Campbell Bio 9+10(energy)

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Questions and Answers

What is the main difference between fermentation and aerobic respiration?

  • Fermentation occurs with oxygen, while aerobic respiration does not.
  • Fermentation produces more ATP than aerobic respiration.
  • Fermentation is more efficient than aerobic respiration.
  • Fermentation occurs without oxygen, while aerobic respiration requires oxygen. (correct)

In the context of aerobic respiration, what does the equation C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy represent?

  • The process of photosynthesis in plants.
  • The combustion of carbohydrates in the presence of oxygen. (correct)
  • The production of heat energy only.
  • The synthesis of glucose from carbon dioxide.

Which of the following correctly defines oxidation in the context of redox reactions?

  • A process where electrons are lost. (correct)
  • A process that increases the electronegativity of an atom.
  • A process involving the complete transfer of electrons.
  • A process where electrons are acquired.

What is true about the reducing agent in a redox reaction?

<p>It is oxidized during the reaction. (A)</p> Signup and view all the answers

Why is less energy required to extract electrons from less electronegative atoms?

<p>Because they hold their electrons less tightly. (A)</p> Signup and view all the answers

How does aerobic respiration compare to combustion of gasoline?

<p>Aerobic respiration is more efficient than combustion of gasoline. (B)</p> Signup and view all the answers

Which type of respiration occurs more rarely than aerobic respiration?

<p>Anaerobic respiration. (A)</p> Signup and view all the answers

What happens to an electron's potential energy when it moves closer to a more electronegative atom?

<p>It loses potential energy. (C)</p> Signup and view all the answers

What is the main reason hydrocarbons are considered an excellent energy source?

<p>They contain a high concentration of electrons capable of transferring to oxygen. (D)</p> Signup and view all the answers

What role does NAD+ play in cellular respiration during glucose breakdown?

<p>It acts as an oxidizing agent, accepting electrons. (D)</p> Signup and view all the answers

Which step of cellular respiration primarily occurs in the cytoplasm?

<p>Glycolysis (B)</p> Signup and view all the answers

What occurs at the end of the electron transport chain?

<p>O2 forms water by reacting with H+ ions. (C)</p> Signup and view all the answers

Why cannot energy be released all at once during cellular respiration?

<p>It would result in an explosion-like reaction. (A)</p> Signup and view all the answers

In the process of oxidative phosphorylation, which component is primarily responsible for ATP generation?

<p>Energy from the electron transport chain. (D)</p> Signup and view all the answers

What is the role of dehydrogenase in cellular respiration?

<p>To remove hydrogen atoms from glucose and convert NAD+ to NADH. (B)</p> Signup and view all the answers

During the citric acid cycle, what is produced alongside CO2?

<p>NADH and FADH2. (A)</p> Signup and view all the answers

How is energy harvested during the electron transport chain?

<p>Through the gradual transfer of electrons with associated energy release. (A)</p> Signup and view all the answers

What important characteristic of NAD+ makes it suited as an electron carrier?

<p>It can easily switch between oxidized and reduced forms. (B)</p> Signup and view all the answers

What distinguishes substrate level phosphorylation from oxidative phosphorylation?

<p>Transfers phosphate from a substrate molecule (A)</p> Signup and view all the answers

What is the primary role of pyruvate oxidation in cellular respiration?

<p>To convert pyruvate to acetyl CoA (B)</p> Signup and view all the answers

How many molecules of NADH are produced per pyruvate in the citric acid cycle?

<p>3 (D)</p> Signup and view all the answers

What is the purpose of GTP formed in step 5 of the citric acid cycle?

<p>It can be used to directly power work in cells (C)</p> Signup and view all the answers

Which process does NOT occur in the mitochondria?

<p>Glycolysis (C)</p> Signup and view all the answers

What does ATP synthase utilize to produce ATP?

<p>A pre-existing ion gradient (A)</p> Signup and view all the answers

Which of the following correctly describes the electron transport chain?

<p>Transfers electrons while using molecular oxygen as the final electron acceptor (C)</p> Signup and view all the answers

What is a defining characteristic of FADH2 in the electron transport chain?

<p>It donates electrons at a lower energy level than NADH (C)</p> Signup and view all the answers

Which component is NOT part of the electron transport chain?

<p>Citrate (C)</p> Signup and view all the answers

What type of reaction occurs as electrons are transferred between components in the electron transport chain?

<p>Alternating between reduction and oxidation (B)</p> Signup and view all the answers

What happens to the carbon atoms introduced in the citric acid cycle?

<p>Carbon atoms are completely lost as CO2 (C)</p> Signup and view all the answers

What is the main role of the multienzyme complex during pyruvate oxidation?

<p>To catalyze the oxidation of the pyruvate (D)</p> Signup and view all the answers

What is the significance of the structure of the inner mitochondrial membrane in relation to the electron transport chain?

