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Questions and Answers

Which of the following is the correct order of the stages in aerobic respiration?

  • Krebs cycle, oxidative phosphorylation, glycolysis
  • Glycolysis, Krebs cycle, oxidative phosphorylation (correct)
  • Oxidative phosphorylation, Krebs cycle, glycolysis
  • Glycolysis, oxidative phosphorylation, Krebs cycle

Where does glycolysis take place in a cell?

  • Inner mitochondrial membrane
  • Nucleus
  • Mitochondrial matrix
  • Cytoplasm (correct)

What is the net gain of ATP molecules from glycolysis per glucose molecule?

  • 2 (correct)
  • 4
  • 38
  • 34

In the absence of oxygen, what process do muscle cells use to generate ATP during intense exercise?

<p>Fermentation (D)</p> Signup and view all the answers

Which of the following is NOT a product of the Krebs cycle?

<p>Pyruvate (A)</p> Signup and view all the answers

What is the primary role of oxygen in aerobic respiration?

<p>To act as the final electron acceptor in the electron transport chain (B)</p> Signup and view all the answers

Which process directly generates the most ATP during aerobic respiration?

<p>Oxidative Phosphorylation (A)</p> Signup and view all the answers

In what specific part of the mitochondria does oxidative phosphorylation occur?

<p>Inner mitochondrial membrane (A)</p> Signup and view all the answers

During anaerobic respiration in yeast, what two products are generated from glucose?

<p>Ethanol and carbon dioxide (B)</p> Signup and view all the answers

Why is aerobic respiration more efficient than anaerobic respiration?

<p>Aerobic respiration completely oxidizes glucose to carbon dioxide and water. (D)</p> Signup and view all the answers

What is the role of NADH and FADH2 in oxidative phosphorylation?

<p>They donate electrons to the electron transport chain. (C)</p> Signup and view all the answers

Which of the following is a direct consequence of lactic acid accumulation in muscle cells during intense exercise?

<p>Muscle fatigue and soreness (C)</p> Signup and view all the answers

If a scientist mutates a yeast strain such that it can no longer produce carbon dioxide during anaerobic respiration, what direct impact would this have on bread making?

<p>The dough would not rise properly. (C)</p> Signup and view all the answers

A researcher inhibits ATP synthase in a cell. What immediate effect would this have on cellular respiration?

<p>Reduced ATP production from the proton gradient (D)</p> Signup and view all the answers

Imagine a hypothetical scenario where a new, highly efficient organelle is discovered within a eukaryotic cell. This organelle enhances the electron transport chain by allowing protons to be pumped across the inner mitochondrial membrane at twice the normal rate using the same amount of electron carriers (NADH and FADH2). All other variables remain constant. How would this affect the overall ATP production in aerobic respiration in that cell, compared to a normal cell?

<p>ATP production would increase by less than double, limited by other factors in cellular respiration. (B)</p> Signup and view all the answers

During oxidative phosphorylation, what directly powers the synthesis of ATP by ATP synthase?

<p>The flow of protons down their electrochemical gradient (A)</p> Signup and view all the answers

Which stage of aerobic respiration does NOT directly produce ATP?

<p>Electron Transport Chain (C)</p> Signup and view all the answers

What is the fate of pyruvate produced during glycolysis under anaerobic conditions in muscle cells?

<p>It is converted to lactic acid. (B)</p> Signup and view all the answers

In the absence of oxygen, why must pyruvate be converted to lactate in muscle cells?

<p>To regenerate NAD+ for glycolysis to continue (B)</p> Signup and view all the answers

Which of the following statements accurately describes the role of FADH2 in aerobic respiration?

<p>It transports electrons to the electron transport chain. (C)</p> Signup and view all the answers

What is the primary purpose of setting up a control experiment with boiled seeds when investigating oxygen requirements in respiration?

