Cellular Metabolism and Energy Quiz
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Questions and Answers

In which cellular compartment does photophosphorylation occur?

  • Thylakoid membrane (correct)
  • Cytoplasm
  • Mitochondria
  • Nucleus
  • NAD+ is a reducing agent, meaning it accepts electrons from other molecules.

    False (B)

    What is the main function of NAD+ in cellular metabolism?

    Electron transfer

    NADH can pass the hydrogen atom to ______ in the cytoplasm to form lactate during lactate fermentation.

    <p>pyruvate</p> Signup and view all the answers

    Match the following processes with their corresponding products:

    <p>Lactate fermentation = Lactate Alcohol fermentation = Ethanol Photophosphorylation = ATP</p> Signup and view all the answers

    Which of the following is NOT an electron carrier used in cellular energy processes?

    <p>ATP (A)</p> Signup and view all the answers

    During respiration, the final electron acceptor is carbon dioxide.

    <p>False (B)</p> Signup and view all the answers

    What is the name of the molecule that serves as the energy currency of the cell?

    <p>ATP (Adenosine triphosphate)</p> Signup and view all the answers

    The process of photosynthesis uses light energy to convert carbon dioxide and water into ______ and oxygen.

    <p>glucose</p> Signup and view all the answers

    Match the following processes with their respective final electron acceptors:

    <p>Photosynthesis = CO2 Aerobic Respiration = O2 Methane Combustion = O2</p> Signup and view all the answers

    Which of the following processes releases energy?

    <p>ATP → ADP + Pi (C)</p> Signup and view all the answers

    In photosynthesis, the oxidation of water provides electrons for the reduction of carbon dioxide.

    <p>True (A)</p> Signup and view all the answers

    What are the two main types of energy harvesting processes in cells?

    <p>Photosynthesis and Respiration</p> Signup and view all the answers

    Which of the following is NOT a function of ATP?

    <p>Serving as a long-term energy storage molecule (C)</p> Signup and view all the answers

    ATP synthesis primarily occurs through the transfer of a phosphate group from ATP to ADP.

    <p>False (B)</p> Signup and view all the answers

    Name the enzyme responsible for the production of ATP in the cell.

    <p>ATP synthase</p> Signup and view all the answers

    The process of adding a phosphate group to a molecule is called ______.

    <p>phosphorylation</p> Signup and view all the answers

    Match the following types of phosphorylation with their respective descriptions:

    <p>Substrate-level phosphorylation = Involves the transfer of a phosphate group from a phosphorylated compound directly to ADP. Oxidative phosphorylation = Occurs in the inner membrane of mitochondria and is driven by a proton gradient. Photophosphorylation = Occurs in photosynthetic organisms and utilizes light energy to generate ATP.</p> Signup and view all the answers

    Which of the following molecules is the product of ATP hydrolysis?

    <p>ADP + Pi (D)</p> Signup and view all the answers

    Phosphorylation can either activate or inhibit an enzyme, depending on the specific enzyme and its phosphorylation site.

    <p>True (A)</p> Signup and view all the answers

    What is the primary source of energy released during ATP hydrolysis?

    <p>The breaking of the phosphate bond</p> Signup and view all the answers

    Which of the following molecules is the end product of glycolysis?

    <p>Pyruvate (C)</p> Signup and view all the answers

    Glycolysis requires oxygen to occur.

    <p>False (B)</p> Signup and view all the answers

    What is the net gain of ATP produced during glycolysis?

    <p>2 ATP</p> Signup and view all the answers

    The transfer of a phosphate group from PEP to ADP is an example of ______.

    <p>substrate-level phosphorylation</p> Signup and view all the answers

    Match the following molecules with their corresponding role in glycolysis:

    <p>Glucose = The starting molecule of glycolysis Pyruvate = The end product of glycolysis ATP = Energy currency used in glycolysis NADH = Electron carrier that stores energy PEP = A high-energy intermediate in glycolysis</p> Signup and view all the answers

    In the energy investment phase of glycolysis, how many ATP molecules are used?

    <p>2 (C)</p> Signup and view all the answers

    The citric acid cycle is a part of the energy payoff phase of glycolysis.

    <p>False (B)</p> Signup and view all the answers

    What is the name of the enzyme that catalyzes the conversion of pyruvate to lactate in anaerobic conditions?

    <p>Lactate dehydrogenase (LDH)</p> Signup and view all the answers

    During aerobic respiration, pyruvate is converted to ______ which enters the citric acid cycle.

    <p>Acetyl-CoA</p> Signup and view all the answers

    Match the following terms with their corresponding roles or products in glycolysis:

    <p>Glucose = The starting molecule for glycolysis Pyruvate = The end product of glycolysis ATP = The energy currency of the cell, used and produced in glycolysis NADH = An electron carrier molecule reduced during glycolysis</p> Signup and view all the answers

    In anaerobic conditions, yeast converts pyruvate to ______ and then to ______ through alcoholic fermentation.

