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Chromatographic Methods in Organic Chemistry

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Chromatographic Methods in Organic Chemistry

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Questions and Answers

What is the primary purpose of using thin-layer chromatography?

Following the progress of a reaction in real-time

Detecting certain substances in a pure mixture

Determining the number of components in a sample (correct)

Identifying the mobile phase in chromatography

Which type of molecule would move slowly in thin-layer chromatography?

A molecule with high solubility in the eluent

A molecule dissolved readily in the liquid phase

A molecule strongly adsorbed on the stationary phase (correct)

A molecule that does not interact with the plate

Why are alumina and silica gel commonly used as the stationary phase in TLC?

To enhance the solubility of the components

To increase the speed of the separation process

To improve the resolution of the separated components (correct)

To decrease the interaction between molecules

How does thin-layer chromatography differ from column chromatography in terms of time requirement?

TLC is quicker, requiring only 2-5 minutes Signup and view all the answers

What is the advantage of using TLC when compared to other chromatographic techniques?

Needs very small quantities of material (2-20µg) Signup and view all the answers

In TLC, what kind of molecules would elute most quickly through the plate?

Molecules with low solubility in eluent Signup and view all the answers

What is the main principle behind chromatography techniques?

Differential solubility of mixture components in the two phases. Signup and view all the answers

In thin-layer chromatography (TLC), what is the function of the stationary phase?

To bind the sample to the solid phase. Signup and view all the answers

Which type of chromatography uses a thin layer of stationary phase over a flat surface?

Thin-layer chromatography Signup and view all the answers

What forces are acting on a sample in TLC when subjected to two opposing forces?

Solubility of the sample and adsorption forces binding it to the solid phase. Signup and view all the answers

Which chromatographic method relies on polarity differences to separate materials?

Thin-layer chromatography Signup and view all the answers

What is the most critical factor for effective separation in chromatographic methods?

Differential solubility of mixture components. Signup and view all the answers