<p>To confirm that any observed oxygen consumption is due to respiring organisms (B)</p> Signup and view all the answers

Why is carbon dioxide bubbled out of the water before starting an experiment to measure the rate of respiration of aquatic plants?

<p>To ensure all changes in carbon dioxide concentration are due to respiration (D)</p> Signup and view all the answers

Which of the following best explains why aerobic respiration is more efficient than anaerobic respiration?

<p>Aerobic respiration completely oxidizes glucose, extracting more energy. (D)</p> Signup and view all the answers

A scientist discovers a new drug that selectively inhibits the regeneration of NAD+ from NADH in muscle cells during anaerobic conditions. What direct effect would this drug have during intense exercise?

<p>Decreased ATP production due to the cessation of glycolysis (C)</p> Signup and view all the answers

In an experiment, a researcher uses a specific poison that inhibits the flow of electrons through the electron transport chain, but does not affect ATP synthase directly. What would be the immediate consequence within the mitochondria?

<p>A buildup of NADH and FADH2 (A)</p> Signup and view all the answers

During an investigation on cellular respiration, a group of students observes that a sealed container with germinating seeds shows a decrease in oxygen levels and an increase in carbon dioxide levels. If they introduce a chemical that completely halts the Krebs cycle, what would be the immediate effect on these gas levels within the container?

<p>Oxygen consumption would cease, and carbon dioxide production would decrease. (D)</p> Signup and view all the answers

If a scientist introduces a mutation into yeast cells that prevents them from producing ethanol during anaerobic respiration, but does not affect their ability to perform aerobic respiration, what would be a likely outcome when these cells are used in bread making?

<p>The bread would not rise properly due to the lack of CO2 production. (C)</p> Signup and view all the answers

A pharmaceutical company is developing a drug designed to enhance athletic performance by specifically targeting the efficiency of the electron transport chain in muscle cells. Which of the following mechanisms would most likely result in increased ATP production without causing detrimental side effects?

<p>A drug that facilitates the transfer of electrons from NADH and FADH2 to the electron transport chain, without affecting the proton gradient. (B)</p> Signup and view all the answers

Imagine a population of yeast cells is cultured in an environment that is initially aerobic but gradually becomes anaerobic. Over time, a mutation arises in one cell that allows it to efficiently convert lactic acid back into pyruvate. How would this mutation likely affect the survival and reproduction of this yeast cell compared to other cells in the increasingly anaerobic environment?

<p>It would increase its survival rate by allowing it to regenerate NAD+ more efficiently, thus sustaining glycolysis and ATP production. (B)</p> Signup and view all the answers

In a hypothetical scenario, a scientist discovers a novel bacterium capable of performing a modified version of the Krebs cycle, where each molecule of acetyl-CoA generates 5 molecules of FADH2 instead of the usual 1. Assuming that the electron transport chain and ATP synthase function with equivalent efficiency, how would the ATP yield per molecule of glucose compare between this novel bacterium and a typical organism that follows standard aerobic respiration?

<p>The novel bacterium would produce significantly more ATP, primarily due to a greater proton gradient generated by the electron transport chain. (B)</p> Signup and view all the answers

Which stage of aerobic respiration directly involves the consumption of oxygen?

<p>Oxidative Phosphorylation (D)</p> Signup and view all the answers

In which specific location within a eukaryotic cell does the Krebs cycle take place?

<p>Mitochondrial Matrix (D)</p> Signup and view all the answers

What is the primary role of NAD+ in glycolysis?

<p>To accept electrons and hydrogen ions (A)</p> Signup and view all the answers

How does the production of lactic acid allow glycolysis to continue under anaerobic conditions?

<p>It regenerates NAD+ from NADH, which is essential for glycolysis. (A)</p> Signup and view all the answers

Which of the following is NOT a reason why aerobic respiration is more efficient than anaerobic respiration?

