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Questions and Answers
What is the maximum recommended time for a urine specimen to be stored in a refrigerator before being cultured?
What is the maximum recommended time for a urine specimen to be stored in a refrigerator before being cultured?
What is the primary reason for collecting blood cultures in pairs, one aerobic and one anaerobic?
What is the primary reason for collecting blood cultures in pairs, one aerobic and one anaerobic?
Which of these is NOT a precaution for handling specimens?
Which of these is NOT a precaution for handling specimens?
Which of the following conditions refers to the presence of bacteria in the bloodstream?
Which of the following conditions refers to the presence of bacteria in the bloodstream?
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What is the primary potential consequence of failing to refrigerate a urine specimen within 30 minutes of collection?
What is the primary potential consequence of failing to refrigerate a urine specimen within 30 minutes of collection?
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What is the purpose of performing a colony count in urine culture analysis?
What is the purpose of performing a colony count in urine culture analysis?
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Which of the following is NOT essential in a completed lab requisition?
Which of the following is NOT essential in a completed lab requisition?
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What is the primary reason that improper collection techniques can negatively impact the results of a culture?
What is the primary reason that improper collection techniques can negatively impact the results of a culture?
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Why is clean-catch, midstream urine (CCMS) collection preferred for diagnosing urinary tract infections?
Why is clean-catch, midstream urine (CCMS) collection preferred for diagnosing urinary tract infections?
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What is the significance of a colony count of 100,000 CFU/mL or higher in a urine culture?
What is the significance of a colony count of 100,000 CFU/mL or higher in a urine culture?
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Which of the following is NOT a method used for collecting urine specimens for UTI diagnosis?
Which of the following is NOT a method used for collecting urine specimens for UTI diagnosis?
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What is the primary purpose of antimicrobial susceptibility testing in diagnosing a UTI?
What is the primary purpose of antimicrobial susceptibility testing in diagnosing a UTI?
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What is the significance of finding two or more bacteria per 1,000 microscopic fields in a Gram-stained urine smear?
What is the significance of finding two or more bacteria per 1,000 microscopic fields in a Gram-stained urine smear?
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Why is it important to cleanse the urethral opening before collecting a clean-catch midstream urine sample?
Why is it important to cleanse the urethral opening before collecting a clean-catch midstream urine sample?
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Which of the following is a potential reason for a high colony count in a urine culture even if the patient does not have a UTI?
Which of the following is a potential reason for a high colony count in a urine culture even if the patient does not have a UTI?
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Why is suprapubic needle aspiration considered more invasive for collecting urine samples?
Why is suprapubic needle aspiration considered more invasive for collecting urine samples?
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Which of the following is an example of a semi-critical item?
Which of the following is an example of a semi-critical item?
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What is the main difference between medical asepsis and surgical asepsis?
What is the main difference between medical asepsis and surgical asepsis?
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What is the primary purpose of Standard Precautions?
What is the primary purpose of Standard Precautions?
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Which of the following is NOT a requirement for surgical asepsis?
Which of the following is NOT a requirement for surgical asepsis?
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Which of these is the most effective method for preventing the spread of infections?
Which of these is the most effective method for preventing the spread of infections?
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According to the OSHA Bloodborne Pathogen Standard, which of the following topics must be included in a written Exposure Control Plan?
According to the OSHA Bloodborne Pathogen Standard, which of the following topics must be included in a written Exposure Control Plan?
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Which of these is NOT a bloodborne illness covered by the OSHA Bloodborne Pathogen Standard?
Which of these is NOT a bloodborne illness covered by the OSHA Bloodborne Pathogen Standard?
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Which of the following procedures requires the use of surgical asepsis?
Which of the following procedures requires the use of surgical asepsis?
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What is the primary method of infection prevention emphasized?
What is the primary method of infection prevention emphasized?
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What should be done before and after patient contact to prevent infection?
What should be done before and after patient contact to prevent infection?
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In which scenario are alcohol-based hand rubs recommended?
In which scenario are alcohol-based hand rubs recommended?
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What type of rooms are used for immunocompromised patients to prevent airborne infections?
What type of rooms are used for immunocompromised patients to prevent airborne infections?
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What is NOT recommended for healthcare personnel during patient care?
What is NOT recommended for healthcare personnel during patient care?
