Cell Structure and Theory

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Questions and Answers

How does the structure of a cell membrane contribute to its function as a selective barrier?

The phospholipid bilayer with embedded proteins allows selective passage of molecules. Hydrophobic core repels polar molecules, while protein channels facilitate transport.

How do prokaryotic cells ensure resistance to antibiotics in challenging environments?

Plasmid DNA confers unique phenotypic characters, including resistance to antibiotics, enhancing survival in hostile conditions.

Explain why cells are considered the basic units of life and how this relates to the complexity of living organisms.

Cells are the fundamental structural and functional units because they can independently perform essential life functions, highlighting organizational hierarchy.

Describe the roles of rough endoplasmic reticulum and smooth endoplasmic reticulum in protein production and lipid metabolism, respectively?

<p>Rough ER synthesizes and processes proteins assisted by ribosomes, while smooth ER synthesizes lipids and steroids, contributing to cellular functions.</p> Signup and view all the answers

What implications does the presence of a single circular DNA molecule in mitochondria have for mitochondrial inheritance and disease?

<p>The presence of a single circular DNA molecule implies maternal inheritance and increased vulnerability to mutations causing mitochondrial diseases.</p> Signup and view all the answers

Outline the key differences between mitosis and meiosis concerning chromosome number and genetic variation?

<p>Mitosis produces two diploid cells with identical genetic information, while meiosis produces four haploid cells with genetic variation.</p> Signup and view all the answers

Describe the key features and implications of mitochondrial replacement therapy techniques.

<p>Mitochondrial replacement therapy involves replacing defective mitochondria to prevent disease transmission, using pronuclear transfer or reconstructed egg technology.</p> Signup and view all the answers

What is the significance of the fluid mosaic model in describing the structure of the cell membrane?

<p>Illustrates the flexibility and dynamic nature of the cell membrane, allowing for the movement of proteins and lipids which supports various cellular processes.</p> Signup and view all the answers

How does the absence of a nuclear membrane in prokaryotic cells affect genetic material accessibility?

<p>Genetic material is directly exposed to the cytoplasm, facilitating rapid protein synthesis but also heightening its vulnerability to damage. Transcription can happen without needing to exit nucleus.</p> Signup and view all the answers

In what ways do plant cells utilize their cell walls to interact with their environment?

<p>The rigid cell wall provides structural support and protection, facilitates cell-to-cell interaction, and acts as barrier against pathogens.</p> Signup and view all the answers

How does the structural organization of a chloroplast contribute to the process of photosynthesis?

<p>Thylakoid membranes within chloroplasts increase surface area for light absorption, crucial for photosynthesis turning light energy into chemical energy.</p> Signup and view all the answers

What evolutionary advantages do eukaryotes gain through compartmentalization via membrane-bound organelles?

<p>Enhanced efficiency of cellular processes due to spatially separated reactions, allowing specialization and better regulation within a larger, more complex cell.</p> Signup and view all the answers

Examine the role of the Golgi apparatus in modifying and trafficking proteins, considering its significance in cellular functions.

<p>It modifies, sorts, and packages proteins received from the ER, directing them to specific destinations to execute diverse cellular tasks and excrete cellular products.</p> Signup and view all the answers

Assess how mutations in mitochondrial DNA (mtDNA) may lead to various human diseases, considering maternal inheritance patterns.

<p>Mutations lead to energy production deficiency with maternal inheritance as offspring inherit mtDNA from their mother, leading to multisystemic disorders.</p> Signup and view all the answers

Compare and contrast the significance of mitosis versus meiosis in ensuring genetic continuity and variation in organisms.

<p>Mitosis maintains genetic continuity for repair, growth, while meiosis introduces genetic variation for evolution by mixing of genetics between parent.</p> Signup and view all the answers

Describe how the unique structure of sperm cells enhances their function in fertilization.

<p>Sperm cells contains tail for motility. It contains acrosome containing hydrolytic enzymes that helps it to penetrate which aids them in successful fertizilation. Spermatozoa typically contains a single set of chromosome to combine with oocyte chromosome to form zygote.</p> Signup and view all the answers

Analyze the challenges associated with mitochondrial replacement therapy considering ethical concerns and technical complexities.