<p>Its folding increases surface area for electron carriers (D)</p> Signup and view all the answers

What color light is least effective for photosynthesis according to the absorption spectrum of chlorophyll a?

<p>Green light (D)</p> Signup and view all the answers

What is the primary function of carotenoids in plants?

<p>To provide photoprotection (C)</p> Signup and view all the answers

Which component is NOT a part of a photosystem?

<p>Oxygen molecules (D)</p> Signup and view all the answers

What distinguishes chlorophyll a from chlorophyll b?

<p>Their color appearance under light (B)</p> Signup and view all the answers

What happens to an electron when a pigment absorbs a photon?

<p>It increases in potential energy (A)</p> Signup and view all the answers

Which of the following statements is true regarding the excited state of electrons?

<p>It typically reverts to the ground state rapidly. (B)</p> Signup and view all the answers

What tool is used to measure the absorption of light by pigments?

<p>Spectrophotometer (A)</p> Signup and view all the answers

What is the primary role of the reaction-center complex in a photosystem?

<p>To transfer electrons to a primary electron acceptor (D)</p> Signup and view all the answers

How does the action spectrum differ from the absorption spectrum?

<p>It profiles how certain colors impact the rate of photosynthesis. (D)</p> Signup and view all the answers

What phenomenon occurs when chlorophyll fluoresces?

<p>Release of photons in the visible spectrum (A)</p> Signup and view all the answers

Which process directly converts pyruvate into lactate?

<p>Lactic acid fermentation (C)</p> Signup and view all the answers

What is the primary reason for dough rising during baking?

<p>CO2 emissions from alcoholic fermentation (A)</p> Signup and view all the answers

Which of the following statements about lactate is correct?

<p>It can be recycled into glucose in the liver. (B)</p> Signup and view all the answers

What distinguishes obligate anaerobes from facultative anaerobes?

<p>Facultative anaerobes perform cellular respiration when oxygen is present. (A)</p> Signup and view all the answers

During which metabolic process is the majority of ATP produced?

<p>Oxidative phosphorylation (C)</p> Signup and view all the answers

What happens to excess lactate produced in white muscle cells during exercise?

<p>It is oxidized by red muscle cells. (C)</p> Signup and view all the answers

Which of the following statements about deamination is true?

<p>It creates nitrogenous waste as a byproduct. (B)</p> Signup and view all the answers

How does beta oxidation contribute to cellular respiration?

<p>It breaks down fatty acids into two-carbon fragments. (C)</p> Signup and view all the answers

Which of the following is a result of feedback inhibition?

<p>Excess product inhibits enzyme activity. (B)</p> Signup and view all the answers

What role does phosphofructokinase play in glycolysis?

<p>It regulates the speed of glycolysis. (C)</p> Signup and view all the answers

Which type of food molecule must undergo hydrolysis first before being processed in glycolysis?

<p>Carbohydrates (C)</p> Signup and view all the answers

Which of the following statements about glycolysis is correct?

<p>It can accept a variety of sugars, not just glucose. (B)</p> Signup and view all the answers

What characterizes the energy yield from one gram of fat compared to one gram of carbohydrate?

<p>Fats yield twice the amount of ATP as carbohydrates. (D)</p> Signup and view all the answers

What is a major component synthesized from dihydroxyacetone phosphate?

<p>Fat (B)</p> Signup and view all the answers

What is the primary role of the proton-motive force in cellular respiration?

<p>To drive ATP synthesis through the movement of protons across the membrane (C)</p> Signup and view all the answers

Which of the following statements about ATP production from glucose is accurate?

<p>The efficiency of converting glucose to ATP can vary based on cellular conditions. (C)</p> Signup and view all the answers

Why might hibernating mammals lower their metabolism rather than produce ATP?

<p>To conserve body temperature and energy stored in fat (B)</p> Signup and view all the answers

How does NADH contribute to ATP synthesis in the mitochondria?

<p>It donates electrons that pump protons across the mitochondrial membrane. (A)</p> Signup and view all the answers

In which scenario would only 1.5 ATP molecules be produced from FADH2?

<p>When FADH2 enters the electron transport chain early. (B)</p> Signup and view all the answers

What is the consequence of proton channels that allow protons to reenter the mitochondrial matrix without ATP synthase?

<p>They cause a decrease in ATP synthesis during hibernation. (D)</p> Signup and view all the answers

What distinguishes anaerobic respiration from fermentation?

<p>Anaerobic respiration uses electronegative molecules other than oxygen. (D)</p> Signup and view all the answers

Which product is generated during alcoholic fermentation?

<p>Ethanol (C)</p> Signup and view all the answers

What is the significance of Peter Mitchell's model of chemiosmosis?

<p>It provided a framework to explain oxidative phosphorylation. (D)</p> Signup and view all the answers

What efficiency percentage does the conversion of glucose to ATP typically achieve?

<p>34% (D)</p> Signup and view all the answers

What is the role of substrate-level phosphorylation in glycolysis?