<p>Aerobic respiration occurs in the cytoplasm, which is more efficient. (C)</p> Signup and view all the answers

During oxidative phosphorylation, what is the direct role of the proton gradient established across the inner mitochondrial membrane?

<p>To provide the energy for ATP synthase to function (C)</p> Signup and view all the answers

What would be the immediate effect on the Krebs cycle if a compound that inhibits the conversion of pyruvate to acetyl CoA were added to cells?

<p>The Krebs cycle would slow down or stop (D)</p> Signup and view all the answers

In an experiment testing CO2 production during respiration, why is it important to use a sealed container?

<p>To contain and measure the CO2 produced. (B)</p> Signup and view all the answers

How does the absence of oxygen affect the electron transport chain during aerobic respiration?

<p>It causes the electron transport chain to shut down. (D)</p> Signup and view all the answers

Why is a substance that absorbs $CO_2$ included in the setup when investigating the oxygen requirements for respiration using germinating seeds?

<p>To prevent the $CO_2$ from interfering with the measurement of oxygen consumption (C)</p> Signup and view all the answers

A scientist discovers a new organism that performs aerobic respiration but has a highly permeable inner mitochondrial membrane. How would this affect the organism's ATP production?

<p>ATP production would decrease. (C)</p> Signup and view all the answers

In a cell performing anaerobic respiration, what would be the impact of a drug that inhibits the enzyme responsible for converting pyruvate to lactic acid?

<p>Accumulation of pyruvate and a stall in glycolysis (A)</p> Signup and view all the answers

Consider a scenario where the enzyme ATP synthase is fully functional, but the protein complex responsible for pumping protons across the inner mitochondrial membrane is only 50% efficient. How would this directly impact the ATP yield from oxidative phosphorylation?

<p>ATP yield would decrease because fewer protons would be available to drive ATP synthesis. (D)</p> Signup and view all the answers

A newly discovered bacterial species thrives in an environment devoid of oxygen but is found to produce significantly more ATP per glucose molecule than other anaerobic organisms. Further investigation reveals that this bacterium utilizes a novel protein complex that efficiently transfers electrons from NADH directly to a metallic compound in the cell membrane, generating a proton gradient. How would this unique adaptation MOST directly enhance ATP production in this bacterium?

<p>By establishing a proton gradient without relying on traditional electron transport chain components, maximizing ATP production for anaerobic conditions. (B)</p> Signup and view all the answers

A researcher is investigating a mutant yeast strain that exhibits a unique metabolic behavior. When grown under anaerobic conditions, the mutant produces ethanol at a drastically reduced rate compared to wild-type yeast, yet its ATP production remains surprisingly robust. The researcher hypothesizes that the mutant yeast has evolved an alternative pathway to regenerate NAD+ from NADH. Which of the following metabolic products, if found in significantly higher concentrations in the mutant yeast compared to the wild-type under anaerobic conditions, would best support the researcher’s hypothesis?

<p>Glycerol (A)</p> Signup and view all the answers

Flashcards

Cellular Respiration

A metabolic process that converts biochemical energy from nutrients into ATP, releasing waste products.

Aerobic Respiration

The process requiring oxygen to produce energy from organic molecules, primarily glucose, through glycolysis, the Krebs cycle, and oxidative phosphorylation.

Glycolysis

The first stage of respiration, breaking down glucose into two pyruvate molecules, yielding a net gain of 2 ATP and 2 NADH.

Krebs Cycle

A cycle in the mitochondrial matrix that oxidizes acetyl CoA to carbon dioxide, producing 2 ATP, NADH, and FADH2 per glucose molecule.

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Oxidative Phosphorylation

A process on the inner mitochondrial membrane involving the electron transport chain and chemiosmosis, which uses NADH and FADH2 to create a proton gradient that drives ATP synthesis.

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Lactic Acid Fermentation

An anaerobic process where glucose is broken down into lactic acid, regenerating NAD+ and producing a small amount of ATP.