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Which of the following is NOT a general precaution for specimen collection and transport?
Which of the following is NOT a general precaution for specimen collection and transport?
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When is it ideal to collect a specimen for an infectious disease diagnosis, if possible?
When is it ideal to collect a specimen for an infectious disease diagnosis, if possible?
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What is the PRIMARY role of healthcare professionals in specimen collection and transport?
What is the PRIMARY role of healthcare professionals in specimen collection and transport?
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Which of the following statements accurately reflects the importance of specimen quality in infectious disease diagnosis?
Which of the following statements accurately reflects the importance of specimen quality in infectious disease diagnosis?
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Why is it important to collect specimens aseptically?
Why is it important to collect specimens aseptically?
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What is the role of laboratory procedure manuals (P&P manuals) in specimen handling?
What is the role of laboratory procedure manuals (P&P manuals) in specimen handling?
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Which of the following is NOT a factor that influences the selection of a clinical specimen type to diagnose an infection?
Which of the following is NOT a factor that influences the selection of a clinical specimen type to diagnose an infection?
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How can a healthcare professional ensure proper communication with a microbiology lab about a specimen?
How can a healthcare professional ensure proper communication with a microbiology lab about a specimen?
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What is the rate of Central Line-Associated Bloodstream Infections (CLABSI) in pediatric ICUs?
What is the rate of Central Line-Associated Bloodstream Infections (CLABSI) in pediatric ICUs?
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Which of the following is NOT a category of Healthcare-Associated Infections (HAIs)?
Which of the following is NOT a category of Healthcare-Associated Infections (HAIs)?
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What is the main reason for the importance of standardized procedures in healthcare settings?
What is the main reason for the importance of standardized procedures in healthcare settings?
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What is the predominant pathogen associated with Healthcare-Associated Infections (HAIs)?
What is the predominant pathogen associated with Healthcare-Associated Infections (HAIs)?
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Which of the following is a key element in effectively implementing procedures to prevent HAIs?
Which of the following is a key element in effectively implementing procedures to prevent HAIs?
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What is the reported rate of Catheter-Associated Urinary Tract Infections (CAUTI) in pediatric ICUs?
What is the reported rate of Catheter-Associated Urinary Tract Infections (CAUTI) in pediatric ICUs?
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Which of the following is NOT considered a healthcare setting?
Which of the following is NOT considered a healthcare setting?
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Flashcards
Clinical Specimens
Clinical Specimens
Samples like blood or urine collected for infection diagnosis.
Specimen Quality
Specimen Quality
Refers to the integrity and correctness of collected specimens.
Specimen Selection
Specimen Selection
Choosing the correct type of specimen for testing.
Aseptic Collection
Aseptic Collection
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Transport Procedures
Transport Procedures
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Collaboration with Microbiology Lab
Collaboration with Microbiology Lab
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Timing of Collection
Timing of Collection
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Sterile Containers
Sterile Containers
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Specimen Handling Precautions
Specimen Handling Precautions
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Transport Specimens Promptly
Transport Specimens Promptly
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Protect from Heat, Cold, Oxygen
Protect from Heat, Cold, Oxygen
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Label Specimens Correctly
Label Specimens Correctly
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Prevent Contamination
Prevent Contamination
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Urine Specimen Processing Time
Urine Specimen Processing Time
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Bacteremia
Bacteremia
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Urine Culture Analysis
Urine Culture Analysis
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Aseptic Technique
Aseptic Technique
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Colony-Forming Units (CFU)
Colony-Forming Units (CFU)
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Clean-Catch, Midstream Urine (CCMS)
Clean-Catch, Midstream Urine (CCMS)
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Indication of Urinary Tract Infection (UTI)
Indication of Urinary Tract Infection (UTI)
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Specimen Contamination
Specimen Contamination
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Antimicrobial Susceptibility Testing
Antimicrobial Susceptibility Testing
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Gram-Stained Urine Smear
Gram-Stained Urine Smear
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Microscopic Field Count
Microscopic Field Count
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Semi-critical Items
Semi-critical Items
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Noncritical Items
Noncritical Items
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Medical Asepsis
Medical Asepsis
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Surgical Asepsis
Surgical Asepsis
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Universal Precautions
Universal Precautions
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Bloodborne Pathogens
Bloodborne Pathogens
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Standard Precautions
Standard Precautions
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Hand Hygiene
Hand Hygiene
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Device-Associated Infections
Device-Associated Infections
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Ventilator-Associated Pneumonia (VAP)
Ventilator-Associated Pneumonia (VAP)
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Central Line-Associated Bloodstream Infections (CLABSI)
Central Line-Associated Bloodstream Infections (CLABSI)
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Catheter-Associated Urinary Tract Infections (CAUTI)
Catheter-Associated Urinary Tract Infections (CAUTI)
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Healthcare-Associated Infections (HAIs)
Healthcare-Associated Infections (HAIs)
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Gram-Positive Cocci
Gram-Positive Cocci
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Contact Transmission
Contact Transmission
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Preventable Infections
Preventable Infections
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Infection Prevention Method
Infection Prevention Method
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Antimicrobial Agents
Antimicrobial Agents
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Airborne Infection Isolation Rooms (AIIRs)
Airborne Infection Isolation Rooms (AIIRs)
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Recommended Vaccines
Recommended Vaccines
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Study Notes
Chapter 13: Diagnosing Infectious Diseases
- Accurate diagnosis of infectious diseases relies on patient history, physical examination, symptom evaluation, and proper specimen handling.