<p>To directly produce ATP without the electron transport chain (A)</p> Signup and view all the answers

Which of the following correctly describes a process during anaerobic respiration?

<p>It does not require an electron transport chain. (B)</p> Signup and view all the answers

Which of these is a significant factor in determining ATP yield from glucose?

<p>The type of cell metabolism involved (B)</p> Signup and view all the answers

During which stage of cellular respiration is the majority of ATP produced?

<p>Electron Transport Chain (C)</p> Signup and view all the answers

What role does AMP play in phosphofructokinase activity?

<p>It stimulates phosphofructokinase activity. (D)</p> Signup and view all the answers

Which of the following correctly describes the structure of chloroplasts?

<p>They have a double membrane with thylakoids that create a third membrane system. (D)</p> Signup and view all the answers

What is the main product formed during the light reactions of photosynthesis?

<p>ATP and NADPH. (A)</p> Signup and view all the answers

What does the Calvin cycle utilize from the light reactions?

<p>NADPH and ATP. (B)</p> Signup and view all the answers

Why is Van Niel's conclusion about photosynthesis significant?

<p>It established that oxygen comes only from water splitting. (A)</p> Signup and view all the answers

What happens to electrons during the light reactions?

<p>They are transferred to NADP+ to form NADPH. (B)</p> Signup and view all the answers

What is the relationship between glucose and three-carbon sugars in photosynthesis?

<p>Three-carbon sugars are intermediates that can be converted to glucose. (A)</p> Signup and view all the answers

Which of the following pigments is crucial for capturing light energy during photosynthesis?

<p>Chlorophyll. (D)</p> Signup and view all the answers

How does the role of photosynthesis differ from cellular respiration?

<p>Photosynthesis releases oxygen while respiration consumes it. (C)</p> Signup and view all the answers

During which phase of photosynthesis is water split, providing electrons?

<p>Light reactions. (A)</p> Signup and view all the answers

What defines the wavelength of electromagnetic energy?

<p>The distance between crests of waves. (D)</p> Signup and view all the answers

What is a primary characteristic of visible light on the electromagnetic spectrum?

<p>It ranges from approximately 380-740 nm in wavelength. (C)</p> Signup and view all the answers

What is the purpose of chemiosmosis in photosynthesis?

<p>To synthesize ATP using a proton gradient. (B)</p> Signup and view all the answers

Which pigment is associated with the reaction center complex of photosystem II?

<p>P680 (B)</p> Signup and view all the answers

In the linear flow of electrons, what is the result of water splitting?

<p>Production of oxygen gas (A)</p> Signup and view all the answers

What is created in the thylakoid space as a result of the electron transport chain between PS II and PS I?

<p>A proton gradient (A)</p> Signup and view all the answers

Which process occurs only in photosystem I during the cyclic electron flow?

<p>Regeneration of P700 (D)</p> Signup and view all the answers

What is the main difference between linear electron flow and cyclic electron flow?

<p>One generates ATP while the other does not (A)</p> Signup and view all the answers

What role does NADP+ reductase play in photosynthesis?

<p>Reduction of NADP+ to NADPH (D)</p> Signup and view all the answers

How does the structure of P680 and P700 chlorophyll a differ?

<p>They are associated with different proteins (D)</p> Signup and view all the answers

What phenomenon explains the need for cyclic electron flow in some organisms?

<p>Evolutionary significance as a leftover process (C)</p> Signup and view all the answers

Which of the following statements about the thylakoid membrane is accurate?

<p>It houses photosystems II and I (A)</p> Signup and view all the answers

What does the energy from the proton motive force primarily contribute to?

<p>Synthesis of ATP through chemiosmosis (A)</p> Signup and view all the answers

Why might cyclic electron flow be considered photoprotective in some plants?

<p>It can prevent damage from excessive light (C)</p> Signup and view all the answers

What distinguishes the electron carriers in the thylakoid from those in the mitochondria?

<p>They have notable differences in photophosphorylation (C)</p> Signup and view all the answers

What is the primary role of PEP carboxylase in C4 plants?

<p>To fix CO2 to phosphoenolpyruvate (A)</p> Signup and view all the answers

How do C4 plants maintain a high concentration of CO2 in bundle-sheath cells?

<p>By pumping CO2 from mesophyll cells to bundle-sheath cells (D)</p> Signup and view all the answers

What is one hypothesized benefit of converting C3 plants to C4 plants in response to climate change?

<p>Enhanced resistance to high temperatures (C)</p> Signup and view all the answers

Why is photorespiration considered significant in plants?

<p>It can drain away a significant amount of fixed carbon (B)</p> Signup and view all the answers

In CAM plants, what is the purpose of stomata opening at night?

<p>To store CO2 as organic acids (C)</p> Signup and view all the answers

How do C4 plants avoid the negative effects of photorespiration?