<p>Concerns include the moral status of modified embryos, potential health risks to the offspring and technical challenges.</p> Signup and view all the answers

Explain the functions of the centrosome and centrioles, and their importance specifically in animal cells.

<p>Animal cells contains centrosome that helps in cell division. Centrioles are composed of one or more chromosome that can only be see under microscope</p> Signup and view all the answers

In the context of DNA barcoding consider whether it is a reliable method of distinguishing species that are closely related, but morphologically similar?

<p>Yes, DNA barcoding is an extremely reliable way to determine even closely related species due to species genetic distinctiveness.</p> Signup and view all the answers

Illustrate how errors during DNA replication can lead to mutations and their subsequent effects on cellular function and organismal health.

<p>Errors during DNA replication can lead to mutations, altering protein structure and cellular function, potentially causing diseases like cancer which can reduce overal organismal health.</p> Signup and view all the answers

Analyze how environmental factors can influence gene expression without altering the DNA sequence of an organism.

<p>Environmental factors influence gene expression via epigenetic modifications, such as DNA methylation. These can then either activate, or inhibite cell function.</p> Signup and view all the answers

How do the unique characteristics of prokaryotic cells enable them to thrive in diverse and extreme environments?

<p>Compact genome with lack of nucleus membrane that facilitates very rapid response with simple regulation and can exist in extreme conditions with ability to acquire resistance.</p> Signup and view all the answers

How does a greater understanding of these biological fundamentals translate to improvement of modern medical technologies and treatments?

<p>Understanding cell biology and genetic can lead to advancements in the medical field that treats illnesses more effectively. It can also help with gene therapy.</p> Signup and view all the answers

Predict how the presence of specific organelles in plant cells uniquely contributes to their survival in an ecosystem.

<p>A presence of chloroplasts that facilitates photosynthesis and cell walls provides structural integrety can greatly contribute to survivial</p> Signup and view all the answers

Formulate a hypothesis on how genetic drift might affect the genetic diversity of a small, isolated population of organisms over time.

<p>Genetic drift can cause the population to have reduced genetic variability so small population over-time have limited genes.</p> Signup and view all the answers

Describe roles of the ribosomes in protein synthesis, comparing its function across both prokaryotic and eukaryotic cells.

<p>Ribosomes in both prokaryotic and eukaryotic cells translate mRNA into proteins, though there are slight structural differeces that helps in the pro</p> Signup and view all the answers

Propose a scenario to explain how the semi-permeable nature of the cell membrane maintains homeostasis in a changing external environment.

<p>The semi-permeable nature allows the cell membrane to only let certain substances pas through to have water balance and nutrient concentration.</p> Signup and view all the answers

Considering cell-to-cell communication, predict how disruptions in cellular signaling pathways can lead to diseases, focusing on an instance where such breakdowns has been investigated.

<p>There are various routes where disruption pathways occur include: neurological disorders that lead to tumor growth.</p> Signup and view all the answers

Explain how viruses utilize host cell machinery for their replication, impacting host cell viability and functions.

<p>Viruses replicate by using host cell replication methods; thus it is replicating at the cost of the host. This causes the cell to not function and the virus kills after production.</p> Signup and view all the answers

Given that mitochondrial diseases often affect multiple organ systems, suggest why these conditions are challenging to diagnose.

<p>Mitochondrial diseases have a lot of different symptoms and because of this complex relationship it can be difficult to diagnose, as these manifest in many organs.</p> Signup and view all the answers

Outline role of the Zono Pellusida's function in egg and what it's structure and composition does for the Oocyte

<p>Zono Pellusida is a protective covering to prevent the oocyte from drying. Also contains glycoprotiens that help with the oocytes processes.</p> Signup and view all the answers

Explain how both sperm cells and oocyte structure impacts polyspermy and how it contributes to healthy process.

<p>Since both must only combine to form a zygote, a successful egg will not combine with anymore. There are various methods, which entail preventing further sperm cells to combine.</p> Signup and view all the answers

Given that Mitochondria DNA (mtDNA) diseases are mostly inherited, why are both Mitochondrial Replacement Therapy and Pronuclear Transfer methods?

<p>The intent of both methods is to eliminate disease inheritances from the oocytes.</p> Signup and view all the answers

Describe in simplistic terms what the "Pronucleus" does (in the scope of oocyte) and why scientists need to manipulate.

