Central Dogma and Definitions of Life

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Questions and Answers

What is the correct sequence of the central dogma?

  • DNA -> RNA -> protein (correct)
  • protein -> DNA -> RNA
  • RNA -> DNA -> protein
  • DNA -> protein -> RNA

Viruses replicate solely according to the Central Dogma.

False (B)

What determines the 3D structure of a protein?

Amino acid sequence

According to the chemical Darwinian definition, life is a self-sustained chemical system capable of undergoing ________ evolution.

<p>Darwinian</p> Signup and view all the answers

Match the following definitions or features with their corresponding concepts:

<p>Water = H2O Life = Self-sustained chemical system capable of evolution Protein function = Dependent on structure Central dogma = DNA -&gt; RNA -&gt; protein</p> Signup and view all the answers

What dilemma is mentioned regarding defining 'life'?

<p>Overlapping definitions with non-living entities (A), Lack of a precise theoretical identity (C)</p> Signup and view all the answers

The function of a protein is entirely independent of its structure.

<p>False (B)</p> Signup and view all the answers

What is one challenge faced in defining 'life'?

<p>Robust counter-examples to proposed definitions</p> Signup and view all the answers

Who discussed the last universal common ancestor and viruses?

<p>Patrick Forterre (B)</p> Signup and view all the answers

There is a universally accepted definition of 'life'.

<p>False (B)</p> Signup and view all the answers

What are two essential processes that qualify something as being alive?

<p>Growth and reproduction</p> Signup and view all the answers

The Viking mission in 1976 was related to the search for _____ life forms.

<p>new</p> Signup and view all the answers

Match the researcher with their area of expertise:

<p>Prof.Xu Peng = Prokaryotic viruses and CRISPR Dr.Giovanni Gallo = CRISPR as genome editing tools Dr.Monica Piochi = Geological features related to the origin of life Dr.Afonso Morgada Mota = Exobiology</p> Signup and view all the answers

What is a major topic discussed in the seminars selected by students?

<p>Personal research interests (A)</p> Signup and view all the answers

Metaviromics analysis is not part of the research lines.

<p>True (A)</p> Signup and view all the answers

What approach is used to study the origin of life according to Dr.Martina Aulitto?

<p>Top down approach</p> Signup and view all the answers

What does the Pyrolytic Release (PR) experiment primarily search for?

<p>Evidence of photosynthesis (C)</p> Signup and view all the answers

In the Labeled Release (LR) experiment, heterotrophic microorganisms release radioactive carbon dioxide as a byproduct.

<p>True (A)</p> Signup and view all the answers

What is the purpose of the gas chromatograph in the Gas Exchange (GEx) experiment?

<p>To analyze gases produced or consumed during microbial metabolism.</p> Signup and view all the answers

In the Labeled Release (LR) experiment, soil is mixed with a labeled nutrient medium containing __________.

<p>radioactive organic compounds</p> Signup and view all the answers

Match the following experiments with their primary focus:

<p>Pyrolytic Release (PR) = Evidence of photosynthesis Labeled Release (LR) = Heterotrophic microorganisms Gas Exchange (GEx) = Metabolic byproducts of microorganisms Control Experiment = Environmental conditions</p> Signup and view all the answers

Which gas is not typically analyzed in the Gas Exchange (GEx) experiment?

<p>Nitrogen (C)</p> Signup and view all the answers

The Gas Exchange (GEx) experiment is conducted under dry conditions.

<p>False (B)</p> Signup and view all the answers

What type of experiment is designed to check for metabolic activity in heterotrophic microorganisms?

<p>Labeled Release (LR) experiment</p> Signup and view all the answers

What does evolutionary creationism integrate?

<p>Belief in divine creation and scientific knowledge (C)</p> Signup and view all the answers

Special creation claims that natural processes can independently create and develop life.

<p>False (B)</p> Signup and view all the answers

What theory suggested that life regularly arose from non-living matter?

<p>spontaneous generation</p> Signup and view all the answers

Evolutionary creationism accepts the scientific age of the Earth and biological __________.

<p>evolution</p> Signup and view all the answers

Match the following concepts with their definitions:

<p>Special Creation = Life was created by divine intelligence Biblical Fundamentalism = Immutability of species since creation Evolutionary Creationism = Life initially created but then evolved Spontaneous Generation = Life arises from non-living matter</p> Signup and view all the answers

Which belief asserts that species were divinely created in their current forms?

<p>Special creation (C)</p> Signup and view all the answers

Evolutionary creationism denies the scientific basis for biological evolution.

