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Questions and Answers
Cytotoxicity assays measure the percentage of live cells.
Cytotoxicity assays measure the percentage of live cells.
False
MTT assay is a type of colorimetric assay.
MTT assay is a type of colorimetric assay.
True
In-vivo experiments are faster than cytotoxicity assays.
In-vivo experiments are faster than cytotoxicity assays.
False
Cytotoxicity assays are useful for testing a small number of samples.
Cytotoxicity assays are useful for testing a small number of samples.
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Apoptosis is a type of cell proliferation.
Apoptosis is a type of cell proliferation.
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Cytotoxicity assays are used to evaluate the therapeutic potential of a new drug under discovery.
Cytotoxicity assays are used to evaluate the therapeutic potential of a new drug under discovery.
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MTT assay is used to measure the dead cells over the live cells.
MTT assay is used to measure the dead cells over the live cells.
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Cytotoxicity assays have low predictability levels.
Cytotoxicity assays have low predictability levels.
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What is the primary purpose of a cytotoxicity assay?
What is the primary purpose of a cytotoxicity assay?
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What is a significant advantage of cytotoxicity assays compared to in-vivo experiments?
What is a significant advantage of cytotoxicity assays compared to in-vivo experiments?
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What is the primary application of MTT assays?
What is the primary application of MTT assays?
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What is a benefit of using cytotoxicity assays in drug discovery?
What is a benefit of using cytotoxicity assays in drug discovery?
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What is a type of assay that measures the loss of cellular or intercellular structure and/or functions?
What is a type of assay that measures the loss of cellular or intercellular structure and/or functions?
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What is the purpose of evaluating the safety profile of potential drugs?
What is the purpose of evaluating the safety profile of potential drugs?
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What is the advantage of using cytotoxicity assays for testing large numbers of samples?
What is the advantage of using cytotoxicity assays for testing large numbers of samples?
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What is a type of cytotoxicity assay that uses a dye exclusion method?
What is a type of cytotoxicity assay that uses a dye exclusion method?
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Study Notes
Cell Viability and Cytotoxicity Assays
- Cytotoxicity assays measure the effect of a lead compound on normal cell lines to ensure it does not harm human cells while killing pathogens.
- These assays evaluate the loss of cellular or intercellular structure and function, including lethal cytotoxicity, to indicate potential cell and tissue injury.
Applications of Cytotoxicity Assays
- Evaluate the safety profile of potential drugs
- Evaluate the therapeutic potential of new drugs under discovery
- Evaluate the effectiveness of anticancer agents
- Investigate apoptosis-induced cell death
- Use in membrane integrity assays, cell proliferation assays, migration assays, and necrosis assays
Advantages of Cytotoxicity Assays
- Faster than in-vivo experiments
- Inexpensive
- Reproducible
- Useful for testing large numbers of samples
- High predictability levels
- Rapid screening of promising drug candidates by filtering out cytotoxic compounds at an early stage
- Testing variable conditions such as different concentrations or combinations of various agents
Types of Cytotoxicity Assays
Dye Exclusion Assays
- Measure cell viability by excluding dead cells that are permeable to dyes
Colorimetric Assays
- MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)
- MTT assay determines cytotoxicity by distinguishing between live and dead cells
Cell Viability and Cytotoxicity Assays
- Cytotoxicity assays measure the effect of a lead compound on normal cell lines to ensure it does not harm human cells while killing pathogens.
- These assays evaluate the loss of cellular or intercellular structure and function, including lethal cytotoxicity, to indicate potential cell and tissue injury.
Applications of Cytotoxicity Assays
- Evaluate the safety profile of potential drugs
- Evaluate the therapeutic potential of new drugs under discovery
- Evaluate the effectiveness of anticancer agents
- Investigate apoptosis-induced cell death
- Use in membrane integrity assays, cell proliferation assays, migration assays, and necrosis assays
Advantages of Cytotoxicity Assays
- Faster than in-vivo experiments
- Inexpensive
- Reproducible
- Useful for testing large numbers of samples
- High predictability levels
- Rapid screening of promising drug candidates by filtering out cytotoxic compounds at an early stage
- Testing variable conditions such as different concentrations or combinations of various agents
Types of Cytotoxicity Assays
Dye Exclusion Assays
- Measure cell viability by excluding dead cells that are permeable to dyes
Colorimetric Assays
- MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)
- MTT assay determines cytotoxicity by distinguishing between live and dead cells
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Description
This quiz covers the concepts of cytotoxicity assays, cell viability, and apoptosis in relation to drug development and human cell lines.