<p>The pronucleus houses important genetics and nucleus information. When defective, it must be replaced to continue function or prevent inheritnace.</p> Signup and view all the answers

Describe the ethical and general processes of Pronuclear Transfer and explain if this is generally widespreaded.

<p>It is a complex process that involves medical manipulation of both parents and another donar. As you can imagine, there are long term health and inheritances considerations. Generally not a common treatment.</p> Signup and view all the answers

Why does mitochondrial dysfunction present challenges in medical diagnoses and treatment. Provide specific examples of the affected tissue types to illustrate

<p>Mitochondrial dysfunction is challenging to diagnose and treat. Since it happens in multiple organs that have high energy dependency can be extremely complex.</p> Signup and view all the answers

How can the principles of normal embryological development be utilized to improve the efficacy of in vitro fertilization (IVF) for human infertility. Elaborate on how this ensures future zygote survivability

<p>There would need to be manipulation techniques required for the future zygote. This ensures survivability in the culture, as well as future cells.</p> Signup and view all the answers

Compare the approaches to prevent Mitochondrial related inheritance to 1900 medicine and provide a future insight for how new research may shift techniques.

<p>In the past, many pregnancies would have had negative impacts of still birth or disease-ridden birth. While techniques can vary, as well as new methods will also continue to improve treatments.</p> Signup and view all the answers

Discuss how lifestyle factors and environmental exposures might affect the integrity of a cell's DNA and therefore alter overall human wellbeing and function.

<p>The human genome contains the blueprint for the overall cell system. Lifestyle and exposure such as smoking lead to poor function.</p> Signup and view all the answers

Describe the role of the scientist in current day modern gene editing and how this would benefit scientific progression.

<p>Scientists in modern day have multiple approaches, the main is to prevent, repair and have less down falls of previous generation. Their role is instrumental and the forefront of improving conditions.</p> Signup and view all the answers

How does a cell's structure relate to its function as the fundamental unit of life?

<p>A cell's complete structure is necessary for independent living. It performs essential functions, making it the basic structural and functional unit of all living organisms.</p> Signup and view all the answers

What are the two core tenets of the cell theory as modified by Virchow?

<p>The two core tenets are: (1) all living organisms are composed of cells and their products, and (2) all cells arise from pre-existing cells.</p> Signup and view all the answers

How do eukaryotic and prokaryotic cells differ in terms of their nuclear organization and what terms describe them?

<p>Eukaryotic cells have a membrane-bound nucleus, while prokaryotic cells lack a membrane-bound nucleus. These are described as eukaryotic and prokaryotic, respectively.</p> Signup and view all the answers

What role does the cytoplasm play in both plant and animal cells?

<p>The cytoplasm occupies the volume of the cell and serves as the main arena for cellular activities in both plant and animal cells.</p> Signup and view all the answers

In addition to genomic DNA, what other type of DNA is found in many bacteria, and what unique property does it confer?

<p>Many bacteria have small circular DNA called plasmids, which confer unique phenotypic characters such as antibiotic resistance.</p> Signup and view all the answers

What are the key differences in the DNA replication process between eukaryotic and prokaryotic cells?

<p>Eukaryotic DNA replication is highly regulated with selective origins and sequences, whereas prokaryotic cells replicate the entire genome at once.</p> Signup and view all the answers

How does the structural organization of genetic material differ between eukaryotic and prokaryotic cells?

<p>Eukaryotic cells organize their genetic material into chromosomes, whereas prokaryotic cells have one circular loop of DNA and plasmids.</p> Signup and view all the answers

What is the role of extensive compartmentalization in eukaryotic cells, and how is it achieved?

<p>Extensive compartmentalization organizes the cytoplasm and is achieved through the presence of membrane-bound organelles.</p> Signup and view all the answers

How does the fluid mosaic model describe the structure and function of the cell membrane?

<p>The fluid mosaic model describes the cell membrane as a bilayer of phospholipids. Proteins are embedded within the membrane, allowing for selective permeability and transport.</p> Signup and view all the answers

How are membrane proteins classified based on the ease of their extraction, and where do they typically reside within the membrane?

