Chemical Sterilization Agents Quiz

To compare the effectiveness of the new chemical agent to the reference standard (phenol)

They disorganize the cell membrane lipid structure.

Sterilization destroys all forms of microbial life including spores, while disinfection only destroys microbes that cause disease and may not be effective in killing spores.

<p>Antisepsis is the destruction or inhibition of microorganisms in living tissue to prevent harmful infections. Disinfection, on the other hand, targets microbes that cause disease but may not be effective in killing spores.</p> Signup and view all the answers

<p>They cause conformational alteration of proteins, resulting in irregular looping and coiling of the polypeptide chain.</p> Signup and view all the answers

<p>Denatures protein</p> Signup and view all the answers

<p>Aseptic techniques are used in microbiological research, food preservation, and disease prevention. For example, maintaining sterility during surgical procedures.</p> Signup and view all the answers

<p>They interact with functional groups of proteins and nucleic acids.</p> Signup and view all the answers

<p>The concentration of the agent, time of exposure, and temperature can influence the efficacy of chemical agents.</p> Signup and view all the answers

<p>Due to their neurotoxic effects.</p> Signup and view all the answers

<p>More rapid killing</p> Signup and view all the answers

<p>Using sterile apparatus and culture media is crucial to prevent contamination and ensure the purity of the cultures being studied.</p> Signup and view all the answers

<p>70%</p> Signup and view all the answers

<p>160 - 1000C for 1 hour</p> Signup and view all the answers

<p>Intermittent sterilization</p> Signup and view all the answers

<p>Chemical agents used for sterilization and disinfection do not show selectivity and can destroy any type of microbe, unlike antibiotics.</p> Signup and view all the answers

<p>62oC for 30 minutes (Holder method) or 72oC for 15 seconds (Flash method)</p> Signup and view all the answers

<p>To discourage bacterial growth</p> Signup and view all the answers

<p>Sterilize medical-surgical devices that would be damaged by heat.</p> Signup and view all the answers

<p>Never be toxic to cells, never be corrosive, should never change nature of skin.</p> Signup and view all the answers

<p>To compare the potency of a disinfectant with that of phenol.</p> Signup and view all the answers

<p>The lowest concentration of disinfectant that inhibits the growth of S.typhi in a nutrient medium.</p> Signup and view all the answers

<p>To measure the capacity of the disinfectant to cope with successive bacterial invasions.</p> Signup and view all the answers

<p>Heat</p> Signup and view all the answers

<p>When water is boiled at increased pressure, hot saturated steam is formed which penetrates and gives up its latent heat when it condenses on cooler objects.</p> Signup and view all the answers

<p>High temperature, high latent heat, ability to form water of condensation, and contraction in volume during condensation.</p> Signup and view all the answers

<p>Pressure of 15 lb/in², temperature of 121°C, for 15 minutes.</p> Signup and view all the answers

<p>Color change of autoclave tape from blue to brown-black.</p> Signup and view all the answers

<p>Using paper strips impregnated with spores of Bacillus subtilis subsp. niger.</p> Signup and view all the answers

<p>Recording temperature and time of each cycle, using heat-sensitive autoclave tape, and using biological indicators.</p> Signup and view all the answers