Chapter 13: Diagnosing Infectious Diseases
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Questions and Answers

What is the maximum recommended time for a urine specimen to be stored in a refrigerator before being cultured?

  • 12 hours
  • 48 hours
  • 24 hours (correct)
  • 72 hours
  • What is the primary reason for collecting blood cultures in pairs, one aerobic and one anaerobic?

  • To test for the presence of both aerobic and anaerobic bacteria. (correct)
  • To ensure the sample is large enough for analysis.
  • To minimize the risk of contamination during sample collection.
  • To increase the likelihood of finding bacteria in the blood.
  • Which of these is NOT a precaution for handling specimens?

  • Protect specimens from direct sunlight.
  • Transport the specimen at room temperature. (correct)
  • Use sterile equipment to collect the sample.
  • Handle the specimen as quickly as possible.
  • Which of the following conditions refers to the presence of bacteria in the bloodstream?

    <p>Bacteremia (D)</p> Signup and view all the answers

    What is the primary potential consequence of failing to refrigerate a urine specimen within 30 minutes of collection?

    <p>Bacterial overgrowth and inaccurate results. (B)</p> Signup and view all the answers

    What is the purpose of performing a colony count in urine culture analysis?

    <p>To determine the number of viable bacteria in the urine. (B)</p> Signup and view all the answers

    Which of the following is NOT essential in a completed lab requisition?

    <p>The type of bacteria suspected. (D)</p> Signup and view all the answers

    What is the primary reason that improper collection techniques can negatively impact the results of a culture?

    <p>They can introduce foreign substances into the specimen. (E)</p> Signup and view all the answers

    Why is clean-catch, midstream urine (CCMS) collection preferred for diagnosing urinary tract infections?

    <p>It minimizes contamination from the external urethra. (B)</p> Signup and view all the answers

    What is the significance of a colony count of 100,000 CFU/mL or higher in a urine culture?

    <p>It could indicate potential contamination during specimen collection or handling. (C)</p> Signup and view all the answers

    Which of the following is NOT a method used for collecting urine specimens for UTI diagnosis?

    <p>Blood culture (A)</p> Signup and view all the answers

    What is the primary purpose of antimicrobial susceptibility testing in diagnosing a UTI?

    <p>To determine the effectiveness of different antibiotics against the isolated bacteria. (B)</p> Signup and view all the answers

    What is the significance of finding two or more bacteria per 1,000 microscopic fields in a Gram-stained urine smear?

    <p>It strongly supports a diagnosis of UTI, even with a low CFU count. (D)</p> Signup and view all the answers

    Why is it important to cleanse the urethral opening before collecting a clean-catch midstream urine sample?

    <p>To reduce the risk of contamination from the surrounding skin and urethra. (B)</p> Signup and view all the answers

    Which of the following is a potential reason for a high colony count in a urine culture even if the patient does not have a UTI?

    <p>The urine sample was improperly collected or handled. (B)</p> Signup and view all the answers

    Why is suprapubic needle aspiration considered more invasive for collecting urine samples?

    <p>It requires the use of a needle to directly access the bladder. (A)</p> Signup and view all the answers

    Which of the following is an example of a semi-critical item?

    <p>Endoscope (B)</p> Signup and view all the answers

    What is the main difference between medical asepsis and surgical asepsis?

    <p>Medical asepsis focuses on reducing pathogens, while surgical asepsis aims to eliminate all microbes. (A)</p> Signup and view all the answers

    What is the primary purpose of Standard Precautions?

    <p>To prevent the spread of infection from patient to patient and healthcare worker to patient. (B)</p> Signup and view all the answers

    Which of the following is NOT a requirement for surgical asepsis?

    <p>Properly cleaning and disinfecting the operating room. (A)</p> Signup and view all the answers

    Which of these is the most effective method for preventing the spread of infections?

    <p>Hand hygiene. (D)</p> Signup and view all the answers

    According to the OSHA Bloodborne Pathogen Standard, which of the following topics must be included in a written Exposure Control Plan?

    <p>Postexposure follow-up procedures (B)</p> Signup and view all the answers

    Which of these is NOT a bloodborne illness covered by the OSHA Bloodborne Pathogen Standard?

    <p>Tuberculosis (B)</p> Signup and view all the answers

    Which of the following procedures requires the use of surgical asepsis?

    <p>Inserting a urinary catheter (D)</p> Signup and view all the answers

    What is the primary method of infection prevention emphasized?

    <p>Wearing sterile masks and gloves (C)</p> Signup and view all the answers

    What should be done before and after patient contact to prevent infection?

    <p>Wash hands thoroughly (A)</p> Signup and view all the answers

    In which scenario are alcohol-based hand rubs recommended?

    <p>For unsoiled hands (A)</p> Signup and view all the answers

    What type of rooms are used for immunocompromised patients to prevent airborne infections?

    <p>Positive pressure rooms (B)</p> Signup and view all the answers

    What is NOT recommended for healthcare personnel during patient care?

    <p>Wearing artificial nails (C)</p> Signup and view all the answers

    Which of the following is NOT a general precaution for specimen collection and transport?