- Clinical specimens, such as blood, urine, and feces, are collected to diagnose or monitor infections.
- High-quality specimens are crucial for accurate lab results.
- Healthcare professionals play a vital role in specimen collection and transport.
Importance of High-Quality Specimens
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Specimen quality directly impacts the accuracy of lab results.
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Proper specimen selection, collection, and transport are required for high-quality specimens.
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Lab procedures and guidelines are essential to ensure correct handling of specimens.
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Proper Selection, Collection, and Transport
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Select the correct specimen type.
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Collect aseptically to minimize contamination.
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Collect from the site most likely to contain the pathogen.
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Collect before antimicrobial therapy begins, if possible.
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Collect during the acute stage of the disease.
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Collect sufficient specimen quantity.
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Use sterile containers for transport and storage
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Protect specimens from heat, cold, and oxygen, if needed.
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Label specimens correctly and include a completed lab requisition.
Role of Healthcare Professionals
- Close collaboration between clinicians and the microbiology lab is essential.
- Healthcare professionals select, collect, and transport specimens, and communicate findings to clinicians.
- Safety precautions (Standard Precautions) must be strictly followed during specimen handling.
Types of Clinical Specimens
- Blood: Sterile within the body; bacteremia (bacteria in the bloodstream), septicemia (serious disease with bacteria/toxins in bloodstream). Blood cultures are typically collected in pairs. Aseptic technique is crucial
- Urine: Normally sterile within the bladder, contamination can occur during urination due to contact with the distal urethra.
- Sputum: Collected from patients with respiratory infections (pneumonia, tuberculosis).
- CSF (Cerebrospinal Fluid): Obtained via lumbar puncture, crucial for diagnosing meningitis, encephalitis, and meningoencephalitis. Treated as a STAT specimen. Do not refrigerate.
- Fecal Specimens: Collected and processed promptly to prevent pH changes.
- Throat Swabs: Primarily for diagnosing strep throat (Streptococcus pyogenes).
- Wound Specimens: Aspirates are preferred over swabs for minimizing contamination.
Clinical Microbiology Laboratory (CML)
- Responsible for diagnosing infectious diseases.
- Responsibilities: Process samples, isolate pathogens, identify pathogens, antimicrobial susceptibility testing.
- Sections: Bacteriology, Mycology, Parasitology, Virology, Mycobacteriology.
Virology
- Diagnoses viral infections, uses techniques like immunodiagnostic tests, electron microscopy, cell culture, and molecular methods.
Mycobacteriology
- Focuses on tuberculosis diagnosis, uses acid-fast staining, culture, and biochemical tests.
Pathology Department
- Divided into Anatomical (autopsies, tissue exam, cytology) and Clinical Pathology (CML, Clinical Chemistry, Hematology, Blood Bank, Immunology).
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Description
This quiz covers the essential elements of diagnosing infectious diseases, focusing on the importance of high-quality clinical specimens. Learn about the procedures for specimen selection, collection, and transport that are crucial for accurate lab results. Understand the role of healthcare professionals in the diagnostic process.