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Questions and Answers
What is the primary purpose of differential centrifugation?
What is the primary purpose of differential centrifugation?
Differential centrifugation is only used in biochemistry.
Differential centrifugation is only used in biochemistry.
False
What type of equipment is used in differential centrifugation?
What type of equipment is used in differential centrifugation?
Centrifuge
The sample to be separated can be a ______ or tissue suspension.
The sample to be separated can be a ______ or tissue suspension.
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Match the key components of differential centrifugation with their descriptions:
Match the key components of differential centrifugation with their descriptions:
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Study Notes
Centrifugation Techniques
- Centrifugation is a laboratory method utilizing centrifugal force for the separation of particles or components in a mixture based on density, size, shape, and viscosity.
- Widely applied in scientific fields such as biochemistry, chemistry, and biology.
- Various types of centrifugation techniques serve specific purposes.
Differential Centrifugation
- A key laboratory technique for separating cellular components or particles from complex mixtures by exploiting differences in size, shape, and density.
- Fundamental method in cell biology and biochemistry, essential for isolating and purifying cellular organelles and components.
Key Components of Differential Centrifugation
- Centrifuge: High-speed equipment generates strong centrifugal forces; modern types include tabletop, ultracentrifuges, and super-speed centrifuges.
- Sample: A homogenized cell or tissue suspension that contains a mixture of cellular components including organelles, proteins, nucleic acids, and other particles.
- Buffer: A suitable buffer is utilized to create an isotonic and stable environment for the sample during centrifugation.
Working Principle of Differential Centrifugation
- Sample Homogenization: Biological samples, such as tissues or cell cultures, are homogenized in a buffer to break open cells, releasing their contents into the solution and forming a heterogeneous mixture.
- Centrifugation: The homogenized sample is placed in centrifuge tubes and subjected to centrifugal force, which facilitates the separation of components based on their physical properties.
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Description
Test your knowledge of centrifugation techniques and their applications in separating components of mixtures based on density. This quiz covers various centrifugal methods and parameters used in laboratory settings.