<p>Membrane proteins are classified as integral and peripheral. Peripheral proteins lie on the surface of the membrane, while integral proteins are partially or totally buried within it.</p> Signup and view all the answers

What advantages does the quasi-fluid nature of the lipid bilayer confer to the cell?

<p>It enables lateral movement of proteins facilitating cell growth, formation of intercellular junctions, secretion, endocytosis, and cell division.</p> Signup and view all the answers

What key roles does the non-living rigid cell wall perform for plant and fungal cells?

<p>The cell wall provides shape to the cell, protects it from mechanical damage and infection, and facilitates cell-to-cell interaction.</p> Signup and view all the answers

What is the primary function of ribosomes, and in which cellular locations are they typically found?

<p>Ribosomes are responsible for protein synthesis, and they are found in all cells.</p> Signup and view all the answers

What is the main purpose of mitochondria, and what unique genetic material do they possess?

<p>Mitochondria are the powerhouse of the cell, producing energy in the form of ATP. They possess a single circular DNA molecule.</p> Signup and view all the answers

How do nuclear pores facilitate communication between the nucleus and cytoplasm?

<p>Nuclear pores serve as passages through which RNA and protein molecules move in both directions between the nucleus and the cytoplasm.</p> Signup and view all the answers

Briefly explain the central dogma of molecular biology, detailing the flow of genetic information.

<p>DNA is transcribed into RNA. Then, RNA is translated into proteins, where proteins (enzymes) perform cellular functions.</p> Signup and view all the answers

What is the significance of having chromosomes that are characteristic for a species?

<p>This is significant as chromosomes ensure genetic consistency and stability, defining the unique traits of each species.</p> Signup and view all the answers

What does it mean for a cell to be diploid or haploid, and how are these states represented?

<p>A diploid cell has chromosomes existing as a pair whereas A haploid cell has only one set. A diploid is represented as 2(n) and haploid is n.</p> Signup and view all the answers

Why is it necessary for gametes to be haploid cells?

<p>In sexual reproduction. The fusion of gametes ensures that the offspring receives the correct number of chromosomes from each parent.</p> Signup and view all the answers

What are the fundamental differences between mitosis and meiosis in terms of their purpose and final products?

<p>Mitosis is for growth producing diploid cells that are required to the cell's life. Meiosis is for reproduction, producing haploid cells required for sexual reproduction.</p> Signup and view all the answers

Describe the process of gametogenesis and the types of cells that are produced as a result.

<p>Gametogenesis is the process of forming gametes (sperm in males and ovum in females) through meiosis, halving the chromosome number.</p> Signup and view all the answers

What is the purpose of the acrosome reaction during fertilization?

<p>The acrosome reaction facilitates sperm penetration through the egg's protective layers.</p> Signup and view all the answers

What are some important features of Zygotes, and how are they related ?

<p>The importance is that both parent chromosomes are passed on to the Zygote. Also, it transfers organelles into the mother.</p> Signup and view all the answers

What is mitochondrial replacement therapy (MRT), and what is its primary purpose?

<p>Mitochondrial replacement therapy is the replacement of mitochondria in one or more cells to prevent or ameliorate disease. It involves replacing defective mitochondria with healthy ones.</p> Signup and view all the answers

Explain how pronuclear transfer works?

<p>The technique will start by removing the pronucleus that contains the mitochondria, which would be unhealthy. After that the other set is put in</p> Signup and view all the answers

What ethical concerns are raised by the creation of 'three-parent babies'?

<p>Creating babies with DNA from three parents involves complex ethical and social considerations, including questions about genetic identity and inheritance.</p> Signup and view all the answers

What key features distinguish plant cells from animal cells?

<p>Plant cells have cell walls and chloroplasts, unlike animal cells.</p> Signup and view all the answers

What cell organelles play play the most significant role protein synthesis?

<p>Endoplasmic reticulum and Ribosome is the correct answer.</p> Signup and view all the answers

What organelles contains DNA other than the nucleus?

<p>Mitochondrion is one of the organelles that contains DNA other than the nucleus.</p> Signup and view all the answers

What statements can be used for DNA Barcoding ?

<p>DNA Barcoding can be used for plants, animals and to identify undesirable materials.</p> Signup and view all the answers

Flashcards

Cell

The fundamental structural and functional unit of all living organisms, capable of independent existence and performing essential functions.