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Ethanol Fermentation

The anaerobic fermentation of sugars by yeast, producing ethanol, carbon dioxide, and ATP.

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Anaerobic Respiration

Respiration that does not require oxygen, yielding only 2 ATP molecules per glucose molecule.

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Aerobic Respiration

Respiration that requires oxygen, yielding approximately 38 ATP molecules per glucose molecule.

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Mitochondrial Matrix

This is the location of the Krebs cycle, a key stage in cellular respiration.

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Inner Mitochondrial Membrane

This is where oxidative phosphorylation occurs, using the electron transport chain and chemiosmosis to produce ATP.

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Objective of Oxygen Requirement Investigation

To determine if oxygen is required for respiration.

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Objective of CO2 Production Investigation

To verify that CO2 is a product of respiration.

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Beer Brewing

Yeast ferments sugars anaerobically, producing ethanol and carbon dioxide.

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Expected Outcome of Oxygen Investigation

Germinating seeds consume oxygen and produce CO2 during respiration.

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Glycolysis: Overview

First stage of both aerobic and anaerobic respiration occurring in the cytoplasm. Produces 2 ATP and 2 NADH.

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Pyruvate to Acetyl CoA

The conversion of pyruvate into acetyl CoA, which then enters the Krebs cycle for further oxidation.

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Oxidative Phosphorylation: Overview

Final stage of aerobic respiration, occurring on the inner mitochondrial membrane. Uses the electron transport chain and chemiosmosis to produce a large amount of ATP.

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Anaerobic Respiration in Muscles

Muscle cells switch from aerobic to anaerobic respiration producing lactic acid when oxygen supply is insufficient during intense exercise.

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Impact of Lactic Acid

Accumulation of lactic acid due to anaerobic respiration during exercise, leading to muscle fatigue and soreness.

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Bread Making

Yeast ferments sugars anaerobically to produce carbon dioxide, causing dough to rise and giving bread its soft texture.

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Location of Respiration

Aerobic process occurs in mitochondria, while anaerobic glycolysis occurs in the cytoplasm.

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End Products: Aerobic vs. Anaerobic

Aerobic respiration results in carbon dioxide and water; anaerobic respiration produces lactic acid in animals or ethanol and carbon dioxide in yeast.

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Total ATP Yield

The sum of ATP produced by glycolysis, Krebs cycle, and oxidative phosphorylation during aerobic respiration.

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Energy Production

The primary function of cellular respiration is creating ATP to fuel cellular activities.

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Waste Removal

Cellular respiration aids in the elimination of byproducts like carbon dioxide.

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Efficiency of Aerobic Respiration

Aerobic respiration generates significantly more energy per glucose molecule compared to anaerobic processes.

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Ethanol Production

Yeast fermentation of sugars anaerobically, yielding ethanol and carbon dioxide.

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Expected Outcome of CO2 Investigation

Expected result demonstrating respiration will release CO2, turning the lime water milky, showing the presence of carbon dioxide.

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Study Notes

  • Cellular respiration converts biochemical energy from nutrients into ATP, releasing waste products.
  • Aerobic respiration: glycolysis, Krebs cycle, and oxidative phosphorylation.

Overview of Aerobic Respiration

  • Aerobic respiration needs oxygen to produce energy from molecules like glucose.
  • It is more efficient than anaerobic respiration.
  • The three main stages are Glycolysis, Krebs Cycle, and Oxidative Phosphorylation.

