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Questions and Answers
What is one potential application of gene editing in agriculture?
What is one potential application of gene editing in agriculture?
HIV can be transmitted through saliva.
HIV can be transmitted through saliva.
False
What does ART stand for in the context of HIV treatment?
What does ART stand for in the context of HIV treatment?
Antiretroviral therapy
HIV attacks the _____ cells, which are essential for the immune system's functioning.
HIV attacks the _____ cells, which are essential for the immune system's functioning.
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Match the following terms related to HIV with their descriptions:
Match the following terms related to HIV with their descriptions:
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What does the cell theory state about all living organisms?
What does the cell theory state about all living organisms?
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Spontaneous generation is a widely accepted and proven hypothesis in biology.
Spontaneous generation is a widely accepted and proven hypothesis in biology.
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What was the conclusion of Louis Pasteur's experiment regarding microorganisms?
What was the conclusion of Louis Pasteur's experiment regarding microorganisms?
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The __________ contains genetic material and controls cell activities.
The __________ contains genetic material and controls cell activities.
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Match the organelles with their functions:
Match the organelles with their functions:
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What determines the controlled variable in an experiment?
What determines the controlled variable in an experiment?
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Francesco Redi's experiment proved that maggots could arise spontaneously from meat.
Francesco Redi's experiment proved that maggots could arise spontaneously from meat.
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What is the role of lysosomes in a cell?
What is the role of lysosomes in a cell?
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What is the primary function of chloroplasts in plant cells?
What is the primary function of chloroplasts in plant cells?
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Centrosomes are involved in the breakdown of fatty acids.
Centrosomes are involved in the breakdown of fatty acids.
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What role do peroxisomes play in the cell?
What role do peroxisomes play in the cell?
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The ______ provides structure and rigidity to plant cells.
The ______ provides structure and rigidity to plant cells.
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Match the following components with their functions:
Match the following components with their functions:
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Which of the following is NOT one of the six points of the cell as an open system?
Which of the following is NOT one of the six points of the cell as an open system?
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Mitosis and meiosis are processes that enable cells to reproduce.
Mitosis and meiosis are processes that enable cells to reproduce.
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What is the manipulated variable in an experiment testing the effect of light intensity on plant growth?
What is the manipulated variable in an experiment testing the effect of light intensity on plant growth?
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HeLa cells are known to have a limited lifespan when grown in culture.
HeLa cells are known to have a limited lifespan when grown in culture.
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What organelle is responsible for digesting waste materials in animal cells?
What organelle is responsible for digesting waste materials in animal cells?
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The ______ provides structural support and protection in plant cells.
The ______ provides structural support and protection in plant cells.
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Which organelle produces energy (ATP) through cellular respiration?
Which organelle produces energy (ATP) through cellular respiration?
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Match the following organelles with their functions:
Match the following organelles with their functions:
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The large central vacuole in plant cells helps maintain turgor pressure.
The large central vacuole in plant cells helps maintain turgor pressure.
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What is a significant contribution of HeLa cells to medical research?
What is a significant contribution of HeLa cells to medical research?
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Which of the following is a characteristic of viruses?
Which of the following is a characteristic of viruses?
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Stem cells can only differentiate into one type of cell.
Stem cells can only differentiate into one type of cell.
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What type of stem cells are found in early-stage embryos?
What type of stem cells are found in early-stage embryos?
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CRISPR-Cas9 is a widely used _________ technology.
CRISPR-Cas9 is a widely used _________ technology.
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Which of the following statements about adult stem cells is true?
Which of the following statements about adult stem cells is true?
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Viruses can have an outer lipid envelope.
Viruses can have an outer lipid envelope.
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Name a virus that is an example of a virus discussed.
Name a virus that is an example of a virus discussed.
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Match the stem cell types with their descriptions:
Match the stem cell types with their descriptions:
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Which of the following best describes a responding (dependent) variable?
Which of the following best describes a responding (dependent) variable?
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The total magnification is calculated by multiplying the ocular lens magnification by the objective lens magnification.
The total magnification is calculated by multiplying the ocular lens magnification by the objective lens magnification.
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What is the formula for estimating specimen size using the field of view (FOV)?
What is the formula for estimating specimen size using the field of view (FOV)?
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In the formula A = I × M, A represents the _____ size.
In the formula A = I × M, A represents the _____ size.
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Match the type of cell with its characteristic:
Match the type of cell with its characteristic:
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If the image size is 200 µm and the magnification is 400x, what is the actual size of the specimen?