Cell Theory

States that all living organisms are composed of cells and their products, and that all cells arise from pre-existing cells.

Nucleus

A dense, membrane-bound structure within a cell containing chromosomes with DNA.

Eukaryotic Cells

Cells with membrane-bound nuclei.

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Prokaryotic Cells

Cells lacking a membrane-bound nucleus.

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Cytoplasm

A semi-fluid matrix within cells occupying the volume, the main cellular activity site.

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Ribosomes

Non-membrane bound organelles found in all cells, responsible for protein synthesis.

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Prokaryotic examples

Bacteria, blue-green algae, mycoplasma and PPLO.

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Eukaryotic feature

Extensive compartmentalisation of cytoplasm through the presence of membrane bound organelles.

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Cell Wall

A non-living rigid structure forming an outer covering for the plasma membrane in fungi and plants.

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Plant cell wall

Has Cellulose, hemicellulose, pectins and proteins

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Cell walls functions

Outer porous covering that has shape, Protects it from Mechanical Damage and Infection

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Cell Organelles

Structures within a cell with specific functions like digestion, energy production, and packaging

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Rough Endoplasmic Reticulum

An organelle that transports or synthesize transports protein.

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Lysosomes

An organelle that responsible for digestion.

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Smooth Endoplasmic Reticulum

An organelle that forms proteins and does transport in cell.

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Golgi Apparatus

Responsible for packaging within a cell.

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Storage

Serves for packaging Storage in cell.

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Mitochondria

An organelle that produces ATP that produces energy in form of ATP.

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Chloroplast

Photosynthesis of cell function.

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Centrosome

Cell division in animals.

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Nuclear Pores

The passages movement of RNA and protein molecules takes place in both directions between the nucleus and the cytoplasm.

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Nuclear matrix

Nucleoplasm and Chromatin

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Nucleolus

A continuous structure with the rest of the nucleoplasm as it is not a membrane bound structure that active ribosomal RNA synthesis.

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Chromosomes

Humans => cells => DNA => chromatin, that has Chromosomes.

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Humans and cells

Humans => Made up of cells.

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Haploid

A single copy of chromosomes

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Diploid

Double copy of chromosomes.

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Cell division

cells must divide and grow so that daughter cells have equal number of Chromosomes after division/ Mitosis.

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Sperm and Aum

Sperm fertilize Aum make offspring.

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Mitosis vs Meiosis

For growth = Mitosis For reproduction= Meiosis.

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Mitosis vs Meiosis pt2

Mitosis creates the diploid where Meiosis creates the haploid/

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Diploid produces the cells while haploid makes 1/2 DNA

Diploid produces cells while haploid makes 1/2 DNA

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Gametes Functions

Cells that contain haploid cells that form together during intercourse to make offspring.

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Zygote Features

Contain organelles, and nuclei containing chromosomes from both parents.

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Replace mother defective mitochondria

Replace a mother's defective mitochondria with either Pronuclear Transfer or Reconstructed Egg Technology.

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Reconstructed Egg Technology

Pronuclear transfer uses After Fertilisation. Where Reconstructed Egg Technology does Before Fertilisation.

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3 parents offspring

Pronuclear transfer and reconstructed egg technology leads to 3 parents offspring.

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Pronuclear transfer

Involves transfer of pronuclei from one zygote to another to treat mtDNA disease

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Plant and animal differences.

In plant cells, cellulose cell walls whilst animal cells do not exist, and both animal or Plant cells have plasma membrane.

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Plant and animal vacuoles.

Mature plant cell has one large vacuole whilst animal cell has many small vacuoles.

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Other Parts vs, Protien

Lysosome and Centrosome

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Organelle DNA

Mitochondrion

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DNA barcoding

Genetic identification that is based on distinguishing among species that look alike 2. Identify undesirable animal or plant materials in processed foods.

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Study Notes

Cell: The Fundamental Unit of Life

  • Unicellular organisms exhibit independent existence.
  • They also perform life's essential functions.
  • A complete cell structure is essential for independent living.
  • Cells are the fundamental structural and functional units of all living organisms.
  • Anton Von Leeuwenhoek was the first to observe and describe a live cell.
  • Robert Brown discovered the nucleus.