Glycolysis

  • Location: Cytoplasm
  • First stage of both aerobic and anaerobic respiration.
  • Glycolysis converts one glucose molecule (6 carbons) into two pyruvate molecules (3 carbons each).
  • Net gain is two ATP molecules and two NADH molecules per glucose molecule.
  • Equation: Glucose (C6H12O6) + 2 NAD+ + 2 ADP + 2 Pi → 2 Pyruvate (C3H4O3) + 2 NADH + 2 ATP + 2 H2O

Krebs Cycle (Citric Acid Cycle)

  • Location: Mitochondrial Matrix
  • Pyruvate from glycolysis is oxidized into acetyl CoA.
  • Acetyl CoA enters the Krebs cycle and is fully oxidized to carbon dioxide.
  • Produces two ATP molecules per glucose molecule.
  • Also produces carriers of high-energy electrons (NADH and FADH2).
  • Equation: 2 Acetyl CoA + 6 NAD+ + 2 FAD + 2 ADP + 2 Pi → 4 CO2 + 6 NADH + 2 FADH2 + 2 ATP

Oxidative Phosphorylation

  • Location: Inner Mitochondrial Membrane
  • Involves the electron transport chain and chemiosmosis.
  • Electrons from NADH and FADH2 pass through proteins in the inner mitochondrial membrane.
  • Energy released during redox reactions pumps protons across the mitochondrial membrane, creating a gradient.
  • Protons flow back into the matrix through ATP synthase, driving ATP synthesis.
  • Equation: 10 NADH + 2 FADH2 + 34 ADP + 34 Pi + 6 O2 → 10 NAD+ + 2 FAD + 34 ATP + 12 H2O

Total ATP Yield

  • Glycolysis yields 2 ATP.
  • The Krebs cycle yields 2 ATP.
  • Oxidative phosphorylation yields approximately 34 ATP.
  • The total ATP yield per glucose molecule in aerobic respiration is approximately 38 ATP.

Significance

  • Energy Production: Primary role is to produce ATP for cellular processes.
  • Waste Removal: Helps remove metabolic waste products like carbon dioxide.
  • Efficiency: Aerobic respiration is more efficient than anaerobic, yielding more energy per glucose molecule.

Anaerobic Respiration in Muscle Cells During Exercise

  • During intense exercise, muscles switch from aerobic to anaerobic respiration due to limited oxygen.
  • Results in lactic acid production.
  • Chemical Equation: Glucose → Lactic Acid + ATP
  • Lactic Acid Formation: Glucose is broken down into lactic acid.
  • Regenerates NAD+ from NADH, allowing glycolysis to continue.
  • Impact on Muscles: Lactic acid accumulation can cause muscle fatigue and soreness.

Role of Anaerobic Respiration in Industry

  • Anaerobic respiration has applications in industries like brewing and breadmaking.
  • Beer Brewing: Yeast ferments sugars anaerobically, producing ethanol and carbon dioxide.
  • Necessary for alcohol content and carbonation of beer.
  • Equation: Glucose → Ethanol + CO2 + ATP
  • Bread Making: Yeast ferments sugars anaerobically to produce carbon dioxide.
  • This causes dough to rise, creating bread's soft texture.

Comparison Between Aerobic and Anaerobic Respiration

  • Oxygen Requirement: Aerobic requires oxygen, anaerobic does not.
  • ATP Yield: Aerobic produces up to 38 ATP, anaerobic yields only 2 ATP.
  • End Products: Aerobic results in carbon dioxide and water.
  • Anaerobic produces lactic acid (animals) or ethanol and carbon dioxide (yeast).
  • Location: Aerobic processes occur in mitochondria, anaerobic glycolysis occurs in the cytoplasm.

Investigations on Cellular Respiration

  • Oxygen Requirement in Respiration
  • Objective: To determine if oxygen is required for respiration.
  • Method: Use germinating seeds in a sealed container with a substance to absorb CO2.
  • A control setup without seeds or with boiled seeds to demonstrate the absence of respiration.
  • Expected Outcome: Live seeds consume oxygen and produce CO2, confirmed by a CO2 indicator turning cloudy.
  • CO2 Production During Respiration
  • Objective: To verify that CO2 is a product of respiration.
  • Method: Place organisms (small insects or microorganisms) in a closed environment with lime water.
  • Expected Outcome: Respiration by the organisms releases CO2, turning the lime water milky.

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