    <p>Transport specimens to the lab using any available method for fastest delivery. (B)</p> Signup and view all the answers

    When is it ideal to collect a specimen for an infectious disease diagnosis, if possible?

    <p>At the onset of symptoms. (A)</p> Signup and view all the answers

    What is the PRIMARY role of healthcare professionals in specimen collection and transport?

    <p>Selecting, collecting, and transporting specimens, and communicating findings to clinicians. (B)</p> Signup and view all the answers

    Which of the following statements accurately reflects the importance of specimen quality in infectious disease diagnosis?

    <p>Poor specimen quality can lead to inaccurate and unreliable laboratory results. (B)</p> Signup and view all the answers

    Why is it important to collect specimens aseptically?

    <p>To ensure the accurate identification of the pathogen without confounding results due to extraneous microbes. (D)</p> Signup and view all the answers

    What is the role of laboratory procedure manuals (P&P manuals) in specimen handling?

    <p>Providing detailed guidelines on the collection, transport, and processing of clinical specimens. (C)</p> Signup and view all the answers

    Which of the following is NOT a factor that influences the selection of a clinical specimen type to diagnose an infection?

    <p>The patient's height and weight. (D)</p> Signup and view all the answers

    How can a healthcare professional ensure proper communication with a microbiology lab about a specimen?

    <p>By using a standardized specimen requisition form with detailed patient information and specimen details. (B)</p> Signup and view all the answers

    What is the rate of Central Line-Associated Bloodstream Infections (CLABSI) in pediatric ICUs?

    <p>8.23 per 1,000 catheter-days (B)</p> Signup and view all the answers

    Which of the following is NOT a category of Healthcare-Associated Infections (HAIs)?

    <p>Community-Acquired Infections (CAIs) (A)</p> Signup and view all the answers

    What is the main reason for the importance of standardized procedures in healthcare settings?

    <p>To prevent the spread of infectious diseases. (B)</p> Signup and view all the answers

    What is the predominant pathogen associated with Healthcare-Associated Infections (HAIs)?

    <p>Gram-Positive Cocci (D)</p> Signup and view all the answers

    Which of the following is a key element in effectively implementing procedures to prevent HAIs?

    <p>Having sufficient resources. (A)</p> Signup and view all the answers

    What is the reported rate of Catheter-Associated Urinary Tract Infections (CAUTI) in pediatric ICUs?

    <p>No reported cases (A)</p> Signup and view all the answers

    Which of the following is NOT considered a healthcare setting?

    <p>Private Residence (A)</p> Signup and view all the answers

    Flashcards

    Clinical Specimens

    Samples like blood or urine collected for infection diagnosis.

    Specimen Quality

    Refers to the integrity and correctness of collected specimens.

    Specimen Selection

    Choosing the correct type of specimen for testing.

    Aseptic Collection

    Gathering specimens without contamination.

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    Transport Procedures

    Guidelines for safely moving specimens to labs.

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    Collaboration with Microbiology Lab

    Healthcare professionals working with labs for accurate diagnosis.

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    Timing of Collection

    Collecting specimens when the disease is acute.

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    Sterile Containers

    Clean containers used to prevent specimen contamination.

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    Specimen Handling Precautions

    Precautions to follow during specimen collection and transport to ensure accuracy.

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    Transport Specimens Promptly

    Specimens must be taken to the lab quickly after collection to avoid degradation.

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    Protect from Heat, Cold, Oxygen

    Specimens must be shielded from extreme temperatures and oxygen for certain pathogens.

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    Label Specimens Correctly

    Correct labeling of specimens is necessary for identification and processing in the lab.

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    Prevent Contamination

    Handling specimens with care to avoid introducing foreign microorganisms.

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    Urine Specimen Processing Time

    Urine samples should be processed within 30 minutes or refrigerated at 4°C.

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    Bacteremia

    The presence of bacteria in the bloodstream.

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    Urine Culture Analysis

    A method to estimate the quantity of viable bacteria in urine via colony count.

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    Aseptic Technique

    Methods to prevent contamination during medical procedures.

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    Colony-Forming Units (CFU)

    A measurement used to estimate the number of viable bacteria in a sample.

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    Clean-Catch, Midstream Urine (CCMS)

    A method to collect urine minimizing contamination.

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    Indication of Urinary Tract Infection (UTI)

    A colony count of 100,000 CFU/mL or higher typically indicates a UTI.

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    Specimen Contamination

    Unwanted microorganisms in a urine sample that can affect results.

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    Antimicrobial Susceptibility Testing

    A test to evaluate the effectiveness of antibiotics on bacteria.

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    Gram-Stained Urine Smear

    A diagnostic test to observe bacteria in urine under microscopy.

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    Microscopic Field Count

    Counting bacteria in specific areas under a microscope to assess infection.

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    Semi-critical Items

    Items that contact mucous membranes or non-intact skin, needing high-level disinfection.

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    Noncritical Items

    Items that only contact intact skin; require low-level disinfection.

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    Medical Asepsis

    Clean technique that excludes pathogens; aims for cleanliness.