<p>Through the energy investment of ATP to concentrate CO2 (D)</p> Signup and view all the answers

What is a key difference between the processes in C4 and CAM plants?

<p>C4 plants separate processes spatially while CAM plants do it temporally (B)</p> Signup and view all the answers

What role does cyclic electron flow play in bundle-sheath cells of C4 plants?

<p>It generates ATP to replace energy loss during the Calvin cycle (D)</p> Signup and view all the answers

Which plant type is less likely to be affected by rising temperatures and water constraints due to climate change?

<p>C4 plants (D)</p> Signup and view all the answers

How is G3P (glyceraldehyde 3-phosphate) used in plants?

<p>It serves as a precursor for various organic compounds (D)</p> Signup and view all the answers

What distinguishes C4 plants from C3 plants in terms of energy expenditure?

<p>C4 plants invest in ATP to minimize carbon loss from photorespiration (B)</p> Signup and view all the answers

Which type of plant primarily transports carbohydrates in the form of sucrose?

<p>All types of plants (A)</p> Signup and view all the answers

What is the significance of C4 plants having evolved multiple times?

<p>It shows the adaptability of the C4 pathway in different environments (D)</p> Signup and view all the answers

What is the primary source of electrons for the electron transport chain (ETC) in chloroplasts?

<p>Water (A)</p> Signup and view all the answers

Which statement correctly describes the function of the proton pumps in chloroplasts compared to mitochondria?

<p>Chloroplasts pump protons into the thylakoid space, while mitochondria pump into the matrix. (C)</p> Signup and view all the answers

What is the net gain of glyceraldehyde 3-phosphate (G3P) for each cycle of the Calvin cycle?

<p>One molecule of G3P (D)</p> Signup and view all the answers

In the context of C3 plants, what occurs when the stomata close on hot, dry days?

<p>Decrease in CO2 availability (B)</p> Signup and view all the answers

Which of the following correctly describes photorespiration?

<p>It consumes oxygen and releases CO2 (D)</p> Signup and view all the answers

What compound does carbon enter the Calvin cycle as?

<p>Carbon dioxide (B)</p> Signup and view all the answers

During the reduction phase of the Calvin cycle, what happens to 1,3-bisphosphoglycerate?

<p>It is reduced to form glyceraldehyde 3-phosphate (D)</p> Signup and view all the answers

What is considered the most abundant protein in chloroplasts?

<p>RuBP carboxylase-oxygenase (rubisco) (C)</p> Signup and view all the answers

How many ATP and NADPH are consumed for the net synthesis of one G3P in the Calvin cycle?

<p>9 ATP and 6 NADPH (B)</p> Signup and view all the answers

Which compound is NOT produced as a by-product in the light reactions of photosynthesis?

<p>Glucose (D)</p> Signup and view all the answers

In which environment do C3 plants tend to experience photorespiration more frequently?

<p>High temperature and bright light environments (D)</p> Signup and view all the answers

What impact does photorespiration have on the Calvin cycle?

<p>Decreases efficiency of photosynthesis (C)</p> Signup and view all the answers

What is the characteristic of glyceraldehyde 3-phosphate (G3P) produced in the Calvin cycle?

<p>It contains three carbon atoms (C)</p> Signup and view all the answers

In the Calvin cycle, the regeneration of ribulose bisphosphate (RuBP) requires how many ATP?

<p>3 ATP (D)</p> Signup and view all the answers

Flashcards

Fermentation

The process of breaking down sugars and other organic fuels without using oxygen.

Aerobic Respiration

A more efficient form of energy production that requires oxygen. The process involves the breakdown of glucose to release energy.

Redox Reactions or Oxidation-Reduction Reactions

The chemical reactions that involve the transfer of electrons between reactants. These reactions play a vital role in energy production within the cell.

Oxidation

A chemical reaction where a molecule loses electrons. The molecule becomes more positive.

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Reduction

A chemical reaction where a molecule gains electrons. The molecule becomes more negative.

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Reducing Agent

A substance that loses electrons (donates) in a redox reaction.

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Oxidizing Agent

A substance that gains electrons in a redox reaction.

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Electronegativity

A measure of an atom’s ability to attract electrons in a chemical bond. Higher electronegativity means the atom strongly attracts electrons.

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Electron Transfer

The process by which electrons move from molecules with lower electronegativity to molecules with higher electronegativity, releasing energy in the process.

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NAD+

A coenzyme that acts as an electron carrier in cellular respiration. It accepts electrons from glucose and carries them to the electron transport chain, where they are used to generate ATP.

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Dehydrogenases

Enzymes that remove hydrogen atoms (electrons and protons) from molecules like glucose during cellular respiration.

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Electron Transport Chain

The process by which electron carriers like NADH transfer electrons to a series of protein complexes embedded in the inner mitochondrial membrane, producing a proton gradient that is ultimately used to generate ATP.

