (WA+WB)
If the zone widths are equal (WA=WB=W), the formula simplifies to: Rs = (tR,B – tR,A) /W

Key Equation

Rs = 2 ∆Z / (WA+WB)
Rs = (tR,B – tR,A) /W (when WA=WB=W)

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Description

Test your knowledge on chromatographic separation resolution, which is a fundamental aspect of chromatography. Learn about measuring the separation of peaks and understanding the concept of resolution in chromatography.