5 Questions
What is the equation for efficiency (N) in terms of retention time (tR) and peak width (W)?
$N = 16 \left(\frac{tR}{W}\right)^2$
What is the formula for retention factor (Rs) in terms of retention times (tR,A and tR,B), efficiency (N), and peak width (W)?
$Rs = \frac{tR,B - tR,A}{4tR,B/(N)^{1/2}}$
What is the relationship between Rs and N in equation (3)?
$Rs \propto (N)^{1/2}$
What must be adjusted to obtain a good resolution according to the text?
Efficiency, retention term, and selectivity
What does increasing the retention time lead to according to the text?
Band broadening
Study Notes
Resolution in Chromatography
- Resolution (Rs) is a fundamental characteristic of chromatographic separation, measuring how well two peaks are separated from each other.
- A higher resolution indicates better separation of peaks, which is essential for effective separation.
Factors Affecting Resolution
- The greater the separation of zones (∆Z) from each other, the larger the resolution (Rs).
- The smaller the zone width (WA or WB), the larger the resolution (Rs).
Calculating Resolution
- Resolution (Rs) can be calculated using the chromatogram curve: Rs = ∆Z / (WA+WB)/2 = 2 ∆Z / (WA+WB)
- If the zone widths are equal (WA=WB=W), the formula simplifies to: Rs = (tR,B – tR,A) /W
Key Equation
- Rs = 2 ∆Z / (WA+WB)
- Rs = (tR,B – tR,A) /W (when WA=WB=W)
Test your knowledge on chromatographic separation resolution, which is a fundamental aspect of chromatography. Learn about measuring the separation of peaks and understanding the concept of resolution in chromatography.
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