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Oxidative Phosphorylation

The process of generating ATP in a process linked to the electron transport chain. It involves the movement of protons across the mitochondrial membrane, creating a proton gradient that drives ATP production.

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Glycolysis

A series of chemical reactions that break down glucose into pyruvate, producing a small amount of ATP and NADH.

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Pyruvate Oxidation

The process where pyruvate (from glycolysis) is converted into acetyl-CoA, which then enters the citric acid cycle.

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Citric Acid Cycle

A cyclic series of chemical reactions that continue the breakdown of glucose, producing ATP, NADH, and FADH2.

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Substrate Level Phosphorylation

The synthesis of ATP in a process directly coupled with the breakdown of glucose, such as in glycolysis and the citric acid cycle. This method yields smaller amounts of ATP compared to oxidative phosphorylation.

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CO2

A molecule of carbon dioxide, which is produced as a by-product of cellular respiration.

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Acetyl CoA

A molecule essential for various metabolic processes. It carries an acetyl group, which is used in the citric acid cycle.

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Citric Acid Cycle (Krebs Cycle)

A series of chemical reactions that occur in the mitochondrial matrix, oxidizing acetyl-CoA to CO2 and generating ATP, NADH, and FADH2. This is a key cycle in cellular respiration.

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FADH2

A reduced form of flavin adenine dinucleotide. It carries high-energy electrons from the citric acid cycle to the electron transport chain.

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Electron Transport Chain (ETC)

A series of protein complexes embedded in the inner mitochondrial membrane that uses the energy from electron transport to create a proton gradient, powering ATP synthesis. This is the final stage of cellular respiration.

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Prosthetic Group

A non-protein component that attaches to proteins in the electron transport chain, aiding in enzyme function.

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Flavoprotein

A protein that contains a flavin mononucleotide prosthetic group. It is a key component of the electron transport chain, accepting electrons from NADH.

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Ubiquinone (Coenzyme Q, CoQ)

A hydrophobic electron carrier in the electron transport chain. It is not a protein and can move independently within the membrane.

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Cytochrome

A type of protein that carries electrons in the electron transport chain. They contain a heme group, which is similar to the heme group in hemoglobin.

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ATP Synthase

A large protein complex that uses the proton gradient created by the electron transport chain to produce ATP from ADP and inorganic phosphate.

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Chemiosmosis

The process by which ATP is synthesized using the energy from a proton gradient. This is the mechanism by which ATP synthase generates ATP.

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ATP Yield from Glucose Oxidation

The net yield of ATP from the complete oxidation of glucose in cellular respiration, including ATP produced via substrate-level phosphorylation and oxidative phosphorylation.

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Proton-Motive Force

The difference in H+ concentration across the mitochondrial membrane, providing potential energy for ATP production.

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Alcoholic Fermentation

A type of anaerobic respiration where electrons are transferred to pyruvate, producing ethanol as a byproduct.

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Electron Shuttle

The process of using electron carriers like NADH and FADH2 to transport electrons from glycolysis to the mitochondria.

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ATP Yield

The estimated amount of ATP produced per glucose molecule under ideal conditions.

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Brown Fat

A type of tissue rich in mitochondria, specialized for heat production rather than ATP synthesis.

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Hibernation

A state of low metabolism and decreased body temperature, often exhibited by animals during winter.

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Proton-Motive Force (Work)

The use of energy from a proton gradient to perform cellular functions, like transporting molecules across the membrane.

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Lactic Acid Fermentation

A metabolic process that occurs in some bacteria and muscle cells, converting pyruvate to lactate without generating CO2.

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Deamination

The process where amino groups are removed from amino acids to form ammonia (NH3), which can be used for synthesis or excreted as waste.

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Beta Oxidation

The process by which fatty acids are broken down into acetyl CoA, generating ATP, NADH, and FADH2.

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Obligate Anaerobes

Organisms that can only survive in the absence of oxygen and rely on fermentation or anaerobic respiration to produce energy.

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Facultative Anaerobes

Organisms that can survive in both the presence and absence of oxygen. They can use fermentation or aerobic respiration to produce energy.

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Feedback Inhibition

A process of regulation that occurs when the end product of a metabolic pathway inhibits the activity of one or more of the enzymes involved in the pathway.

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Pyruvate Decarboxylation

A process that converts pyruvate into acetyl CoA, which then enters the citric acid cycle.

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Gluconeogenesis

A process where glucose is synthesized from non-carbohydrate precursors, such as pyruvate or lactate.

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Pigment Color

The color of a pigment is determined by the wavelengths of light it reflects. If a pigment absorbs all wavelengths, it appears black.

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Spectrophotometer

A spectrophotometer measures how much light a pigment absorbs at different wavelengths. It shines light through a solution of the pigment and measures how much light passes through, and thus, how much light is absorbed.

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Absorption Spectrum

A graph that shows how much light a pigment absorbs at different wavelengths. It's like a fingerprint for each pigment, showing its unique absorption pattern.