<p>False (B)</p> Signup and view all the answers

What outside factor does special creation argue is necessary for producing life?

<p>living intelligence</p> Signup and view all the answers

What are white smokers primarily formed from?

<p>Barium, calcium, and silicon (B)</p> Signup and view all the answers

Black smokers are formed from the same substances as white smokers.

<p>False (B)</p> Signup and view all the answers

What is the temperature of the hot water that erupts from the vents?

<p>350°C</p> Signup and view all the answers

The water from the hydrothermal vents has a low pH, typically around ______.

<p>2-3</p> Signup and view all the answers

Match the following components with their corresponding features:

<p>CH4 = Methane gas Fe(II) = Iron in reduced form Mn(II) = Manganese in reduced form H2 = Molecular hydrogen</p> Signup and view all the answers

Which organisms are supported by chemotrophic bacteria in the deep-sea hydrothermal vent ecosystems?

<p>Tube worms and fish (B)</p> Signup and view all the answers

Hydrothermal vents have a stable environment that does not change.

<p>False (B)</p> Signup and view all the answers

What type of environment do organisms near hydrothermal vents adapt to?

<p>Harsh and temporary environments</p> Signup and view all the answers

What did Francesco Redi's experiment demonstrate about maggots?

<p>Maggots only appear in uncovered jars with flies. (C)</p> Signup and view all the answers

The theory of spontaneous generation was widely accepted until the 18th century.

<p>True (A)</p> Signup and view all the answers

Who conducted the famous swan-neck flask experiment?

<p>Louis Pasteur</p> Signup and view all the answers

According to the theory of spontaneous generation, life arises from __________.

<p>non-living things</p> Signup and view all the answers

Match the following scientists with their contributions to the study of spontaneous generation:

<p>Francesco Redi = Demonstrated maggots arise from flies Louis Pasteur = Conducted swan-neck flask experiment Aristotle = Posited early theories of life from non-living matter Lamarck = Described evolution as progression from simple to complex</p> Signup and view all the answers

Which of the following statements describes the concept of spontaneous generation?

<p>Simple organisms emerge from inanimate materials. (A)</p> Signup and view all the answers

Experiments disproving spontaneous generation were conducted only in the 20th century.

<p>False (B)</p> Signup and view all the answers

What did Lamarck describe about the evolution of life?

<p>Evolution as progression from simpler to more complex and advanced forms</p> Signup and view all the answers

Flashcards

Exobiology

Study of life outside of Earth, involving the search for extraterrestrial life.

What is life?

A set of basic characteristics that define what makes something 'alive'. It's not about a specific organism, but the shared features of all living systems.

Biogenesis

The study of the origin, evolution, and diversity of life on Earth.

Abiogenesis

The idea that life can arise from non-living matter.

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Last Universal Common Ancestor (LUCA)

The last universal common ancestor is the hypothetical single-celled organism from which all living organisms on Earth descended.

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Defining Life

A science based approach to understanding the scientific meaning of being alive. It investigates the essential characteristics that define life.

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Central Dogma

The central dogma describes the flow of genetic information from DNA to RNA to protein. DNA replication produces an identical copy of the DNA. Transcription copies the DNA sequence into RNA. Translation converts the RNA sequence into a protein.

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Protein Structure and Function

Proteins are the workhorses of cells. Their 3D structure is determined by the order of amino acids, and this structure dictates their function.

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Viral Replication

Viruses can have genomes made of DNA or RNA, and they use different strategies to replicate. Some viruses can even rewrite the rules of the central dogma!

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Chemical Darwinian Definition

A chemical system that can reproduce itself and evolve is the proposed definition of life by NASA. This encompasses the idea of self-sustainability and Darwinian selection.

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Unified Theory of Biology

The search for a unified theory of biology is key to understanding the nature of life. Just as water is defined by H2O, a theoretical framework for life is needed.

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Evolutionary Creationism

The belief that life was created by a divine force and then evolved through natural processes.

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Special Creation

The belief that life was created by a divine force and has remained unchanged since.

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Spontaneous Generation

The idea that life arises from non-living matter.

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Immutability of Species

The belief that species are unchanging and were directly created in their current form.

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Pyrolytic Release (PR) Experiment

The method of measuring the incorporation of radioactive carbon (specifically 14CO2) into organic molecules within a sample.

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Labeled Release (LR) Experiment

The method of measuring the release of radioactive carbon dioxide (14CO2) from metabolizing organic compounds in a sample.

