Podcast
Questions and Answers
What is the primary purpose of using thin-layer chromatography?
What is the primary purpose of using thin-layer chromatography?
- Following the progress of a reaction in real-time
- Detecting certain substances in a pure mixture
- Determining the number of components in a sample (correct)
- Identifying the mobile phase in chromatography
Which type of molecule would move slowly in thin-layer chromatography?
Which type of molecule would move slowly in thin-layer chromatography?
- A molecule with high solubility in the eluent
- A molecule dissolved readily in the liquid phase
- A molecule strongly adsorbed on the stationary phase (correct)
- A molecule that does not interact with the plate
Why are alumina and silica gel commonly used as the stationary phase in TLC?
Why are alumina and silica gel commonly used as the stationary phase in TLC?
- To enhance the solubility of the components
- To increase the speed of the separation process
- To improve the resolution of the separated components (correct)
- To decrease the interaction between molecules
How does thin-layer chromatography differ from column chromatography in terms of time requirement?
How does thin-layer chromatography differ from column chromatography in terms of time requirement?
What is the advantage of using TLC when compared to other chromatographic techniques?
What is the advantage of using TLC when compared to other chromatographic techniques?
In TLC, what kind of molecules would elute most quickly through the plate?
In TLC, what kind of molecules would elute most quickly through the plate?
What is the main principle behind chromatography techniques?
What is the main principle behind chromatography techniques?
In thin-layer chromatography (TLC), what is the function of the stationary phase?
In thin-layer chromatography (TLC), what is the function of the stationary phase?
Which type of chromatography uses a thin layer of stationary phase over a flat surface?
Which type of chromatography uses a thin layer of stationary phase over a flat surface?
What forces are acting on a sample in TLC when subjected to two opposing forces?
What forces are acting on a sample in TLC when subjected to two opposing forces?
Which chromatographic method relies on polarity differences to separate materials?
Which chromatographic method relies on polarity differences to separate materials?
What is the most critical factor for effective separation in chromatographic methods?
What is the most critical factor for effective separation in chromatographic methods?