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Chlorophyll a

The main pigment involved in photosynthesis, it absorbs violet-blue and red light and is directly involved in the light reactions.

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Accessory Pigments

Pigments that help chlorophyll a absorb a wider range of light. They broaden the light spectrum available for photosynthesis.

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Action Spectrum

A graph that shows how effective different colors of light are at driving photosynthesis. It reveals the best colors for plant growth.

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Excited State

When a pigment absorbs light energy, its electrons jump to a higher energy level. This is like moving to a higher energy floor in a building.

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Energy Transfer

The process where energy absorbed by a pigment is passed from molecule to molecule until it reaches a special pair of chlorophyll a molecules in the reaction-center complex.

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Reaction-Center Complex

A collection of chlorophyll a molecules and a primary electron acceptor, which is the core of a photosystem.

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Electron Transfer in Photosynthesis

The process by which chlorophyll a, in the reaction-center complex, transfers an electron to a primary electron acceptor, initiating the light reactions. It is also a redox reaction.

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Phosphofructokinase

An enzyme that regulates glycolysis by controlling the rate of glucose breakdown. It is stimulated by AMP and inhibited by ATP, acting as a key control point for energy production.

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NADPH (Nicotinamide adenine dinucleotide phosphate)

An energy carrier molecule that is reduced by gaining electrons in the light-dependent reactions of photosynthesis, providing a source of high-energy electrons for the Calvin cycle.

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Chloroplast

The site of photosynthesis in plant cells. It contains chlorophyll and is organized into stacked membranes called thylakoids.

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Stroma

The fluid-filled space inside the chloroplast, where the Calvin cycle takes place. It is separated from the thylakoid space by the thylakoid membrane.

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Thylakoids

The stacked membrane structures within the chloroplast where the light-dependent reactions of photosynthesis occur. They contain chlorophyll and are separated from the stroma by the thylakoid membrane.

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Thylakoid space

The space inside the thylakoid membranes, where protons accumulate during the light-dependent reactions of photosynthesis.

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Light-dependent reactions

The process of converting light energy into chemical energy in the form of ATP and NADPH.

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Calvin cycle

The second stage of photosynthesis, where carbon dioxide is incorporated into organic molecules (sugars) using the ATP and NADPH generated in the light-dependent reactions.

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Carbon fixation

The incorporation of carbon dioxide into an organic molecule. It is the first step in the Calvin cycle and is essential for carbon fixation.

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Photophosphorylation

A type of phosphorylation that occurs during the light-dependent reactions of photosynthesis, where ATP is produced using the energy from a proton gradient across the thylakoid membrane.

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Mesophyll

The tissue inside the leaf where most chloroplasts are found, responsible for photosynthesis.

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Stomata

Pores on the surface of leaves that allow the entry of carbon dioxide and the exit of oxygen during photosynthesis.

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Endosymbiont theory

Theory proposing that chloroplasts and mitochondria were originally free-living prokaryotic organisms that were engulfed by eukaryotic cells and eventually became integrated as organelles.

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Electron Source in ETC

In chloroplasts, electrons for the ETC come from water, while in mitochondria they come from organic molecules carried by NADH and FADH2.

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ATP Production in Organelles

Chloroplasts convert light energy into ATP, while mitochondria convert energy from food molecules into ATP.

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Proton Pumping

Chloroplasts pump protons from the stroma into thylakoid space during the light-dependent reactions, while mitochondria pump protons from the matrix into the intermembrane space during oxidative phosphorylation.

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ATP Synthesis Direction

In chloroplasts, ATP is synthesized as protons flow from the thylakoid space to the stroma, opposite to the direction in mitochondria where protons flow from the intermembrane space to the matrix.

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Proton Gradient

The thylakoid membrane maintains a huge difference in proton concentration between the thylakoid space and the stroma, reaching a pH difference of 3, meaning a thousand-fold difference.

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NADPH Location

NADPH is produced in the stroma of chloroplasts, where the Calvin cycle takes place.

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Light-Driven Electron Flow

Light energy drives the electron flow from low potential energy state in water to high potential energy state in NADPH, also generating ATP.

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Calvin and Citric Acid Cycle Similarity

The Calvin cycle is similar to the citric acid cycle in that it recycles materials after a full cycle, but they differ in their metabolic function.

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Calvin and Citric Acid Cycle Function

The Calvin cycle is an anabolic process, using energy to build compounds, while the citric acid cycle is a catabolic process, breaking down compounds to produce energy.

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Calvin Cycle Input and Output

Carbon enters the Calvin cycle as CO2 and exits in the form of a sugar, consuming ATP and being reduced by NADPH.

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Calvin Cycle Product

The sugar produced by the Calvin cycle is not glucose but a three-carbon sugar called glyceraldehyde 3-phosphate (G3P).

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Calvin Cycle Turns

The Calvin cycle must complete three turns for the net synthesis of one molecule of G3P.