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Pyrolysis

The process of heating a sample to release any newly formed organic molecules, which are then detected for radioactive carbon.

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Labeled Release (LR) Experiment

A type of experiment designed to investigate the presence and activity of heterotrophic microorganisms within an environment.

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Pyrolytic Release (PR) Experiment

A method used to detect the presence of photosynthetic organisms by measuring the incorporation of radioactive carbon dioxide (14CO2) into organic molecules.

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Gas Exchange (GEx) Experiment

The experiment designed to measure the gases produced or consumed by microbes under simulated wet conditions, providing information about their metabolic activity.

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Gas Chromatography

The technique used in the GEx Experiment to separate and identify different gases in a sample.

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Heterotrophic Microorganisms

Organisms that obtain energy by breaking down organic matter.

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Redi's Experiment

Francesco Redi's experiment in 1668 that challenged spontaneous generation. He used jars with meat, some covered and some exposed, to show that maggots only appeared in the uncovered jars where flies had access to lay eggs.

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Pasteur's Swan-Neck Experiment

Louis Pasteur's experiment in 1859 that further disproved spontaneous generation. He used a swan-neck flask to show that sterilized broth remained sterile unless exposed to dust from the air, confirming that life arises from existing life, not inanimate matter.

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Lamarck's Theory of Evolution

Jean-Baptiste Lamarck's theory that evolution proceeded in a linear fashion from simpler to more complex life forms. This theory is now considered outdated but was influential in its time.

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Spontaneous Generation of Maggots

An outdated theory that proposed that maggots were created spontaneously from decaying matter. This theory was disproven by Francesco Redi.

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Theory of Evolution

A theory of evolution that suggests that organisms change over time in order to adapt to their environment. This is the accepted theory of evolution today.

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What are hydrothermal vents?

Hydrothermal vents are openings in the ocean floor where heated water and dissolved minerals are released from the Earth's interior.

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What are black smokers?

Black smokers are tall chimney-like structures formed by the precipitation of dissolved minerals from hydrothermal vents, giving them a dark color.

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What are white smokers?

White smokers are chimneys formed from deposits of barium, calcium, and silicon, resulting in a white color.

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What are the characteristics of hydrothermal vent water?

The water that erupts from hydrothermal vents is highly acidic (pH 2-3) and rich in dissolved sulfide minerals like iron (Fe) and manganese (Mn).

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What life exists at hydrothermal vents?

Hydrothermal vents support a diverse ecosystem of chemotrophic bacteria, tube worms, arthropods, fish, and other benthic lifeforms adapted to these harsh environments.

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What role do chemotrophic bacteria play at hydrothermal vents?

Chemotrophic bacteria use sulfur compounds as a source of energy, forming the base of the food chain in hydrothermal vent ecosystems.

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Where do hydrothermal vents occur?

Hydrothermal vents occur in areas where tectonic plates are moving apart, allowing magma to rise and heat seawater.

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Why are hydrothermal vents important?

Hydrothermal vents play a critical role in the global biogeochemical cycles, influencing the distribution of elements like carbon, sulfur, and metals.

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Study Notes

Emergence of Life and Exobiology

Objectives

  • Provide knowledge of the synergistic role of biotic and abiotic factors influencing the origin of Life on Earth.
  • Describe the state-of-the-art in experimental approaches for searching extraterrestrial life forms.

Prerequisites

  • Knowledge of microbiology, biochemistry, molecular biology, and chemistry.

Teaching materials

  • The Origins and Search for Life (English Edition) by Edward Trifonov, Nick Lane, Stephen Freeland, Michael Russell
  • Biosignatures for Astrobiology, edited by Cavalazzi, Barbara, and Westall, Frances.
  • Scientific papers and reviews.
  • Slides available for download from the professor's website.

Program

  • Definition of life.
  • Origin of life: introductory concepts, comparison of hypotheses (first metabolism, genes, vesicles).
  • Evolutionary relationships analysis
  • Extremophile viruses and the origin of life: isolation, identification of viruses and plasmids in extreme environments (metaviromics); virus-host interaction (CRISPR, manipulative genetics).
  • Role of genetic elements in horizontal gene transfer.
  • Analysis of viral genomes.
  • Identification of "core genes" within different viral genome families
  • Exobiology: concepts of biosignatures and planetary habitability; classification of biosignatures (gaseous, surface with temporal oscillations).
  • Introductory concepts of sample return (technical, temporal and procedural limitations).
  • Identification of sampling sites and methods.
  • Analysis of biological systems in extraterrestrial environments
  • Introductory concepts of synthetic life.

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