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    Surgical Asepsis

    Sterile technique that excludes all microbes; ensures items are sterile.

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    Universal Precautions

    Infection control measures applied to all patients, regardless of infection status.

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    Bloodborne Pathogens

    Microorganisms in blood that can cause disease, e.g., HIV, HBV, HCV.

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    Standard Precautions

    Combines Universal Precautions and Body Substance Isolation precautions.

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    Hand Hygiene

    Practices to maintain hand cleanliness and prevent infections.

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    Device-Associated Infections

    Infections acquired in healthcare settings due to medical devices.

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    Ventilator-Associated Pneumonia (VAP)

    A type of pneumonia acquired by patients using a ventilator.

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    Central Line-Associated Bloodstream Infections (CLABSI)

    Infections that occur when bacteria enter the bloodstream through a central line.

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    Catheter-Associated Urinary Tract Infections (CAUTI)

    Infections caused by catheter use that affect the urinary tract.

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    Healthcare-Associated Infections (HAIs)

    Infections that develop within healthcare settings after admission.

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    Gram-Positive Cocci

    A group of bacteria that includes pathogens like Staphylococcus aureus.

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    Contact Transmission

    Bacterial spread through direct person-to-person contact.

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    Preventable Infections

    Infections that can be avoided with proper procedures.

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    Infection Prevention Method

    Wearing sterile PPE like masks, gloves, and gowns to prevent infections.

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    Antimicrobial Agents

    Substances used in specific scenarios to kill or inhibit microbes.

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    Airborne Infection Isolation Rooms (AIIRs)

    Rooms with negative pressure and HEPA filtration to contain airborne pathogens.

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    Recommended Vaccines

    Vaccines suggested for healthcare personnel, including Hepatitis B and Influenza.

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    Study Notes

    Chapter 13: Diagnosing Infectious Diseases

    • Accurate diagnosis of infectious diseases relies on patient history, physical examination, symptom evaluation, and proper specimen handling.
    • Clinical specimens, such as blood, urine, and feces, are collected to diagnose or monitor infections.
    • High-quality specimens are crucial for accurate lab results.
    • Healthcare professionals play a vital role in specimen collection and transport.

    Importance of High-Quality Specimens

    • Specimen quality directly impacts the accuracy of lab results.

    • Proper specimen selection, collection, and transport are required for high-quality specimens.

    • Lab procedures and guidelines are essential to ensure correct handling of specimens.

    • Proper Selection, Collection, and Transport

    • Select the correct specimen type.

    • Collect aseptically to minimize contamination.

    • Collect from the site most likely to contain the pathogen.

    • Collect before antimicrobial therapy begins, if possible.

    • Collect during the acute stage of the disease.

    • Collect sufficient specimen quantity.

    • Use sterile containers for transport and storage

    • Protect specimens from heat, cold, and oxygen, if needed.

    • Label specimens correctly and include a completed lab requisition.

    Role of Healthcare Professionals

    • Close collaboration between clinicians and the microbiology lab is essential.
    • Healthcare professionals select, collect, and transport specimens, and communicate findings to clinicians.
    • Safety precautions (Standard Precautions) must be strictly followed during specimen handling.

    Types of Clinical Specimens

    • Blood: Sterile within the body; bacteremia (bacteria in the bloodstream), septicemia (serious disease with bacteria/toxins in bloodstream). Blood cultures are typically collected in pairs. Aseptic technique is crucial
    • Urine: Normally sterile within the bladder, contamination can occur during urination due to contact with the distal urethra.
    • Sputum: Collected from patients with respiratory infections (pneumonia, tuberculosis).
    • CSF (Cerebrospinal Fluid): Obtained via lumbar puncture, crucial for diagnosing meningitis, encephalitis, and meningoencephalitis. Treated as a STAT specimen. Do not refrigerate.
    • Fecal Specimens: Collected and processed promptly to prevent pH changes.
    • Throat Swabs: Primarily for diagnosing strep throat (Streptococcus pyogenes).
    • Wound Specimens: Aspirates are preferred over swabs for minimizing contamination.

    Clinical Microbiology Laboratory (CML)

    • Responsible for diagnosing infectious diseases.
    • Responsibilities: Process samples, isolate pathogens, identify pathogens, antimicrobial susceptibility testing.
    • Sections: Bacteriology, Mycology, Parasitology, Virology, Mycobacteriology.

    Virology

    • Diagnoses viral infections, uses techniques like immunodiagnostic tests, electron microscopy, cell culture, and molecular methods.

    Mycobacteriology

    • Focuses on tuberculosis diagnosis, uses acid-fast staining, culture, and biochemical tests.

    Pathology Department

    • Divided into Anatomical (autopsies, tissue exam, cytology) and Clinical Pathology (CML, Clinical Chemistry, Hematology, Blood Bank, Immunology).

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    Description

    This quiz covers the essential elements of diagnosing infectious diseases, focusing on the importance of high-quality clinical specimens. Learn about the procedures for specimen selection, collection, and transport that are crucial for accurate lab results. Understand the role of healthcare professionals in the diagnostic process.

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