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Rubisco

Rubisco, the enzyme that catalyzes the first step of carbon fixation in the Calvin cycle, is the most abundant protein in chloroplasts and potentially in the world.

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Photorespiration

Photorespiration occurs when plants bind O2 instead of CO2 during the Calvin cycle, resulting in a two-carbon product and a release of CO2. This process consumes ATP and reduces photosynthetic output.

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C3 Plants

C3 plants, a majority of plants, produce 3-phosphoglycerate after carbon fixation in the Calvin cycle. They are less efficient in hot and dry conditions due to stomatal closure, leading to reduced CO2 levels and photorespiration.

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PEP Carboxylase

An enzyme found in mesophyll cells of C4 plants that binds CO2 with high affinity and zero O2 affinity.

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CAM (Crassulacean Acid Metabolism)

A process where plants like cacti and succulents open their stomata at night and close them during the day to conserve water and prevent CO2 loss.

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Bundle Sheath Cells

Specialized cells in C4 plants responsible for completing the Calvin cycle and providing a high concentration of CO2 for rubisco.

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Carbon Transfer in C4 Plants

The process of transferring carbon from mesophyll cells to bundle sheath cells, ensuring efficient carbon fixation in C4 plants.

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What are Photosystem I (PS I) and Photosystem II (PS II)?

Photosystem I and Photosystem II are protein complexes located in the thylakoid membrane involved in photosynthesis. They are named in the order they were discovered (not their function).

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What is P680?

The reaction-center complex in PS II has a chlorophyll a molecule (P680) that absorbs light best at 680 nm. This is crucial for the initial steps of electron transfer in photosynthesis.

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What is P700?

The reaction-center complex in PS I has a chlorophyll a molecule (P700) that absorbs light best at 700 nm. This is crucial for the later steps of electron transfer in photosynthesis.

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What is Linear Electron Flow?

The movement of electrons through photosystems and other membrane components (like the electron transport chain) during photosynthesis.

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Why is P680+ a strong oxidizing agent?

P680+ is a strong oxidizing agent because it has lost electrons. It has a high affinity for electrons, which allows it to strip electrons from water molecules, releasing oxygen as a byproduct.

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How are mitochondria and chloroplasts similar?

The electron carriers and ATP synthase present in mitochondria and chloroplasts share structural similarities, emphasizing their evolutionary connection.

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What happens in the electron transport chain between PS II and PS I?

The electron transport chain between PS II and PS I involves a series of redox reactions where electrons pass through plastoquinone (Pq), a cytochrome complex, and plastocyanin (Pc). This chain releases energy to pump protons into the thylakoid space.

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What is Chemiosmosis in photosynthesis?

Chemiosmosis is the process where ATP is synthesized using the energy from a proton gradient across the thylakoid membrane. This gradient is built up during the light reactions.

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What is Cyclic Electron Flow?

Cyclic electron flow is an alternative electron pathway in photosynthesis that occurs only in PS I. Electrons cycle back to ferredoxin (Fd), then to the cytochrome complex, and finally to plastocyanin (Pc) before returning to P700 in PS I.

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What is the possible function of cyclic electron flow?

Cyclic electron flow is thought to be a photoprotective mechanism, preventing damage to the photosynthetic machinery under high light conditions.

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How is cyclic electron flow important in bacteria with one photosystem?

Photosynthesizing bacteria that have only one photosystem depend on cyclic electron flow for ATP generation during photosynthesis. This is their only method for producing ATP.

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Describe the splitting of water in photosynthesis.

The excited electron from P680 in PS II is passed to an electron acceptor, forming P680+. This triggers the splitting of water, releasing oxygen, two protons into the thylakoid space, and electrons to replace the lost ones in P680.

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What is the role of NADP+ reductase in photosynthesis?

The enzyme NADP+ reductase catalyzes the transfer of electrons from ferredoxin (Fd) to NADP+, reducing it to NADPH. This process removes a proton from the stroma, which contributes to the proton gradient.

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What are the major components of the thylakoid membrane?

The thylakoid membrane houses two types of photosystems, PS II and PS I, which work together to capture light energy and convert it into chemical energy.

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What are the key components of a photosystem?

Each photosystem contains a reaction-center complex and a specific electron receiver next to a pair of chlorophyll a molecules. These components are crucial for light absorption and electron transfer.