Cell Theory

  • The cell theory was formulated by Schleiden and Schwann and later modified by Virchow.
  • All living organisms consist of cells and their products.
  • All cells arise from pre-existing cells.

Basic Structure of a Cell

  • Each cell has a nucleus, a dense, membrane-bound structure.
  • The nucleus contains chromosomes, the structures that contain genetic material, like DNA.
  • Cells with membrane-bound nuclei are called eukaryotic cells.
  • Cells lacking membrane-bound nuclei are called prokaryotic cells.
  • Cytoplasm, a semi-fluid matrix, occupies the volume both prokaryotic and eukaryotic cells.
  • The cytoplasm is the main site of cellular activities in both plant and animal cells.
  • Prokaryotic cells are in the kingdom Monera.
  • Ribosomes are non-membrane-bound organelles found in all cells.
  • The term prokaryotic can be broken down to understand it: Pro + Eu + karyon

Prokaryotic Cells

  • Examples of prokaryotic cells include bacteria, blue-green algae, mycoplasma, and PPLO.
  • Prokaryotes have a cell wall around the cell membrane, except for mycoplasma.
  • Cytoplasm fills the fluid matrix of the cell.
  • Prokaryotes lack a well-defined nucleus.
  • The genetic material is naked and not enveloped by a nuclear membrane.
  • Genomic DNA in bacteria is typically one circular chromosome.
  • Many bacteria have smaller circular DNA molecules called plasmids outside the genomic DNA.
  • Plasmid DNA gives unique phenotypic characteristics like antibiotic resistance.
  • Nuclear membranes are only present in eukaryotic cells.

Eukaryotic Cells

  • Eukaryotic cells have extensive compartmentalization of cytoplasm.
  • This compartmentalization occurs through the presence of membrane-bound organelles.
  • Eukaryotic cells possess an organized nucleus with a nuclear envelope.
  • The genetic material in eukaryotic cells is organized into chromosomes.
  • There are 5 kingdoms based on their features: Monera, Protista, Fungi, Plantae, and Animalia.

Cell Membrane

  • Mainly composed of lipids and proteins.
  • The major lipids are phospholipids, arranged in a bilayer.
  • Membrane proteins are classified as integral or peripheral depending on the ease of extraction.
  • Peripheral proteins lie on the surface of the membrane.
  • Integral proteins are partially or totally buried in the membrane.
  • The quasi-fluid nature of the lipid enables lateral movement of proteins.
  • It is also enabling cell growth, formation of intercellular junctions, secretion, endocytosis, cell division, etc.
  • The membrane is selectively permeable to some molecules.
  • Molecules on either side of it are subject to passive and active transport.

Cell Wall

  • The cell wall is a non-living rigid structure around the plasma membrane
  • It is found in fungi and plants.
  • The cell wall provides shape to the cell.
  • It also protects the cell from mechanical damage and infection.
  • The cell wall facilitates cell-to-cell interaction.
  • It acts as a barrier to undesirable macromolecules.
  • Algae cell walls are made of cellulose, galactans, and mannans.
  • Plant cell walls consist of cellulose, hemicellulose, pectins, and proteins.

Nucleus

  • Nuclear pores are passages for RNA and protein molecule movement between the nucleus and cytoplasm.
  • The nuclear matrix, or nucleoplasm, contains the nucleolus and chromatin.
  • The nucleolus is continuous with the rest of the nucleoplasm and is not membrane-bound.
  • One of the functions of Nucleolus is the site for active ribosomal RNA synthesis.
  • During cell division, cells show structured chromosomes in place of the nucleus.

Mitochondrial Replacement Therapy (MRT)

  • Replaces defective mitochondria with healthy mitochondria.
  • Also known as mitochondrial donation: replaces mitochondria in one or more cells to prevent or ameliorate diseases.
  • MRT originated as a special form of in vitro fertilization.
  • All or some of the future baby's mitochondrial DNA (mtDNA) comes from a third party,
  • This technique is used when mothers carry genes for mitochondrial diseases.

Pronuclear Transfer

  • This involves the transfer of pronuclei from one zygote to another AFTER fertilization.

Reconstructed Egg Technology

  • This is done BEFORE fertilization

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