    <p>acetaldehyde, ethanol</p> Signup and view all the answers

    The conversion of pyruvate to acetyl CoA occurs in the cytoplasm.

    <p>False (B)</p> Signup and view all the answers

    What is the primary function of the pyruvate dehydrogenase complex?

    <p>Converting pyruvate to acetyl CoA (B)</p> Signup and view all the answers

    What is the name of the coenzyme that combines with acetate to form acetyl CoA?

    <p>Coenzyme A</p> Signup and view all the answers

    Match the following molecules with their respective roles in glycolysis and pyruvate oxidation:

    <p>Pyruvate = The end product of glycolysis Acetyl CoA = The molecule that enters the Krebs cycle NAD+ = An electron carrier that is reduced to NADH CO2 = A byproduct of pyruvate oxidation</p> Signup and view all the answers

    Flashcards

    Electron Carriers

    Molecules that transport electrons during cellular processes.

    NADP+ to NADPH

    Conversion that occurs in photosynthesis, storing energy.

    NAD+ to NADH

    Conversion that occurs in respiration, capturing energy.

    Final Electron Acceptor

    Substance that receives electrons at the end of a process.

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    Oxidation

    Loss of electrons or hydrogen; releases energy.

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    Reduction

    Gain of electrons or hydrogen; stores energy.

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    ATP

    Adenosine Triphosphate, the main energy currency of the cell.

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    Methane Combustion

    A redox reaction where methane is oxidized, releasing energy.

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    Photophosphorylation

    A light-dependent reaction occurring in the thylakoid membrane of chloroplasts that generates ATP.

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    ATP Synthase

    An enzyme that synthesizes ATP using a proton gradient.

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    NAD+

    A coenzyme that acts as an oxidizing agent, accepting electrons in metabolic reactions.

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    NADH

    The reduced form of NAD+, which donates electrons and acts as a reducing agent in reactions.

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    Hydrolysis of ATP

    The process that breaks ATP into ADP and Pi, releasing energy.

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    Phosphorylation

    The addition of a phosphate group to a molecule, often from ATP.

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    Dehydrogenation

    A metabolic reaction where hydrogen atoms are removed from a molecule, often involving NAD+.

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    Lactate Fermentation

    A process where NADH donates hydrogen to pyruvate, forming lactate during anaerobic conditions.

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    Protein Kinases

    Enzymes that catalyze the phosphorylation of other proteins.

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    Dephosphorylation

    The removal of a phosphate group from a phosphorylated protein.

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    Substrate Level Phosphorylation

    Direct transfer of a phosphate group to ADP from a substrate.

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    Oxidative Phosphorylation

    A process that uses the electron transport chain and chemiosmosis to produce ATP.

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    Last reaction of glycolysis

    Transfers phosphate from PEP to ADP, producing ATP.

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    Net gain of ATP in glycolysis

    Glycolysis produces a total of 2 ATP after investment.

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    Energy investment phase

    The first part of glycolysis using 2 ATP.

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    Energy payoff phase

    Second part of glycolysis that produces 4 ATP.

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    NADH production in glycolysis

    Step 6 produces NADH used for ATP in oxidative phosphorylation.

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    End product of glycolysis

    Glycolysis breaks glucose down into two pyruvate molecules.

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    Pyruvate oxidation in aerobic conditions

    With oxygen, pyruvate enters citric acid cycle for energy extraction.

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    Glycolysis

    A metabolic pathway that converts glucose into pyruvate, producing ATP and NADH.

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    Net ATP gain in glycolysis

    Glycolysis generates a net of 2 ATP after consuming 2 ATP and producing 4 ATP.

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    Fate of pyruvate under aerobic conditions

    Pyruvate is converted to Acetyl-CoA, entering the TCA cycle for further energy extraction.

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    Fate of pyruvate under anaerobic conditions

    Pyruvate is converted to lactate, regenerating NAD+ in the absence of oxygen.

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    Lactate dehydrogenase (LDH)

    An enzyme that catalyzes the conversion of pyruvate to lactate.

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    Pyruvate dehydrogenase (PDH)

    An enzyme that converts pyruvate to Acetyl-CoA before entering the TCA cycle.

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    Anaerobic Glycolysis

    A metabolic process that converts glucose to energy without oxygen, producing pyruvate.

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    Alcoholic Fermentation

    A process where pyruvate is converted to ethanol and CO2 in anaerobic conditions, regenerating NAD+.

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    Pyruvate Decarboxylase

    An enzyme that catalyzes the conversion of pyruvate to acetaldehyde by removing CO2.

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    Link Reaction

    The process linking glycolysis to the Krebs cycle, converting pyruvate to acetyl CoA.

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    Acetyl CoA

    A molecule formed from pyruvate, which enters the Krebs cycle for further energy production.

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    Active Transport of Pyruvate

    The process requiring energy to move pyruvate into the mitochondria due to its charge.

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    Pyruvate Dehydrogenase Complex

    A multi-enzyme complex that catalyzes the conversion of pyruvate to acetyl CoA.

