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Chloroplast Structure
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Chloroplast Structure

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Questions and Answers

What are the three distinct membranes found in the chloroplast?

The outer membrane, the inner membrane, and the thylakoid membrane

What is the function of the thylakoid membrane in the chloroplast?

It contains all the energy-generating systems of the chloroplast, including the green pigment chlorophyll

What is the name of the flattened disc-like sacs formed by the thylakoid membrane?

Thylakoids

What are the three separate internal compartments of the chloroplast?

<p>The intermembrane space, the stroma, and the thylakoid space</p> Signup and view all the answers

What is the role of the stroma in the chloroplast?

<p>It contains photosynthetic metabolic enzymes, catalyse CO2 fixation, ribosomes, DNA, and RNA</p> Signup and view all the answers

What is the mechanism by which chloroplasts and mitochondria generate ATP?

<p>Chemiosmosis/chemiosmotic coupling</p> Signup and view all the answers

What is the energy source and reducing power used to reduce CO2 in the light-independent reactions of photosynthesis?

<p>ATP and NADPH produced by the light reactions</p> Signup and view all the answers

What is the purpose of the proton pump in the first stage of chemiosmosis?

<p>To pump protons (H+) across a membrane, creating a proton gradient</p> Signup and view all the answers

What is the source of the protons used to create the proton gradient in chemiosmosis?

<p>Water</p> Signup and view all the answers

What is the role of ATP synthase in chemiosmosis?

<p>To generate ATP as H+ diffuses back across the membrane</p> Signup and view all the answers

What is the relationship between the proton gradient and ATP synthesis?

<p>The energy stored in the proton gradient is used to drive ATP synthesis</p> Signup and view all the answers

In which organelles does chemiosmosis occur?

<p>Mitochondria and chloroplasts</p> Signup and view all the answers

Study Notes

Chloroplast Structure

  • Chloroplasts have three distinct membranes: outer, inner, and thylakoid membranes.
  • The thylakoid membrane forms flattened disc-like sacs called thylakoids, which contain energy-generating systems, including chlorophyll.
  • Individual thylakoids are interconnected via lamellae and tend to stack to form grana.

Chloroplast Compartments

  • Chloroplasts have three internal compartments: intermembrane space, stroma, and thylakoid space.
  • The stroma contains photosynthetic metabolic enzymes, ribosomes, DNA, and RNA.

Photosynthesis

  • Both stages of photosynthesis depend on the chloroplast.
  • Stage 1: Photolysis of H2O produces O2, ATP, and NADPH.
  • Stage 2: ATP and NADPH are used to reduce CO2, producing sugar.

Light-Capturing Systems

  • Light-absorbing chlorophyll, photosystem protein complexes, electron-transport chains, and ATP synthase are located on the thylakoid membrane.
  • Water is split by photosystem II on the side of the membrane facing the thylakoid space.

ATP Synthesis

  • The diffusion of H+ from the thylakoid space back to the stroma powers ATP synthase.
  • ATP and NADPH are produced on the side facing the stroma, where the Calvin cycle takes place.
  • Chloroplasts and mitochondria generate ATP by the same mechanism: chemiosmosis/chemiosmotic coupling.

Chemiosmosis

  • Chemiosmosis is a mechanism that uses energy stored in a transmembrane proton gradient to drive an energy-requiring process, such as ATP synthesis.
  • An electron transport chain pumps protons across a membrane as electrons are passed along a series of more electronegative carriers, creating a proton gradient.
  • ATP synthase harnesses the proton-motive force to generate ATP as H+ diffuses back across the membrane.

Comparison of Chemiosmosis

  • Chemiosmotic coupling is used in both mitochondria and chloroplasts to synthesize ATP.
  • The proton gradient produced in one phase serves as an energy store that can be used to drive various energy-requiring reactions.

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Test your knowledge of the basic structure of chloroplasts, including the different membranes and internal compartments. Learn about thylakoids, grana, and lamellae. Identify the components that make up this vital plant cell organelle.

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