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Study Notes

Energy and Cellular Processes

  • Organisms require energy for various functions, including polymer synthesis, active transport, movement, and reproduction
  • Energy enters and leaves systems (e.g., from light to heat), but biological chemicals are recycled
  • Organic compounds store potential energy, usable as fuels in exergonic reactions

Fermentation vs. Respiration

  • Fermentation is partial sugar breakdown without oxygen
  • Aerobic Respiration is more efficient and needs oxygen, common in eukaryotic and prokaryotic cells
  • Anaerobic Respiration uses reactants other than oxygen

Aerobic Respiration

  • Similar to gasoline combustion: Organic compound + Oxygen → Carbon dioxide + Water + Energy
  • Glucose breakdown: C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + Energy (ATP + Heat)
  • ∆G = -686 kcal/mol

Redox Reactions

  • Oxidation-reduction reactions (redox) involve electron transfer
  • Oxidation: Loss of electrons
  • Reduction: Gain of electrons (reduction because electrons are negatively charged)
  • Reducing agent: Donates electrons
  • Oxidizing agent: Gains electrons
  • Not all redox involve complete electron transfer; some change electron sharing degree (e.g., methane combustion)

Cellular Respiration Steps

  • Glycolysis: Occurs in the cytoplasm; glucose broken down to pyruvate; NAD⁺ reduced to NADH
  • Pyruvate Oxidation: Pyruvate converted to Acetyl CoA
  • Citric Acid Cycle (Krebs Cycle): Pyruvate further oxidized, yielding CO₂, ATP, NADH, and FADH₂

Oxidative Phosphorylation

  • Occurs in the inner mitochondrial membrane in eukaryotes
  • Substrate-level phosphorylation: Smaller amount of ATP formed in glycolysis and Krebs Cycle
  • Oxidative phosphorylation: Most ATP synthesis from electron transport chain (ETC) and chemiosmosis

Electron Transport Chain (ETC)

  • Series of proteins in the inner mitochondrial membrane
  • Electrons transferred from NADH and FADH₂, releasing energy to pump H⁺ into intermembrane space
  • Oxygen (highest electron affinity) is the final electron acceptor, forming water
  • Electron transfer from NADH to oxygen is exergonic (-53 kcal/mol)

Chemiosmosis

  • H⁺ gradient across membrane drives ATP synthesis via ATP synthase (a molecular motor)
  • Proton-motive force: H⁺ gradient's potential energy used to synthesize ATP
  • Prokaryotes use chemiosmosis to generate ATP and for other cellular functions (e.g., nutrient transport, flagella rotation)

ATP Yield and Efficiency

  • Complete glucose breakdown yields 30-32 ATP
  • ATP synthesis efficiency is about 34% of glucose's energy content
  • Excess energy released as heat

Fermentation

  • Anaerobic ATP production
  • Lactic acid fermentation: Pyruvate converted to lactate, regenerating NAD⁺
  • Alcoholic fermentation: Pyruvate converted to ethanol and CO₂, regenerating NAD⁺

Other Energy Sources

  • Carbohydrates, proteins, and fats are processed
  • Deamination: Amino groups removed from proteins used in other metabolic pathways
  • Beta-oxidation: Breakdown of fatty acids into acetyl-CoA
  • Fats yield twice the amount of ATP per gram compared to carbohydrates

Regulation of Cellular Respiration

  • Feedback inhibition: End product inhibits enzyme(e.g., ATP inhibits phosphofructokinase)
  • Phosphofructokinase: Key enzyme in glycolysis regulated by ATP levels:
  • Citrate: Additional regulator of glycolysis controlling early stages

Photosynthesis

Photosynthesis overview

  • Photosynthesis converts light energy to chemical energy (sugars): 6CO₂ + 6H₂O + Light Energy → C₆H₁₂O₆ + 6O₂
  • Oxygen released from water splitting
  • Chloroplasts are the sites of photosynthesis, with chlorophyll absorbing light
  • Photosynthesis involves two main stages: light reactions and the Calvin cycle

Light Reactions

  • Light energy converted to chemical energy (ATP and NADPH)
  • Water is split, releasing oxygen
  • Electron transfer through photosystems (PS II and PS I) via ETC
  • Chemiosmosis drives ATP synthesis during photophosphorylation
  • Light excites electrons, passing them through ETC components, producing ATP and NADPH
  • The flow of electrons and protons generates a proton gradient that is used to produce ATP using chemiosmosis

The Calvin Cycle

  • CO₂ incorporated into organic molecules (carbon fixation)
  • NADPH (reducing agent) and ATP supply energy for sugar synthesis
  • G3P (glyceraldehyde-3-phosphate): Three-carbon sugar produced
  • Rubisco is a key enzyme in carbon fixation, highly abundant
  • Three phases: carbon fixation, reduction, and regeneration

Photorespiration

  • Rubisco also binds with O₂ (photorespiration), decreasing sugar production
  • C₃ plants experience more photorespiration under high temperatures, lower CO₂ concentrations
  • C₄ plants and CAM plants have mechanisms to minimize photorespiration.

C₄ and CAM Plants

C4: -Spatial separation of carbon fixation and Calvin cycle CAM: -Temporal separation of carbon fixation and Calvin cycle (night vs. day)

Chlorophyll and Pigments

  • Chlorophyll absorbs light, mainly red and blue, reflecting green
  • Pigments like chlorophyll a and carotenoids broaden the range of light used for photosynthesis

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