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    Study Notes

    Respiration

    • Living organisms obtain energy through respiration.
    • Respiration is a process of gaseous exchange, occurring at the respiratory surface.
    • Cellular respiration is a sequence of enzyme-controlled biochemical reactions that involve the breakdown of complex molecules. This process releases energy in the form of heat and potential energy which is used to produce ATP.

    Cellular Respiration

    • Metabolic pathways are sequences of reactions.

    • Different metabolic pathways have different types of reaction sequences.

      • Linear pathways: reactions proceed in a straight line, such as A -> B -> C -> D -> E.
      • Cyclic pathways: reactions form a cycle, with the starting compound being regenerated at the end.
      • Branched pathways: reactions diverge into several pathways.
    • Respiration can be either aerobic (with oxygen) or anaerobic (without oxygen).

    • The equation for aerobic respiration is: C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + ATP.

    Cellular Energy Harvest

    • Cells harvest energy by shifting electrons from one molecule to another.
      • Aerobic respiration: final electron acceptor is oxygen.
      • Anaerobic respiration: final electron acceptor is an inorganic molecule other than oxygen.
      • Fermentation: final electron acceptor is an organic molecule.
    • Energy carriers:
      • Pi (inorganic phosphate)
      • ATP
      • Electron carriers: NAD+, FAD

    Electron Carriers

    • Photosynthesis: NADP+ + H→ NADPH
    • Respiration:
      • NAD+ + H → NADH
      • FAD+ + 2H → FADH₂

    Final Electron Acceptor

    • Photosynthesis: CO₂ + H₂O → (CH₂O)ₙ + O₂
    • Respiration (Aerobic): ½ O₂ + H⁺ → H₂O

    ATP

    • Adenosine Triphosphate (ATP) is the energy currency of the cell.
    • ATP is used to drive cellular work, including movement and endergonic reactions.
    • ATP acts as a temporary energy store. Hydrolysis of ATP yields ADP and Pi, and releases 30.6 kJ of energy per mole of ATP.

    Phosphorylation

    • Production of ATP = phosphorylation (adding a phosphate group).
      • Occurs commonly by transferring phosphate from ATP to serine, threonine, or tyrosine residues in a protein.
      • Enzymes that catalyze the phosphorylation of other proteins are called protein kinases.
    • Dephosphorylation: removing a phosphate group (reversal of phosphorylation).
      • Enzymes that catalyze this are called protein phosphatases.

    Substrate-level Phosphorylation

    • A phosphate group is transferred directly from a phosphorylated compound to ADP.

    Oxidative Phosphorylation

    • Involves chemiosmosis and occurs in the inner mitochondrial membrane.
    • Produces the majority of ATP during cellular respiration.

    Photophosphorylation

    • Occurs during the light-dependent reactions in the thylakoid membrane.

    NAD⁺ & NADH

    • Nicotinamide adenine dinucleotide (NAD⁺) is a coenzyme found in all living cells.
    • NAD⁺ and NADH are involved in redox reactions, transferring electrons.

    FAD & FADH₂

    • Flavin adenine dinucleotide (FAD) is a redox cofactor essential for cellular respiration.
    • FAD accepts two electrons and two protons to become FADH₂.

    Glycolysis (Stage I)

    • Glycolysis is a metabolic pathway that converts glucose into two molecules of pyruvate.
    • It occurs in the cytoplasm and does not require oxygen.
    • Ten reactions comprise the pathway, producing two molecules of ATP and two molecules of NADH.

    Oxidation of Pyruvate (Stage II)

    • Pyruvate is transported into the mitochondria.
    • Decarboxylation: loss of CO₂ from pyruvate.
    • Oxidation: transfer of electrons from pyruvate to NAD+, forming NADH.
    • Pyruvate combines with coenzyme A (CoA) to create acetyl CoA.
    • Summary: 2 pyruvate + 2 CoA + 2 NAD⁺ → 2 acetyl CoA + 2 CO₂ + 2 NADH + 2 H⁺

    Krebs Cycle (Stage III)

    • Acetyl CoA enters the Krebs cycle.
    • A series of eight enzyme-catalyzed reactions in the mitochondrial matrix.
    • CO₂ is released, ATP is produced, and electrons are transferred to NADH and FADH₂.
    • A crucial part of aerobic respiration.

    Electron Transport Chain (Stage IV)

    • NADH and FADH₂ donate electrons to the electron transport chain.
    • Electrons are passed along a series of carrier molecules, with energy released used to pump protons (H⁺) across the membrane.
    • Chemiosmosis: energy from the proton gradient used to produce ATP, via ATP synthase.
    • Oxygen is the final electron acceptor, producing water.

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    Test your knowledge on cellular metabolism and energy processes with this quiz. From photophosphorylation to electron carriers, challenge yourself with questions that cover key concepts in cellular energy. Explore how molecules like NAD+ and NADH function in metabolic pathways.

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