Chemical Tests in Biochemistry
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Questions and Answers

What is the specific purpose of the Sakaguchi test?

  • Detecting the presence of cysteine
  • Identifying the presence of amines
  • Checking for the presence of proteins
  • Detecting the presence of sulfur-containing amino acids (correct)

Which components are used in the Sakaguchi test?

  • 1% NaOH and a solution of cuprous sulfate
  • NaOH, α-naphthol, and Bromine solution (correct)
  • Sulfuric acid and cuprous sulfate
  • NaOH, Glyoxylic acid, and Bromine solution

What color indicates a positive result in the Sakaguchi test?

  • Red-colored complex (correct)
  • Yellow/red-orange
  • Black precipitate
  • Purple

Which amino acid is specifically targeted by the Sakaguchi test?

<p>Arginine (C)</p> Signup and view all the answers

Which test is used for detecting Indole group presence?

<p>Hopkin's Cole Test (C)</p> Signup and view all the answers

What is the color of a positive result in the Hopkin's Cole Test?

<p>Purple Color on the surface (C)</p> Signup and view all the answers

What is the purpose of the Sakaguchi Test?

<p>To determine the presence of ketone bodies (B)</p> Signup and view all the answers

Which reagent is used in the Sakaguchi Test?

<p>Strong ammonia (D)</p> Signup and view all the answers

What chemical reaction is observed in the Sakaguchi Test?

<p>Creation of a black cloud with Lugol’s solution (B)</p> Signup and view all the answers

Which amino acids are associated with the test for creatinine?

<p>Glycine, Arginine, Methionine (GAM) (C)</p> Signup and view all the answers

Which condition is characterized by blood glucose greater than 180 mg/dL and blood in the urine?

<p>Hyperglycemia (D)</p> Signup and view all the answers

What is the suspected cause of type 1 diabetes according to the text?

<p>An autoimmune reaction (A)</p> Signup and view all the answers

What is the purpose of the Sakaguchi test?

<p>To detect the presence of bile pigments in urine (C)</p> Signup and view all the answers

What is formed when 5 ml of urine is overlaid with tincture of alcoholic iodine in the Sakaguchi test?

<p>Emerald at the point of contact (D)</p> Signup and view all the answers

Which type of bile pigment causes yellow discoloration of skin in jaundice patients?

<p>Bilirubin (D)</p> Signup and view all the answers

What happens when hydrogen peroxide is added to Guaiac solution in the test for occult blood?

<p>Formation of a blue color (A)</p> Signup and view all the answers

What does the Sakaguchi test primarily detect in urine?

<p>Presence of bile pigments (A)</p> Signup and view all the answers

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