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Centrifugation Quiz
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Centrifugation Quiz

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Questions and Answers

What is the main principle behind centrifugation?

  • Separating particles based on their size and density using centrifugal force (correct)
  • Separating particles based on their viscosity and medium using centrifugal force
  • Separating particles based on their solubility and reactivity using centrifugal force
  • Separating particles based on their shape and color using centrifugal force
  • What is the name of the remaining liquid above the precipitate after centrifugation?

  • Sediment
  • Supernatant (correct)
  • Precipitant
  • Residue
  • What effect does increasing the effective gravitational force have on the separation of particles in a mixture?

  • Has no effect on the separation of particles
  • Causes random separation of particles
  • Accelerates the separation of particles (correct)
  • Slows down the separation of particles
  • How is the rate of centrifugation usually specified?

    <p>Angular velocity in revolutions per minute (RPM)</p> Signup and view all the answers

    What factor does the conversion between RPM and g depend on?

    <p>The radius of the centrifuge rotor</p> Signup and view all the answers

    What is the name of the particles that migrate away from the axis of the centrifuge during centrifugation?

    <p>Pellet</p> Signup and view all the answers

    In centrifugation, what does the rate of particle separation from a heterogeneous mixture depend on?

    <p>Particle size and density</p> Signup and view all the answers

    What is the name of the remaining liquid above the precipitate after centrifugation?

    <p>Supernatant</p> Signup and view all the answers

    What factor determines the conversion between revolutions per minute (RPM) and acceleration (g) in centrifugation?

    <p>Radius of the centrifuge rotor</p> Signup and view all the answers

    What effect does increasing the effective gravitational force have on the separation of particles in a mixture during centrifugation?

    <p>Accelerates particle separation</p> Signup and view all the answers

    Study Notes

    Centrifugation Principles

    • Principle involves using rotation to create a force that separates particles in a mixture based on density.
    • Higher density particles migrate toward the bottom, forming a pellet, while less dense particles remain suspended.

    Supernatant

    • The liquid remaining above the pellet after centrifugation is called the supernatant.

    Effective Gravitational Force

    • Increasing the effective gravitational force enhances the separation of particles, allowing denser particles to settle more quickly.

    Rate of Centrifugation

    • The rate of centrifugation is typically specified in revolutions per minute (RPM) or relative centrifugal force (RCF, expressed in g-force).

    RPM and g Conversion Factors

    • The conversion between RPM and g depends on the radius of the centrifuge rotor and the gravitational constant.

    Migrating Particles

    • Particles that move away from the axis of the centrifuge during centrifugation are referred to as sedimenting particles.

    Separation Rate Factors

    • Rate of particle separation in a heterogeneous mixture is influenced by factors such as particle size, shape, density, and the viscosity of the medium.

    Supernatant (Reiteration)

    • The term for the liquid above the precipitate after centrifugation is consistently referred to as supernatant.

    RPM to Acceleration Conversion

    • The conversion between revolutions per minute (RPM) and acceleration (g) in centrifugation is determined by the radius of the rotor and the characteristics of the particles being separated.

    Enhanced Separation

    • Increasing the effective gravitational force during centrifugation results in more efficient and faster particle separation from mixtures.

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    Description

    Test your knowledge of centrifugation with this quiz! Explore the principles and applications of this mechanical separation process in chemistry and biology. From understanding centrifugal force to optimizing rotor speed, challenge yourself with questions on how centrifugation is used to separate particles based on size, shape, density, and more.

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