12 Questions
What is the primary purpose of using thin-layer chromatography?
Determining the number of components in a sample
Which type of molecule would move slowly in thin-layer chromatography?
A molecule strongly adsorbed on the stationary phase
Why are alumina and silica gel commonly used as the stationary phase in TLC?
To improve the resolution of the separated components
How does thin-layer chromatography differ from column chromatography in terms of time requirement?
TLC is quicker, requiring only 2-5 minutes
What is the advantage of using TLC when compared to other chromatographic techniques?
Needs very small quantities of material (2-20µg)
In TLC, what kind of molecules would elute most quickly through the plate?
Molecules with low solubility in eluent
What is the main principle behind chromatography techniques?
Differential solubility of mixture components in the two phases.
In thin-layer chromatography (TLC), what is the function of the stationary phase?
To bind the sample to the solid phase.
Which type of chromatography uses a thin layer of stationary phase over a flat surface?
Thin-layer chromatography
What forces are acting on a sample in TLC when subjected to two opposing forces?
Solubility of the sample and adsorption forces binding it to the solid phase.
Which chromatographic method relies on polarity differences to separate materials?
Thin-layer chromatography
What is the most critical factor for effective separation in chromatographic methods?
Differential solubility of mixture components.
Explore how chromatography revolutionized experimental organic chemistry by separating mixtures and isolating pure substances with the use of stationary and mobile phases. Learn about column, thin-layer, and paper chromatography methods.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