If the image size is 200 µm and the magnification is 400x, what is the actual size of the specimen?
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Prokaryotes are typically larger than eukaryotes.
Prokaryotes are typically larger than eukaryotes.
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The diameter of the field of view at 100x magnification is calculated using the formula M1D1 = M2D2. If D1 is _____ mm at 40x magnification, what is D2 at 100x magnification when M1 is 40x?
The diameter of the field of view at 100x magnification is calculated using the formula M1D1 = M2D2. If D1 is _____ mm at 40x magnification, what is D2 at 100x magnification when M1 is 40x?
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Study Notes
Cell Theory
- All living organisms are composed of one or more cells.
- The cell is the basic unit of structure and function in living organisms.
- All cells arise from pre-existing cells.
Spontaneous Generation
- Spontaneous generation is the outdated and disproven theory that living organisms can arise from non-living matter.
Pasteur's Experiment
- Pasteur conducted an experiment using swan-necked flasks to disprove spontaneous generation.
- He boiled broth to kill any existing microbes.
- Only when exposed to air (through the swan neck), did microbes grow.
- Conclusion: Microorganisms come from pre-existing microbes, not spontaneously from non-living matter.
Redi's Experiment
- Redi placed meat in jars, some covered and some uncovered.
- Maggots only appeared in the uncovered jars.
- Conclusion: Maggots come from fly eggs, not spontaneous generation. This disproved spontaneous generation for larger organisms.
Variable Definitions
- Controlled Variable: Factors kept constant in an experiment.
- Manipulated (Independent) Variable: Factor deliberately changed in an experiment.
- Responding (Dependent) Variable: Factor measured as a result of the manipulated variable's change.
Organelles (Plant and Animal Cells)
- Nucleus: Controls cell activities, contains DNA.
- Mitochondria: Produces energy (ATP) through cellular respiration.
- Ribosomes: Synthesize proteins.
-
Endoplasmic Reticulum (ER):
- Rough ER: Studded with ribosomes, involved in protein synthesis & transport.
- Smooth ER: Involved in lipid synthesis, and detoxification.
- Golgi Apparatus: Modifies, sorts, and packages proteins for secretion or use within the cell.
- Lysosomes: Contain digestive enzymes to break down waste.
- Chloroplasts (Plant): Perform photosynthesis, converting sunlight to energy (glucose).
- Vacuoles (Plant): Stores water, nutrients, and waste.
- Cell Membrane: Regulates the movement of substances into and out of the cell.
- Cytoplasm: Gel-like substance where organelles are suspended.
- Cell wall (Plant): Provides structural support and protection.
- Large Central Vacuole (Plant): Maintains turgor pressure, stores water, nutrients.
Types of Cell Research
- Microscopy: Allows visualization of cell structures not visible to the naked eye.
- Cell Culture: Growing cells in labs to study behavior, genetics, response to drugs.
- Molecular Biology Techniques: (PCR, Gel Electrophoresis) Enable the study of genetic material and gene expression to understand diseases and develop treatments.
Cells as Open Systems
- Cells exchange materials (nutrients, gases, waste) with environment.
- Cells obtain energy & release energy through metabolism.
- Cells communicate with each other.
- Cells maintain internal stability (homeostasis).
- Cells reproduce (mitosis, meiosis).
- Cells respond to changes in the environment (stimuli).
Calculating Total Magnification
- Total magnification = ocular lens magnification x objective lens magnification.
Estimating Specimen Size
- Specimen size = Field of View Diameter / Number of Specimens Across Diameter
Gene Editing
- Gene editing modifies an organism's DNA.
- CRISPR-Cas9 is a widely used gene-editing technique using guide RNA to target DNA and an enzyme (Cas9) to cut it.
- Applications include treating genetic disorders, studying genes, and creating crops with desirable traits.
HIV (Human Immunodeficiency Virus)
- HIV attacks CD4+ T cells, weakening the immune system, potentially leading to AIDS.
- HIV replicates inside host cells.
- Transmission occurs through blood, semen, vaginal fluids, and breast milk.
- Treatment involves antiretroviral therapy (ART).
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Description
Explore the fundamental concepts of cell theory and the historical debate around spontaneous generation. Understand the experiments conducted by Pasteur and Redi, which challenged the notion of life arising from non-living matter. Test your knowledge on these pivotal scientific discoveries.