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Chemical Tests in Biochemistry

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Questions and Answers

What is the specific purpose of the Sakaguchi test?

Detecting the presence of cysteine

Identifying the presence of amines

Checking for the presence of proteins

Detecting the presence of sulfur-containing amino acids (correct)

Which components are used in the Sakaguchi test?

1% NaOH and a solution of cuprous sulfate

NaOH, α-naphthol, and Bromine solution (correct)

Sulfuric acid and cuprous sulfate

NaOH, Glyoxylic acid, and Bromine solution

What color indicates a positive result in the Sakaguchi test?

Red-colored complex (correct)

Yellow/red-orange

Black precipitate

Purple

Which amino acid is specifically targeted by the Sakaguchi test?

Arginine Signup and view all the answers

Which test is used for detecting Indole group presence?

Hopkin's Cole Test Signup and view all the answers

What is the color of a positive result in the Hopkin's Cole Test?

Purple Color on the surface Signup and view all the answers

What is the purpose of the Sakaguchi Test?

To determine the presence of ketone bodies Signup and view all the answers

Which reagent is used in the Sakaguchi Test?

Strong ammonia Signup and view all the answers

What chemical reaction is observed in the Sakaguchi Test?

Creation of a black cloud with Lugol’s solution Signup and view all the answers

Which amino acids are associated with the test for creatinine?

Glycine, Arginine, Methionine (GAM) Signup and view all the answers

Which condition is characterized by blood glucose greater than 180 mg/dL and blood in the urine?

Hyperglycemia Signup and view all the answers

What is the suspected cause of type 1 diabetes according to the text?

An autoimmune reaction Signup and view all the answers

What is the purpose of the Sakaguchi test?

To detect the presence of bile pigments in urine Signup and view all the answers

What is formed when 5 ml of urine is overlaid with tincture of alcoholic iodine in the Sakaguchi test?

Emerald at the point of contact Signup and view all the answers

Which type of bile pigment causes yellow discoloration of skin in jaundice patients?

Bilirubin Signup and view all the answers

What happens when hydrogen peroxide is added to Guaiac solution in the test for occult blood?

Formation of a blue color Signup and view all the answers

What does the Sakaguchi test primarily detect in urine?

Presence of bile pigments Signup and view all the answers